Transcript: Human NM_001162505.1

Homo sapiens transmembrane protein 189 (TMEM189), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
TMEM189 (387521)
Length:
2286
CDS:
162..965

Additional Resources:

NCBI RefSeq record:
NM_001162505.1
NBCI Gene record:
TMEM189 (387521)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001162505.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000344912 CAAATAACTTCTCCGAGCCTG pLKO_005 959 CDS 100% 2.160 3.024 N TMEM189 n/a
2 TRCN0000344858 ACCGAAGCCATCTGCCAAATT pLKO_005 1012 3UTR 100% 13.200 9.240 N TMEM189 n/a
3 TRCN0000221039 CTCGGGCAGATGACATGAAAT pLKO.1 925 CDS 100% 13.200 9.240 N TMEM189 n/a
4 TRCN0000333549 CTCGGGCAGATGACATGAAAT pLKO_005 925 CDS 100% 13.200 9.240 N TMEM189 n/a
5 TRCN0000221041 TGACATGAAATGGGCCCAGAA pLKO.1 935 CDS 100% 4.050 2.835 N TMEM189 n/a
6 TRCN0000221043 GCATCACCACAGGCTGGCTCA pLKO.1 832 CDS 100% 0.000 0.000 N TMEM189 n/a
7 TRCN0000333548 GCATCACCACAGGCTGGCTCA pLKO_005 832 CDS 100% 0.000 0.000 N TMEM189 n/a
8 TRCN0000344857 TACCCTCTGGAGAAGATAGGC pLKO_005 855 CDS 100% 0.000 0.000 N TMEM189 n/a
9 TRCN0000221042 CTCGTCATACTCGGTGTTGTT pLKO.1 384 CDS 100% 4.950 2.475 Y TMEM189 n/a
10 TRCN0000087485 GCTAAACATGGCCTACAAGTT pLKO.1 593 CDS 100% 4.950 2.475 Y Gm6194 n/a
11 TRCN0000221040 GCTGCTAAACATGGCCTACAA pLKO.1 590 CDS 100% 4.950 2.475 Y TMEM189 n/a
12 TRCN0000004031 ACCTACTTCTGCATCACCACA pLKO.1 822 CDS 100% 2.640 1.320 Y TMEM189-UBE2V1 n/a
13 TRCN0000004030 GCCACGTAAACACCATCGCAT pLKO.1 779 CDS 100% 2.640 1.320 Y TMEM189-UBE2V1 n/a
14 TRCN0000004029 CTTCGGCACCTTCACCAACCA pLKO.1 683 CDS 100% 0.880 0.440 Y TMEM189-UBE2V1 n/a
15 TRCN0000004032 CACACGTACTTTGGGCTGCCA pLKO.1 720 CDS 100% 0.220 0.110 Y TMEM189-UBE2V1 n/a
16 TRCN0000087483 CACGACTTCATCGAGACCAAT pLKO.1 540 CDS 100% 4.950 2.475 Y Gm6194 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001162505.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10085 pDONR223 100% 98.3% 97.7% None (many diffs) n/a
2 ccsbBroad304_10085 pLX_304 0% 98.3% 97.7% V5 (many diffs) n/a
3 TRCN0000476515 CCCCGTCATCGCAAGACTCACATA pLX_317 40.3% 98.3% 97.7% V5 (many diffs) n/a
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