Construct: ORF TRCN0000476350

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005644.1_s317c1
Derived from:
ccsbBroadEn_05848
DNA Barcode:
CACCAACCGCACCCAGCTGTATTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGAP6 (395)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476350
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 395 ARHGAP6 Rho GTPase activating prote... NM_013427.3 99.9% 99.7% 814G>T;2373C>G
2 human 395 ARHGAP6 Rho GTPase activating prote... NM_001287242.2 81% 79.6% (many diffs)
3 human 395 ARHGAP6 Rho GTPase activating prote... NM_013423.3 79% 78.9% 0_1ins609;205G>T;1764C>G
4 human 395 ARHGAP6 Rho GTPase activating prote... NM_006125.3 76.8% 75.8% (many diffs)
5 human 395 ARHGAP6 Rho GTPase activating prote... NR_109776.2 52.8% (many diffs)
6 mouse 11856 Arhgap6 Rho GTPase activating prote... NM_009707.4 83.9% 84.7% (many diffs)
7 mouse 11856 Arhgap6 Rho GTPase activating prote... XM_006528694.1 83.9% 84.7% (many diffs)
8 mouse 11856 Arhgap6 Rho GTPase activating prote... NM_178754.3 69.2% 68.6% (many diffs)
9 mouse 11856 Arhgap6 Rho GTPase activating prote... NM_001287530.1 68% 68.6% (many diffs)
10 mouse 11856 Arhgap6 Rho GTPase activating prote... XM_011247783.1 59% 61.1% (many diffs)
11 mouse 11856 Arhgap6 Rho GTPase activating prote... NR_109847.1 47.8% (many diffs)
12 mouse 11856 Arhgap6 Rho GTPase activating prote... XR_878255.2 44.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2991
ORF length:
2922
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtccgcgcag agcctgctcc acagcgtctt ctcctgttcc tcgcccgctt
121caagtagcgc ggcctcggcc aagggcttct ccaagaggaa gctgcgccag acccgcagcc
181tggacccggc cctgatcggc ggctgcggga gcgacgaggc gggcgcggag ggcagtgcgc
241ggggagccac ggcgggccgc ctctactccc catcactccc agccgagagt ctcggccctc
301gcttggcgtc ctcttcccgg ggtccgcccc ccagggccac caggctaccg cctcctggac
361ctctttgctc gtccttctcc acacccagca ccccgcagga gaagtcacca tccggcagct
421ttcactttga ctatgaggtt cccctgggtc gcggcggcct caagaagagc atggcctggg
481acctgccttc tgtcctggcc gggccagcca gtagccgaag cgcttccagc atcctctgtt
541catccggggg aggccccaat ggcatcttcg cttctcctag gaggtggctc cagcagagga
601agttccagtc cccacccgac agtcgcgggc acccctacgt cgtgtggaaa tccgagggtg
661atttcacctg gaacagcatg tcaggccgca gtgtacggct gaggtcagtc cccatccaga
721gtctctcaga gctggagagg gcccggctgc aggaagtggc tttttatcag ttgcaacagg
781actgtgacct gagctgtcag atcaccattc ccaaagatgg acaaaagaga aagaaatctt
841taagaaagaa actggattca ctaggaaagg agaaaaacaa atacaaagaa ttcatcccac
901aggcatttgg aatgccctta tcccaagtca ttgcgaatga cagggcctat aaactcaagc
961aggacttgca gagggacgag cagaaagatg catctgactt tgtggcttcc ctcctcccat
1021ttggaaataa aagacaaaac aaagaactct caagcagtaa ctcatctctc agctcaacct
1081cagaaacacc gaatgagtca acgtccccaa acaccccgga accggctcct cgggctagga
1141ggaggggtgc catgtcagtg gattctatca ccgatcttga tgacaatcag tctcgactac
1201tagaagcttt acaactttcc ttgcctgctg aggctcaaag taaaaaggaa aaagccagag
1261ataagaaact cagtctgaat cctatttaca gacaggtccc taggctggtg gacagctgct
1321gtcagcacct agaaaaacat ggcctccaga cagtggggat attccgagtt ggaagctcaa
1381aaaagagagt gagacaatta cgtgaggaat ttgaccgtgg gattgatgtc tctctggagg
1441aggagcacag tgttcatgat gtggcagcct tgctgaaaga gttcctgagg gacatgccag
1501acccccttct caccagggag ctgtacacag ctttcatcaa cactctcttg ttggagccgg
1561aggaacagct gggcaccttg cagctcctca tataccttct acctccctgc aactgcgaca
1621ccctccaccg cctgctacag ttcctctcca tcgtggccag gcatgccgat gacaacatca
1681gcaaagatgg gcaagaggtc actgggaata aaatgacatc tctaaactta gccaccatat
1741ttggacccaa cctgctgcac aagcagaagt catcagacaa agaattctca gttcagagtt
1801cagcccgggc tgaggagagc acggccatca tcgctgttgt gcaaaagatg attgaaaatt
1861atgaagccct gttcatggtt cccccagatc tccagaacga agtgctgatc agcctgttag
1921agaccgatcc tgatgtcgtg gactatttac tcagaagaaa ggcttcccaa tcatcaagcc
1981ctgacatgct gcagtcggaa gtttcctttt ccgtgggagg gaggcattca tctacagact
2041ccaacaaggc ctccagcgga gacatctccc cttatgacaa caactcccca gtgctgtctg
2101agcgctccct gctggctatg caagaggacg cggccccggg gggctcggag aagctttaca
2161gagtgccagg gcagtttatg ctggtgggcc acttgtcgtc gtcaaagtca agggaaagtt
2221ctcctggacc aaggcttggg aaagatctgt cagaggagcc tttcgatatc tggggaactt
2281ggcattcaac attaaaaagc ggatccaaag acccaggaat gacaggttcc tctggagaca
2341tttttgaaag cagctcccta agagcggggc cctgctccct ttctcaaggg aacctgtccc
2401caaattggcc tcggtggcag gggagccccg cagagctgga gagcgacacg cagggggctc
2461ggaggactca ggccgcagcc cccgcgacgg agggcagggc ccaccctgcg gtgtcgcgcg
2521cctgcagcac gccccacgtc caggtggcag ggaaagccga gcggcccacg gccaggtcgg
2581agcagtactt gaccctgagc ggcgcccacg acctcagcga gagtgagctg gatgtggccg
2641ggctgcagag ccgggccaca cctCAGTGCC AAAGACCCCA TGGGAGTGGG AGGGATGACA
2701AGCGGCCCCC GCCTCCATAC CCGGGCCCAG GGAAGCCCGC GGCAGCGGCA GCCTGGATCC
2761AGGGGCCCCC GGAAGGCGTG GAGACACCCA CGGACCAGGG AGGCCAAGCA GCCGAGCGAG
2821AGCAGCAGGT CACGCAGAAA AAACTGAGCA GCGCCAACTC CCTGCCAGCG GGCGAGCAGG
2881ACAGTCCGCG CCTGGGGGAC GCTGGCTGGC TCGACTGGCA GAGAGAGCGC TGGCAGATCT
2941GGGAGCTCCT GTCGACCGAC AACCCCGATG CCCTGCCCGA GACGCTGGTC TTGCCAACTT
3001TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
3061CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
3121CTTGTGGAAA GGACGACACC AACCGCACCC AGCTGTATTC ACGCGTTAAG TCgacaatca
3181acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)