Construct: ORF TRCN0000476541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018465.1_s317c1
- Derived from:
- ccsbBroadEn_15934
- DNA Barcode:
- TCCAAACTTGTCGCGCACACCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM49 (57093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 642612 | TRIM49C | tripartite motif containing... | NM_001195234.1 | 100% | 100% | |
| 2 | human | 57093 | TRIM49 | tripartite motif containing 49 | NM_020358.2 | 99.6% | 99.3% | (many diffs) |
| 3 | human | 283116 | TRIM49B | tripartite motif containing... | NM_001206626.1 | 98% | 95.1% | (many diffs) |
| 4 | human | 729384 | TRIM49D2 | tripartite motif containing... | NM_001105522.1 | 91.7% | 84.7% | (many diffs) |
| 5 | human | 399939 | TRIM49D1 | tripartite motif containing... | NM_001206627.1 | 91.7% | 84.7% | (many diffs) |
| 6 | human | 399939 | TRIM49D1 | tripartite motif containing... | XM_011542816.3 | 91.7% | 84.7% | (many diffs) |
| 7 | human | 84767 | TRIM51 | tripartite motif-containing 51 | NM_032681.4 | 85.8% | 75% | (many diffs) |
| 8 | human | 642612 | TRIM49C | tripartite motif containing... | XM_017018126.1 | 82.9% | 82.9% | 505_506ins231 |
| 9 | human | 57093 | TRIM49 | tripartite motif containing 49 | XM_017018027.2 | 82.5% | 82.3% | (many diffs) |
| 10 | human | 57093 | TRIM49 | tripartite motif containing 49 | XM_024448617.1 | 82.4% | 74.5% | (many diffs) |
| 11 | human | 642612 | TRIM49C | tripartite motif containing... | XM_024448656.1 | 81.4% | 74.2% | (many diffs) |
| 12 | human | 283116 | TRIM49B | tripartite motif containing... | XM_017017561.1 | 81.1% | 78.5% | (many diffs) |
| 13 | human | 642569 | TRIM53AP | tripartite motif containing... | NR_028346.1 | 71.4% | (many diffs) | |
| 14 | human | 399940 | TRIM51EP | tripartite motif-containing... | NR_146921.1 | 68.6% | (many diffs) | |
| 15 | human | 79097 | TRIM48 | tripartite motif containing 48 | NM_024114.5 | 41.5% | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1425
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaattctgga atcttacagg tctttcaggg ggaactcatc tgccccctgt 121 gcatgaacta cttcatagac ccggtcacca tagactgtgg gcacagcttt tgcaggcctt 181 gtttctacct caactggcaa gacatcccat ttcttgtcca gtgctctgaa tgcacaaagt 241 caacagagca gataaacctc aaaaccaaca ttcatttgaa gaagatggct tctcttgcca 301 gaaaagtcag tctctggcta ttcctgagct ctgaggagca aatgtgtggc actcacaggg 361 agacaaagaa gatattctgt gaagtggaca ggagcctgct ctgtttgctg tgctccagct 421 ctcaggagca ccggtatcac agacaccgtc ccattgagtg ggctgctgag gaacaccggg 481 agaagctttt acagaaaatg cagtctttgt gggaaaaagc ttgtgaaaat cacagaaacc 541 tgaatgtgga aaccaccaga accagatgct ggaaggatta tgtgaattta aggctagaag 601 caattagagc tgagtatcag aagatgcctg catttcatca tgaagaagaa aaacataatt 661 tggagatgct gaaaaagaag gggaaagaaa tttttcatcg acttcattta agtaaagcca 721 aaatggctca taggatggag attttaagag gaatgtatga ggagctgaac gaaatgtgcc 781 ataaaccaga tgtggagcta cttcaggctt ttggagacat attacacagg agtgagtccg 841 tgctgctgca catgccccag cctctgaatc cagagctcag tgcagggccc atcactggac 901 tgagggacag gctcaaccaa ttccgagtgc atattactct gcatcatgaa gaagccaaca 961 gtgatatctt tctgtatgaa attttgagaa gcatgtgtat tggatgtgac catcaagatg 1021 taccctattt cactgcaaca cctagaagtt ttcttgcatg gggtgttcag actttcaccT 1081 CGGGCAAATA TTACTGGGAG GTCCATGTAG GGGACTCCTG GAATTGGGCT TTTGGTGTCT 1141 GTAATATGTA TCGGAAGGAG AAGAATCAGA ATGAGAAGAT AGATGGAAAG GAGGGACTCT 1201 TTCTTCTTGG GTGTATTAAG AATGACATTC AATGCAGTCT CTTTACCACC TCCCCACTTA 1261 TGCTGCAATA TATCCCAAAA CCTACCAGCC GAGTAGGATT ATTCCTGGAT TGTGAGGCTA 1321 AGACTGTGAG CTTTGTTGAT GTTAATCAAA GCTCCCTAAT ATACACCATC CCTAATTGCT 1381 CTTTCTCACC TCCTCTCAGG CCTATCTTTT GCTGTATTCA CTTCTTGCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA TCCAAACTTG TCGCGCACAC CGTTACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt