Construct: ORF TRCN0000476441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007342.1_s317c1
- Derived from:
- ccsbBroadEn_04900
- DNA Barcode:
- CGAGTAACTTCAGACTCACCGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PM20D2 (135293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135293 | PM20D2 | peptidase M20 domain contai... | NM_001010853.3 | 100% | 100% | |
| 2 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XM_017010289.1 | 85.5% | 80.5% | (many diffs) |
| 3 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XM_005248661.2 | 57.3% | 57.3% | 0_1ins558 |
| 4 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XM_005248662.4 | 57.3% | 57.3% | 0_1ins558 |
| 5 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XM_011535481.3 | 57.3% | 57.3% | 0_1ins558 |
| 6 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XM_017010290.1 | 57.3% | 57.3% | 0_1ins558 |
| 7 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XM_017010291.1 | 57.3% | 57.3% | 0_1ins558 |
| 8 | human | 135293 | PM20D2 | peptidase M20 domain contai... | XR_001743167.1 | 24.2% | 1_132del;889_890ins155;1286_4590del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1377
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggcccgga ggggagcggc ccgtggaagg gggcgcgtgc aatggccgct 121 ccgagctgga gctactgaag ctgcgctcgg cggagtgcat cgacgaggcg gccgagcggc 181 tgggggccct gagccgcgcg atctggagcc agcccgagct ggcctacgag gagcaccatg 241 cccaccgcgt gctgacgcac ttcttcgagc gggagccgcc cgcggcctcc tgggcagtgc 301 agccgcacta ccagctgccc acggccttcc gcgccgagtg ggagccgccg gaggcccggg 361 caccgagcgc cacgccacgc ccgctgcacc tgggcttcct ctgcgagtac gacgcgctgc 421 ccggcatcgg ccacgcctgc ggccacaacc tcatcgctga ggtcggggcg gcggccgcgc 481 tgggcgtgag gggggcctta gagggcctcc ccaggccgcc tccgcccgtg aaggtagttg 541 tcctgggaac ccctgcagaa gaagatggtg gtggcaaaat tgatttaatt gaagcagggg 601 cttttacaaa tcttgatgtt gtttttatgg cccacccatc acaagagaat gctgcttatc 661 taccagatat ggctgaacat gatgtgactg tgaaatacta tggaaaagca tctcattctg 721 cttcttatcc ctgggaagga ttaaatgcat tagatgctgc tgtgctggcc tataacaatc 781 tgtctgtgtt cagacagcaa atgaaaccaa cctggagagt tcatggtata ataaaaaatg 841 gtggtgtaaa acccaatatc attccctctt attctgaatt aatctattac ttccgtgcac 901 cctcaatgaa agaacttcaa gttttgacca aaaaggcaga agattgcttc agagctgcag 961 ctttggcttc agggtgcaca gtggaaatta aaggtggagc acatgattat tacaatgttc 1021 ttcccaataa gagcctatgg aaagcctata tggaaaatgg aagaaagcta ggaatagagt 1081 tcatttcaga agatacaatg ttgaatggcc cttcaggatc tacggatttt ggaaatgtta 1141 gttttgtggt tcctggaaTT CATCCATATT TTCACATTGG ATCTAATGCC TTGAATCATA 1201 CTGAACAGTA CACTGAAGCT GCTGGGTCAC AGGAAGCTCA GTTCTACACT CTGCGGACGG 1261 CCAAAGCTCT GGCAATGACG GCACTGGATG TTATTTTTAA ACCAGAGTTA CTGGAAGGAA 1321 TCAGAGAGGA CTTTAAACTG AAACTTCAAG AAGAACAGTT TGTAAATGCA GTAGAATTGC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GACGAGTAAC TTCAGACTCA CCGTGCACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt