Construct: ORF TRCN0000476527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009476.1_s317c1
- Derived from:
- ccsbBroadEn_02309
- DNA Barcode:
- ATCGGCACTGATTAGATTTTTAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UST (10090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10090 | UST | uronyl 2-sulfotransferase | NM_005715.3 | 100% | 100% | |
| 2 | human | 10090 | UST | uronyl 2-sulfotransferase | XR_001743088.2 | 57.1% | (many diffs) | |
| 3 | human | 10090 | UST | uronyl 2-sulfotransferase | XM_011535378.3 | 56.3% | 56.1% | (many diffs) |
| 4 | human | 10090 | UST | uronyl 2-sulfotransferase | XM_017010152.1 | 52.2% | 45.7% | (many diffs) |
| 5 | mouse | 338362 | Ust | uronyl-2-sulfotransferase | NM_177387.3 | 90.1% | 95.8% | (many diffs) |
| 6 | mouse | 338362 | Ust | uronyl-2-sulfotransferase | XM_006512772.3 | 50% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1287
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaagaag cagcagcatc ccggcggcgg cgcggatccc tggccccatg 121 gggcccctat ggggggcgcc cctccgggcc tgggcagctg gaagcgtcgg gtgcccctgc 181 tgcctttcct gcgcttctcc ctccgggact acggcttctg catggccacc ctgctggtct 241 tctgcctggg ctccctcctc tatcagctca gcgggggacc ccctcgcttc ctgctcgacc 301 tgcggcagta cttgggaaat tccacttact tggatgacca tggaccacct cctagtaagg 361 tactaccttt cccaagccag gtggtgtaca acagggtagg caagtgtggg agccgtactg 421 tggtcttgct tctgagaatc ttgtcggaga agcacggatt taatttggtc acatcagaca 481 ttcacaacaa aaccaggctt actaaaaatg aacaaatgga actgattaaa aatataagta 541 ctgccgaaca accctattta ttcactcgac atgttcattt cctcaacttc tcaaggtttg 601 gaggagacca gcctgtctac atcaacatca ttagagaccc cgtcaaccgg ttcttatcca 661 actatttttt ccgtcgcttt ggagactgga gaggggaaca aaatcacatg atccgcaccc 721 ccagcatgag gcaggaggag cgctacctgg atatcaatga gtgtattctt gaaaactatc 781 ccgagtgctc caaccccagg ttattttaca tcattccgta cttttgtgga cagcatccca 841 gatgcaggga gcctggtgaa tgggcccttg agagagcaaa gctgaacgtg aatgaaaact 901 tcctgctcgt ggggattctt gaagagttgg aagatgtgct gctgttactg gaaagatttt 961 tacctcatta cttcaagggc gtgctcagta tctacaaaga cccagagcac aggaagcttg 1021 gaaacatgac tgtgacggtg aagaagactg tcccctctcc tgaggctgtg cagatcctcT 1081 ACCAGCGGAT GAGATACGAG TACGAGTTTT ACCACTACGT CAAAGAGCAG TTCCACCTGC 1141 TGAAGCGCAA GTTTGGACTT AAGTCTCACG TCAGCAAGCC CCCCCTGAGG CCACACTTCT 1201 TTATCCCAAC TCCACTGGAA ACCGAGGAGC CAATCGACGA TGAAGAACAG GATGATGAAA 1261 AGTGGCTGGA AGATATTTAT AAGAGGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCGGCAC 1441 TGATTAGATT TTTAGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt