Construct: ORF TRCN0000476515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014592.1_s317c1
- Derived from:
- ccsbBroadEn_10085
- DNA Barcode:
- CCCCGTCATCGCAAGACTCACATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM189 (387521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387521 | TMEM189 | transmembrane protein 189 | NM_199129.3 | 99.5% | 98.8% | (many diffs) |
| 2 | human | 387521 | TMEM189 | transmembrane protein 189 | NM_001162505.1 | 98.3% | 97.7% | (many diffs) |
| 3 | human | 387522 | TMEM189-UBE2V1 | TMEM189-UBE2V1 readthrough | NM_199203.2 | 66.9% | 63.7% | (many diffs) |
| 4 | human | 387521 | TMEM189 | transmembrane protein 189 | NR_027889.1 | 33.5% | (many diffs) | |
| 5 | mouse | 407243 | Tmem189 | transmembrane protein 189 | NM_145538.2 | 86.9% | 91.8% | (many diffs) |
| 6 | mouse | 620966 | Gm6194 | predicted gene 6194; transm... | NR_033512.1 | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 879
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggcgcn gaggactggc cgggccagca gctggagctg gacgaggacg 121 aggcgtcttg ttgccgctgg ggcgcgcagc acgccggggc ccgcgagctg gctgcgctct 181 actcgccagg caagcgcctc caggagtggt gctctgtgat cctgtgcttc agcctcatcg 241 cccacaacct ggtccatctc ctgctgctgg cccgctggga ggacacaccc ctcgtcatac 301 tcggtgttgt tgcaggggct ctcattgctg acttcttgtc tggcctggta cactggggtg 361 ctgacacatg gggctctgtg gagctgccca ttgtggggaa ggctttcatc cgacccttcc 421 gggagcacaa cattgaccca acagctatca cacggcacga cttcatcgag accaacgggg 481 acaactgcct ggtgacactg ctgccgctgc taaacatggc ctacaagttc cgcacccaca 541 gccctgaagc cctGGAGCAG CTATACCACT GGGAGTGCTT CGTCTTCTGC CTGATCATCT 601 TCGGCACCTT CACCAACCAG ATCCACAAGT GGTCGCACAC GTACTTTGGG CTGCCACGCT 661 GGGTCACCCT CCTGCAGGAC TGGCATGTCA TCCTGCCACG TAAACACCAT CGCATCCACC 721 ACGTCTCACC CCACGAGACC TACTTCTGCA TCACCACAGG CTGGCTCAAC TACCCTCTGG 781 AGAAGATAGG CTTCTGGCGA CGCCTGGAGG ACCTCATCCA GGGCCTGACG GGCGAGAAGC 841 CTCGGGCAGA TGACATGAAA TGGGCCCAGA AGATCAAATT GCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGACCCCGT CATCGCAAGA CTCACATAAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt