Construct: ORF TRCN0000476411
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005357.1_s317c1
- Derived from:
- ccsbBroadEn_13990
- DNA Barcode:
- GTACTTGAACTTTGCCACTTTCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AP3B2 (8120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476411
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8120 | AP3B2 | adaptor related protein com... | NM_004644.5 | 99.9% | 80.1% | 2575_2576insT |
| 2 | human | 8120 | AP3B2 | adaptor related protein com... | NM_001278512.2 | 98.2% | 78.7% | 1969_2025del;2632_2633insT |
| 3 | human | 8120 | AP3B2 | adaptor related protein com... | NM_001278511.1 | 97% | 77.1% | 262_263ins96;2479_2480insT |
| 4 | human | 8120 | AP3B2 | adaptor related protein com... | NM_001348441.1 | 13.3% | 3.2% | 0_1ins2812 |
| 5 | human | 8120 | AP3B2 | adaptor related protein com... | NM_001348440.1 | 12.2% | 14.1% | (many diffs) |
| 6 | mouse | 11775 | Ap3b2 | adaptor-related protein com... | NM_021492.3 | 88.9% | 76.1% | (many diffs) |
| 7 | mouse | 11775 | Ap3b2 | adaptor-related protein com... | XM_006540571.3 | 87.4% | 74.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2766
- ORF length:
- 2697
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggccgcc cccgcctaca gcgaagacaa gggcggctcc gctggccccg 121 gggagcccga gtacggccac gaccccgcga gcggcggcat cttctcctcc gactacaagc 181 ggcatgatga cctgaaggag atgctggaca ccaacaagga ttctctcaag ctggaggcca 241 tgaagaggat tgtggcgatg attgcccgag gaaagaatgc ttcagacctg tttcccgcgg 301 tggtgaagaa cgtggcctgt aagaacatag aggtgaagaa gcttgtctat gtgtacctgg 361 tacgctacgc tgaggagcag caagacctgg ccctgctgtc catctccacc ttccaacgtg 421 gcctaaagga tcccaaccag ctgattcgtg ccagtgccct ccgtgtcctc tctagcatcc 481 gtgtgcccat catagtgccc atcatgatgc tagctatcaa ggaagccgcc tcggacatgt 541 caccctatgt gcggaaaaca gctgcccacg ccatccctaa actctacagt ttggactctg 601 accagaagga tcagctgata gaagtcattg agaagcttct ggctgacaag accacgctgg 661 tggcgggcag tgtggtgatg gcctttgagg aggtctgccc ggagcgcatc gacctgattc 721 acaaaaacta ccggaaactc tgtaacctgc tgatcgacgt ggaggagtgg ggccaggtgg 781 tcatcatcag catgctcacc cgctacgccc gcacgcagtt cctgagcccc acccagaacg 841 aatccctact agaggagaac gcggaaaaag ccttctacgg ctcagaggag gacgaggcca 901 agggcgcggg gtctgaggag acggccgccg cggccgcccc ctcccgaaag ccctatgtca 961 tggaccccga ccaccggctg ctgctgcgca acaccaaacc cctgctgcag agccgcagcg 1021 ccgcggtggt gatggcggtg gcgcagctct acttccacct ggcgcccaag gcggaagtgg 1081 gcgtcatcgc caaggcgctg gtgcgcctgc tgcgcagcca cagtgaggtg cagtacgttg 1141 tgctccagaa cgtggccacc atgtccatca agcgccgggg tatgtttgag ccctacctga 1201 agagcttcta catcaggtcc accgacccca cccagattaa gatcctgaag ctggaagtgc 1261 tgaccaacct ggccaatgag