Construct: ORF TRCN0000476469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015961.1_s317c1
- Derived from:
- ccsbBroadEn_00763
- DNA Barcode:
- AGCACACTGACAGTATAACCGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNF4A (3172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_178850.3 | 100% | 100% | |
| 2 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001030004.3 | 92.6% | 91.8% | (many diffs) |
| 3 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001287184.2 | 92.5% | 91.4% | (many diffs) |
| 4 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_178849.3 | 86.4% | 79.7% | (many diffs) |
| 5 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_000457.4 | 84.7% | 79.5% | (many diffs) |
| 6 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001030003.3 | 79.9% | 72.7% | (many diffs) |
| 7 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001287182.2 | 79.8% | 72.4% | (many diffs) |
| 8 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001258355.2 | 78.4% | 71.1% | (many diffs) |
| 9 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_175914.4 | 78.4% | 72.5% | (many diffs) |
| 10 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | XM_005260407.4 | 78.3% | 73.6% | (many diffs) |
| 11 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001287183.1 | 78.3% | 72.3% | (many diffs) |
| 12 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | XM_006498787.1 | 76.4% | 78.7% | (many diffs) |
| 13 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | NM_008261.3 | 75.2% | 77.6% | (many diffs) |
| 14 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | NM_001312907.1 | 70.7% | 71.3% | (many diffs) |
| 15 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | XM_006498788.1 | 70.3% | 70.8% | (many diffs) |
| 16 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | NM_001312906.1 | 69.6% | 70.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1320
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgactctcc aaaaccctcg tcgacatgga catggccgac tacagtgctg 121 cactggaccc agcctacacc accctggaat ttgagaatgt gcaggtgttg acgatgggca 181 atgacacgtc cccatcagaa ggcaccaacc tcaacgcgcc caacagcctg ggtgtcagcg 241 ccctgtgtgc catctgcggg gaccgggcca cgggcaaaca ctacggtgcc tcgagctgtg 301 acggctgcaa gggcttcttc cggaggagcg tgcggaagaa ccacatgtac tcctgcagat 361 ttagccggca gtgcgtggtg gacaaagaca agaggaacca gtgccgctac tgcaggctca 421 agaaatgctt ccgggctggc atgaagaagg aagccgtcca gaatgagcgg gaccggatca 481 gcactcgaag gtcaagctat gaggacagca gcctgccctc catcaatgcg ctcctgcagg 541 cggaggtcct gtcccgacag atcacctccc ccgtctccgg gatcaacggc gacattcggg 601 cgaagaagat tgccagcatc gcagatgtgt gtgagtccat gaaggagcag ctgctggttc 661 tcgttgagtg ggccaagtac atcccagctt tctgcgagct ccccctggac gaccaggtgg 721 ccctgctcag agcccatgct ggcgagcacc tgctgctcgg agccaccaag agatccatgg 781 tgttcaagga cgtgctgctc ctaggcaatg actacattgt ccctcggcac tgcccggagc 841 tggcggagat gagccgggtg tccatacgca tccttgacga gctggtgctg cccttccagg 901 agctgcagat cgatgacaat gagtatgcct accTCAAAGC CATCATCTTC TTTGACCCAG 961 ATGCCAAGGG GCTGAGCGAT CCAGGGAAGA TCAAGCGGCT GCGTTCCCAG GTGCAGGTGA 1021 GCTTGGAGGA CTACATCAAC GACCGCCAGT ATGACTCGCG TGGCCGCTTT GGAGAGCTGC 1081 TGCTGCTGCT GCCCACCTTG CAGAGCATCA CCTGGCAGAT GATCGAGCAG ATCCAGTTCA 1141 TCAAGCTCTT CGGCATGGCC AAGATTGACA ACCTGTTGCA GGAGATGCTG CTGGGAGGTC 1201 CGTGCCAAGC CCAGGAGGGG CGGGGTTGGA GTGGGGACTC CCCAGGAGAC AGGCCTCACA 1261 CAGTGAGCTC ACCCCTCAGC TCCTTGGCTT CCCCACTGTG CCGCTTTGGG CAAGTTGCTT 1321 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAAGCAC ACTGACAGTA TAACCGGAGA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt