Construct: ORF TRCN0000476345
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005832.1_s317c1
- Derived from:
- ccsbBroadEn_06971
- DNA Barcode:
- AACTTACATGAACCCCTCCTAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC20A2 (6575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476345
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6575 | SLC20A2 | solute carrier family 20 me... | NM_001257180.2 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 2 | human | 6575 | SLC20A2 | solute carrier family 20 me... | NM_001257181.1 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 3 | human | 6575 | SLC20A2 | solute carrier family 20 me... | NM_006749.5 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 4 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_005273613.4 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 5 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_017013748.1 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 6 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_024447235.1 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 7 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_024447236.1 | 99.8% | 99.6% | 1940G>T;1943T>C |
| 8 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_005273615.4 | 92.7% | 92.4% | (many diffs) |
| 9 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_006716390.4 | 92.6% | 92.4% | 286_287ins141;1799G>T;1802T>C |
| 10 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_017013749.2 | 92.6% | 92.4% | 286_287ins141;1799G>T;1802T>C |
| 11 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_024447237.1 | 92.6% | 92.4% | 286_287ins141;1799G>T;1802T>C |
| 12 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_006716391.4 | 78.8% | 78.6% | 0_1ins411;1529G>T;1532T>C |
| 13 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_017013750.2 | 78.8% | 78.6% | 0_1ins411;1529G>T;1532T>C |
| 14 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_017013751.2 | 78.8% | 78.6% | 0_1ins411;1529G>T;1532T>C |
| 15 | human | 6575 | SLC20A2 | solute carrier family 20 me... | XM_017013752.2 | 78.8% | 78.6% | 0_1ins411;1529G>T;1532T>C |
| 16 | mouse | 20516 | Slc20a2 | solute carrier family 20, m... | NM_011394.3 | 82.1% | 91% | (many diffs) |
| 17 | mouse | 20516 | Slc20a2 | solute carrier family 20, m... | XM_006509043.2 | 82.1% | 91% | (many diffs) |
| 18 | mouse | 20516 | Slc20a2 | solute carrier family 20, m... | XM_006509044.1 | 82.1% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2022
- ORF length:
- 1956
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catggatgag tatttgtgga tggtcatttt gggtttcatc atagctttca 121 tcttggcctt ttctgttggt gcaaacgatg ttgccaactc ctttggtaca gccgtgggct 181 ctggtgtggt gaccttgagg caggcatgca ttttagcttc aatatttgaa accaccggct 241 ccgtgttact aggcgccaaa gtaggagaaa ccattcgcaa aggtatcatt gacgtgaacc 301 tgtacaacga gacggtggag actctcatgg ctggggaagt tagtgccatg gttggttccg 361 ctgtgtggca gctgattgct tccttcctga ggcttccaat ctcaggaacg cactgcattg 421 tgggttctac tataggattc tcactggtcg caatcggtac caaaggtgtg cagtggatgg 481 agcttgtcaa gattgttgct tcttggttta tatctccact gttgtctggt ttcatgtctg 541 gcctgctgtt tgtactcatc agaattttca tcttaaaaaa ggaagaccct gttcccaatg 601 gcctccgggc actcccagta ttctatgctg ctaccatagc aatcaatgtc ttttccatca 661 tgtacacagg agcaccagtg ctcggccttg ttctccccat gtgggccata gccctcattt 721 cctttggtgt cgccctcctg ttcgcttttt ttgtgtggct cttcgtgtgt ccgtggatgc 781 ggaggaaaat aacaggcaaa ttacaaaaag aaggtgcttt atcacgagta tctgacgaaa 841 gcctcagtaa ggttcaggaa gcagagtccc cagtatttaa agagctacca ggtgccaagg 901 ctaatgatga cagcaccatc ccgctcacgg gagcagcagg ggagacactg gggacctcgg 961 aaggcacttc tgcgggcagc caccctcggg ctgcatacgg aagagcactg tccatgaccc 1021 atggctctgt gaaatcgccc atctccaacg gcaccttcgg cttcgacggc cacaccagga 1081 gcgacggtca tgtgtaccac accgtgcaca aagactcggg gctctacaaa gatctgctgc 1141 acaaaatcca catcgacagg ggccccgagg agaagccagc ccaggaaagc aactaccggc 1201 tgctgcgccg aaacaacagt tacacctgct acaccgcagc catttgtggg ctgccagtgc 1261 acgccacctt tcgagctgcg gactcatcgg ccccagagga cagtgagaag ctggtgggcg 1321 acaccgtgtc ctactccaag aagaggctgc gctacgacag ctactcgagc tactgtaacg 1381 cggtggcaga ggcggagatc gaggcggagg agggcggcgt ggagatgaag ctggcgtcgg 1441 agctggccga ccctgaccag ccgcgagagg accctgcaga ggaggagaag gaggagaagg 1501 acgcacccga ggttcacctc ctgttccatt tcctgcaggt cctcaccgcc tgtttcgggt 1561 cctttgctca cggcggcaat gacgtgagta atgccatcgg tcccctggta gccttgtggc 1621 tgatttacaa acaaggcggg gtaacgcaag aagcagctac acccgtctgg ctgctgtttt 1681 atggaggagt tggaatctgc acaggcctct gggtctgggg GAGAAGAGTG ATCCAGACCA 1741 TGGGGAAGGA CCTCACTCCC ATCACGCCGT CCAGCGGCTT CACGATCGAG CTGGCCTCAG 1801 CCTTCACAGT GGTGATCGCC TCCAACATCG GGCTTCCAGT CAGCACCACG CACTGTAAGG 1861 TGGGCTCGGT GGTGGCCGTG GGCTGGATCC GCTCCCGCAA GGCTGTGGAC TGGCGCCTCT 1921 TTCGGAACAT CTTCGTGGCC TGGTTCGTGA CCGTCCCTGT GGCTGGGCTG TTCAGCGCTG 1981 CTGTCATGGC TCTTCTCATG TATGTGACCC TTCCATATGT GTGCCCAACT TTCTTGTACA 2041 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2101 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2161 AGGACGAAAC TTACATGAAC CCCTCCTAAC TACGCGTTAA GTCgacaatc aacctctgga 2221 ttacaaaatt tgtgaaagat t