accaacattc ctactgtcct acgggaattc cagacctata 1321 ttcgcagcat ggacaaggac tttgtggcag ccacaatcca ggccattgga cgctgtgcaa 1381 ctaacatcgg ccgagtccgt gacacctgcc tcaatggcct ggtgcagctg ctgtccaacc 1441 gtgatgagct tgtggttgca gagtcagtgg tcgtcattaa gaaattgcta cagatgcagc 1501 cagcacaaca tggagagatc atcaaacact tggcaaagct tacagacaac atccaggtgc 1561 ccatggcccg agccagcatc ctgtggctca tcggagagta ctgtgagcat gtccccagga 1621 ttgcacctga tgtcttaaga aaaatggcca agtcattcac agcagaggag gatattgtca 1681 agctgcaggt catcaacctg gcagccaagc tctacctgac caactctaaa cagaccaagc 1741 tgctgaccca gtatgtgctg agtctggcca aatatgacca gaactatgat attcgcgacc 1801 gggcgcgctt cacccggcag ctcatcgtcc cttccgagca gggtggggcc ctcagccgcc 1861 atgccaagaa gctcttcctg gcacccaaac cagctccagt cttggagtca tccttcaaag 1921 accgggacca cttccagctg ggctcactgt cccacctgct taatgccaag gccacaggct 1981 accaggagct cccagactgg ccggaggaag ccccagaccc atctgtgcgc aacgtggagg 2041 tacctgaatg gaccaagtgc tcaaatcggg agaagagaaa ggagaaggaa aaacccttct 2101 actcggactc tgagggggag tcaggcccca cggagtccgc agacagtgac cctgagtctg 2161 agagtgaatc ggacagtaag agcagcagtg agagcggctc tggggagtcc agcagtgagt 2221 ccgacaatga agaccaggat gaggatgagg agaaagggag aggcagtgag agtgaacaga 2281 gtgaggagga tggtaagagg aagacaaaga agaaggtgcc agagagaaaa ggagaagcgt 2341 catcctctga tgagggcagc gattccagca gtagctcatc agagtccgag atgacatcgg 2401 agtccgagga ggagcagtta gaacctgcct cctggagcag gaaaacacct cccagcagca 2461 aaagtgctcc tgcaaccaag gagatctccc tgcttgatct agaggatttc acccctccca 2521 gtgtccagcc tgtgtctccc ccagcaattg tgtctaccag tctggctgct gacctggagg 2581 gcctgacact cacagactcc accctggtac cgtcgcttct gagtccagta tcgggtgttg 2641 ggctggcagg agctgctgca ccgggtagct ggcgaggggc tggctgtgga ctacaccttc 2701 agccgccaac ctttctccgg ggatccccac atggtgtccg tgcacatcca cttctccaac 2761 agctctgata cccccatcaa gggcctgcat gtgggcactc ccaaactgcc tgctggcatc 2821 agcatccaag aatttcccga aattgagtcc ctggcacctg gagaatctgc cactgctgta 2881 atgggcatta atttctgtga ctcaacccag gcagccaact tccagctgtg cacccagacc 2941 cgacagttcT ACGTCTCCAT TCAGCCACCT GTTGGGGAGC TGATGGCCCC TGTGTTCATG 3001 AGTGAAAATG AGTTTAAGAA GGAACAGGGA AAGCTGATGG GCATGAATGA GATCACAGAG 3061 AAACTCATGC TGCCAGACAC CTGTCGGAGT GACCACATTG TGGTGCAGAA AGTGACTGCC 3121 ACTGCCAACC TGGGTCGTGT TCCTTGTGGG ACATCTGATG AGTACAGGTT TGCAGGGAGG 3181 ACACTGACTG GTGGAAGCCT CGTTCTGCTG ACCCTGGATG CCCGGCCAGC TGGAGCTGCC 3241 CAGCTGACTG TCAACAGCGA GAAAATGGTG ATTGGCACCA TGCTGGTAAA GGATGTGATA 3301 CAGGCTCTGA CCCAGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 3361 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 3421 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTACTTGAA CTTTGCCACT 3481 TTCAGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt