Construct: ORF TRCN0000476382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006048.1_s317c1
- Derived from:
- ccsbBroadEn_09384
- DNA Barcode:
- GCCGCCGGCAGGTACTAGGAGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCD3 (113026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113026 | PLCD3 | phospholipase C delta 3 | NM_133373.5 | 99.9% | 100% | 1450T>C |
| 2 | human | 113026 | PLCD3 | phospholipase C delta 3 | XM_024450554.1 | 94.5% | 94.6% | 1450T>C;1709_1843del |
| 3 | human | 113026 | PLCD3 | phospholipase C delta 3 | XM_011524253.3 | 60.2% | 59.8% | (many diffs) |
| 4 | mouse | 72469 | Plcd3 | phospholipase C, delta 3 | NM_152813.3 | 85.4% | 88% | (many diffs) |
| 5 | mouse | 72469 | Plcd3 | phospholipase C, delta 3 | XM_006534308.2 | 85.3% | 87.9% | (many diffs) |
| 6 | mouse | 72469 | Plcd3 | phospholipase C, delta 3 | XM_006534309.3 | 81.3% | 84% | (many diffs) |
| 7 | mouse | 72469 | Plcd3 | phospholipase C, delta 3 | XM_006534310.3 | 79.4% | 82.5% | (many diffs) |
| 8 | mouse | 72469 | Plcd3 | phospholipase C, delta 3 | XM_017314789.1 | 48.1% | 49.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2436
- ORF length:
- 2367
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgtgcggc cgctggaggc gttgccgccg cccgcccgag gagcccccgg 121 tggccgccca ggtcgcagcc caagtcgcgg cgccggtcgc tctcccgtcc ccgccgactc 181 cctccgatgg cggcaccaag aggcccgggc tgcgggcgct gaagaagatg ggcctgacgg 241 aggacgagga cgtgcgcgcc atgctgcggg gctcccggct ccgcaagatc cgctcgcgca 301 cgtggcacaa ggagcggctg taccggctgc aggaggacgg cctgagcgtg tggttccagc 361 ggcgcatccc gcgtgcgcca tcgcagcaca tcttcttcgt gcagcacatc gaggcggtcc 421 gcgagggcca ccagtccgag ggcctgcggc gcttcggggg tgccttcgcg ccagcgcgct 481 gcctcaccat cgccttcaag ggccgccgca agaacctgga cctggcggcg cccacggctg 541 aggaagcgca gcgctgggtg cgcggtctga ccaagctccg cgcgcgcctg gacgccatga 601 gccagcgcga gcggctagac cactggatcc actcctatct gcaccgggct gactccaacc 661 aggacagcaa gatgagcttc aaggagatca agagcctgct gagaatggtc aacgtggaca 721 tgaacgacat gtacgcctac ctcctcttca aggagtgtga ccactccaac aacgaccgtc 781 tagagggggc tgagatcgag gagttcctgc ggcggctgct gaagcggccg gagctggagg 841 agatcttcca tcagtactcg ggcgaggacc gcgtgctgag tgcccctgag ctgctggagt 901 tcctggagga ccagggcgag gagggcgcca cactggcccg cgcccagcag ctcattcaga 961 cctatgagct caacgagaca gccaagcagc atgagctgat gacactggat ggcttcatga 1021 tgtacctgtt gtcgccggag ggggctgcct tggacaacac ccacacgtgt gtgttccagg 1081 acatgaacca gccccttgcc cactacttca tctcttcctc ccacaacacc tatctgactg 1141 actcccagat cggggggccc agcagcaccg aggcctatgt tagggccttt gcccagggat 1201 gccgctgcgt ggagctggac tgctgggagg ggccaggagg ggagcccgtc atctatcatg 1261 gccataccct cacctccaag attctcttcc gggacgtggt ccaagccgtg cgcgaccatg 1321 ccttcacgct gtccccttac cctgtcatcc tatccctgga gaaccactgc gggctggagc 1381 agcaggctgc catggcccgc cacctctgca ccatcctggg ggacatgctg gtgacacagg 1441 cgctggactc cccaaatccc gaggagctgc catccccaga gcagctgaag ggccgggtcc 1501 tggtgaaggg aaagaagctg cccgctgctc ggagcgagga tggccgggct ctgtcggatc 1561 gggaggagga ggaggaggat gacgaggagg aagaagagga ggtggaggct gcagcgcaga 1621 ggcggctggc caagcagatc tccccggagc tgtcggccct ggctgtgtac tgccacgcca 1681 cccgcctgcg gaccctgcac cctgccccca acgccccaca accctgccag gtcagctccc 1741 tcagcgagcg caaagccaag aaactcattc gggaggcagg gaacagcttt gtcaggcaca 1801 atgcccgcca gctgacccgc gtgtacccgc tggggctgcg gatgaactca gccaactaca 1861 gtccccagga gatgtggaac tcgggctgtc agctggtggc cttgaacttc cagacgccag 1921 gctacgagat ggacctcaat gccgggcgct tcctagtcaa tgggcagtgt ggctacgtcc 1981 taaaacctgc ctgcctgcgg caacctgact cgacctttga ccccgagtac ccaggacctc 2041 ccagaaccac tctcagcatc caggtgctga ctgcacagca gctgcccaag ctgaatgccg 2101 agaagccaca ctccattgtg gacccccTGG TGCGCATTGA GATCCATGGG GTGCCCGCAG 2161 ACTGTGCCCG GCAGGAGACT GACTACGTGC TCAACAATGG CTTCAACCCC CGCTGGGGGC 2221 AGACCCTGCA GTTCCAGCTG CGGGCTCCGG AGCTGGCACT GGTCCGGTTT GTGGTGGAAG 2281 ATTATGACGC CACCTCCCCC AATGACTTTG TGGGCCAGTT TACACTGCCT CTTAGCAGCC 2341 TAAAGCAAGG GTACCGCCAC ATACACCTGC TTTCCAAGGA CGGGGCCTCA CTGTCACCAG 2401 CCACGCTCTT CATCCAAATC CGCATCCAGC GCTCCTTGCC AACTTTCTTG TACAAAGTGG 2461 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2521 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2581 AGCCGCCGGC AGGTACTAGG AGAGCACGCG TTAAGTCgac aatcaacctc tggattacaa 2641 aatttgtgaa agatt