Transcript: Human NM_199203.2

Homo sapiens TMEM189-UBE2V1 readthrough (TMEM189-UBE2V1), mRNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
TMEM189-UBE2V1 (387522)
Length:
2925
CDS:
162..1274

Additional Resources:

NCBI RefSeq record:
NM_199203.2
NBCI Gene record:
TMEM189-UBE2V1 (387522)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199203.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033707 AGGACAGTGTTACAGCAATTA pLKO.1 1253 CDS 100% 13.200 6.600 Y UBE2V1 n/a
2 TRCN0000426763 ATAGAATGTGGACCTAAATAC pLKO_005 1035 CDS 100% 13.200 6.600 Y TMEM189-UBE2V1 n/a
3 TRCN0000420546 CGATTTAATCAGTCTTCATTT pLKO_005 1316 3UTR 100% 13.200 6.600 Y TMEM189-UBE2V1 n/a
4 TRCN0000422529 TAAATTGATTCCCATCATAAC pLKO_005 1545 3UTR 100% 10.800 5.400 Y TMEM189-UBE2V1 n/a
5 TRCN0000033708 CGCCTAATGATGTCTAAAGAA pLKO.1 1206 CDS 100% 5.625 2.813 Y UBE2V1 n/a
6 TRCN0000033704 CCCTGGTTTCTTTAAGTCTTA pLKO.1 1788 3UTR 100% 4.950 2.475 Y UBE2V1 n/a
7 TRCN0000221042 CTCGTCATACTCGGTGTTGTT pLKO.1 384 CDS 100% 4.950 2.475 Y TMEM189 n/a
8 TRCN0000087485 GCTAAACATGGCCTACAAGTT pLKO.1 602 CDS 100% 4.950 2.475 Y Gm6194 n/a
9 TRCN0000221040 GCTGCTAAACATGGCCTACAA pLKO.1 599 CDS 100% 4.950 2.475 Y TMEM189 n/a
10 TRCN0000004031 ACCTACTTCTGCATCACCACA pLKO.1 831 CDS 100% 2.640 1.320 Y TMEM189-UBE2V1 n/a
11 TRCN0000033706 CCAAGAGCCATATCAGTGCTA pLKO.1 1134 CDS 100% 2.640 1.320 Y UBE2V1 n/a
12 TRCN0000004030 GCCACGTAAACACCATCGCAT pLKO.1 788 CDS 100% 2.640 1.320 Y TMEM189-UBE2V1 n/a
13 TRCN0000004029 CTTCGGCACCTTCACCAACCA pLKO.1 692 CDS 100% 0.880 0.440 Y TMEM189-UBE2V1 n/a
14 TRCN0000004032 CACACGTACTTTGGGCTGCCA pLKO.1 729 CDS 100% 0.220 0.110 Y TMEM189-UBE2V1 n/a
15 TRCN0000087483 CACGACTTCATCGAGACCAAT pLKO.1 549 CDS 100% 4.950 2.475 Y Gm6194 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199203.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10085 pDONR223 100% 66.9% 63.7% None (many diffs) n/a
2 ccsbBroad304_10085 pLX_304 0% 66.9% 63.7% V5 (many diffs) n/a
3 TRCN0000476515 CCCCGTCATCGCAAGACTCACATA pLX_317 40.3% 66.9% 63.7% V5 (many diffs) n/a
4 ccsbBroadEn_01741 pDONR223 100% 39.2% 38.1% None (many diffs) n/a
5 ccsbBroad304_01741 pLX_304 0% 39.2% 38.1% V5 (many diffs) n/a
6 TRCN0000468843 CTGAGCTAAAGGCTACTCTTGCGC pLX_317 82.6% 39.2% 38.1% V5 (many diffs) n/a
7 ccsbBroadEn_01742 pDONR223 100% 32.6% 34.8% None (many diffs) n/a
8 ccsbBroad304_01742 pLX_304 0% 32.6% 34.8% V5 (many diffs) n/a
9 TRCN0000474497 GGCACAGCCACCCTAATTTTCGTC pLX_317 87.6% 32.6% 34.8% V5 (many diffs) n/a
Download CSV