Construct: ORF TRCN0000476350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005644.1_s317c1
- Derived from:
- ccsbBroadEn_05848
- DNA Barcode:
- CACCAACCGCACCCAGCTGTATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP6 (395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476350
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 395 | ARHGAP6 | Rho GTPase activating prote... | NM_013427.3 | 99.9% | 99.7% | 814G>T;2373C>G |
| 2 | human | 395 | ARHGAP6 | Rho GTPase activating prote... | NM_001287242.2 | 81% | 79.6% | (many diffs) |
| 3 | human | 395 | ARHGAP6 | Rho GTPase activating prote... | NM_013423.3 | 79% | 78.9% | 0_1ins609;205G>T;1764C>G |
| 4 | human | 395 | ARHGAP6 | Rho GTPase activating prote... | NM_006125.3 | 76.8% | 75.8% | (many diffs) |
| 5 | human | 395 | ARHGAP6 | Rho GTPase activating prote... | NR_109776.2 | 52.8% | (many diffs) | |
| 6 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | NM_009707.4 | 83.9% | 84.7% | (many diffs) |
| 7 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | XM_006528694.1 | 83.9% | 84.7% | (many diffs) |
| 8 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | NM_178754.3 | 69.2% | 68.6% | (many diffs) |
| 9 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | NM_001287530.1 | 68% | 68.6% | (many diffs) |
| 10 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | XM_011247783.1 | 59% | 61.1% | (many diffs) |
| 11 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | NR_109847.1 | 47.8% | (many diffs) | |
| 12 | mouse | 11856 | Arhgap6 | Rho GTPase activating prote... | XR_878255.2 | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2991
- ORF length:
- 2922
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccgcgcag agcctgctcc acagcgtctt ctcctgttcc tcgcccgctt 121 caagtagcgc ggcctcggcc aagggcttct ccaagaggaa gctgcgccag acccgcagcc 181 tggacccggc cctgatcggc ggctgcggga gcgacgaggc gggcgcggag ggcagtgcgc 241 ggggagccac ggcgggccgc ctctactccc catcactccc agccgagagt ctcggccctc 301 gcttggcgtc ctcttcccgg ggtccgcccc ccagggccac caggctaccg cctcctggac 361 ctctttgctc gtccttctcc acacccagca ccccgcagga gaagtcacca tccggcagct 421 ttcactttga ctatgaggtt cccctgggtc gcggcggcct caagaagagc atggcctggg 481 acctgccttc tgtcctggcc gggccagcca gtagccgaag cgcttccagc atcctctgtt 541 catccggggg aggccccaat ggcatcttcg cttctcctag gaggtggctc cagcagagga 601 agttccagtc cccacccgac agtcgcgggc acccctacgt cgtgtggaaa tccgagggtg 661 atttcacctg gaacagcatg tcaggccgca gtgtacggct gaggtcagtc cccatccaga 721 gtctctcaga gctggagagg gcccggctgc aggaagtggc tttttatcag ttgcaacagg 781 actgtgacct gagctgtcag atcaccattc ccaaagatgg acaaaagaga aagaaatctt 841 taagaaagaa actggattca ctaggaaagg agaaaaacaa atacaaagaa ttcatcccac 901 aggcatttgg aatgccctta tcccaagtca ttgcgaatga cagggcctat aaactcaagc 961 aggacttgca gagggacgag cagaaagatg catctgactt tgtggcttcc ctcctcccat 1021 ttggaaataa aagacaaaac aaagaactct caagcagtaa ctcatctctc agctcaacct 1081 cagaaacacc gaatgagtca acgtccccaa acaccccgga accggctcct cgggctagga 1141 ggaggggtgc catgtcagtg gattctatca ccgatcttga tgacaatcag tctcgactac 1201 tagaagcttt acaactttcc ttgcctgctg aggctcaaag taaaaaggaa aaagccagag 1261 ataagaaact cagtctgaat cctatttaca gacaggtccc taggctggtg gacagctgct 1321 gtcagcacct agaaaaacat ggcctccaga cagtggggat attccgagtt ggaagctcaa 1381 aaaagagagt gagacaatta cgtgaggaat ttgaccgtgg gattgatgtc tctctggagg 1441 aggagcacag tgttcatgat gtggcagcct tgctgaaaga gttcctgagg gacatgccag 1501 acccccttct caccagggag ctgtacacag ctttcatcaa cactctcttg ttggagccgg 1561 aggaacagct gggcaccttg cagctcctca tataccttct acctccctgc aactgcgaca 1621 ccctccaccg cctgctacag ttcctctcca tcgtggccag gcatgccgat gacaacatca 1681 gcaaagatgg gcaagaggtc actgggaata aaatgacatc tctaaactta gccaccatat 1741 ttggacccaa cctgctgcac aagcagaagt catcagacaa agaattctca gttcagagtt 1801 cagcccgggc tgaggagagc acggccatca tcgctgttgt gcaaaagatg attgaaaatt 1861 atgaagccct gttcatggtt cccccagatc tccagaacga agtgctgatc agcctgttag 1921 agaccgatcc tgatgtcgtg gactatttac tcagaagaaa ggcttcccaa tcatcaagcc 1981 ctgacatgct gcagtcggaa gtttcctttt ccgtgggagg gaggcattca tctacagact 2041 ccaacaaggc ctccagcgga gacatctccc cttatgacaa caactcccca gtgctgtctg 2101 agcgctccct gctggctatg caagaggacg cggccccggg gggctcggag aagctttaca 2161 gagtgccagg gcagtttatg ctggtgggcc acttgtcgtc gtcaaagtca agggaaagtt 2221 ctcctggacc aaggcttggg aaagatctgt cagaggagcc tttcgatatc tggggaactt 2281 ggcattcaac attaaaaagc ggatccaaag acccaggaat gacaggttcc tctggagaca 2341 tttttgaaag cagctcccta agagcggggc cctgctccct ttctcaaggg aacctgtccc 2401 caaattggcc tcggtggcag gggagccccg cagagctgga gagcgacacg cagggggctc 2461 ggaggactca ggccgcagcc cccgcgacgg agggcagggc ccaccctgcg gtgtcgcgcg 2521 cctgcagcac gccccacgtc caggtggcag ggaaagccga gcggcccacg gccaggtcgg 2581 agcagtactt gaccctgagc ggcgcccacg acctcagcga gagtgagctg gatgtggccg 2641 ggctgcagag ccgggccaca cctCAGTGCC AAAGACCCCA TGGGAGTGGG AGGGATGACA 2701 AGCGGCCCCC GCCTCCATAC CCGGGCCCAG GGAAGCCCGC GGCAGCGGCA GCCTGGATCC 2761 AGGGGCCCCC GGAAGGCGTG GAGACACCCA CGGACCAGGG AGGCCAAGCA GCCGAGCGAG 2821 AGCAGCAGGT CACGCAGAAA AAACTGAGCA GCGCCAACTC CCTGCCAGCG GGCGAGCAGG 2881 ACAGTCCGCG CCTGGGGGAC GCTGGCTGGC TCGACTGGCA GAGAGAGCGC TGGCAGATCT 2941 GGGAGCTCCT GTCGACCGAC AACCCCGATG CCCTGCCCGA GACGCTGGTC TTGCCAACTT 3001 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3061 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3121 CTTGTGGAAA GGACGACACC AACCGCACCC AGCTGTATTC ACGCGTTAAG TCgacaatca 3181 acctctggat tacaaaattt gtgaaagatt