Construct: ORF TRCN0000476551

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004379.1_s317c1
Derived from:
ccsbBroadEn_07101
DNA Barcode:
CGGAATCACTATGTGAGCATTTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TSC2 (7249)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476551
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7249 TSC2 TSC complex subunit 2 NM_001114382.2 99.9% 99.7% (many diffs)
2 human 7249 TSC2 TSC complex subunit 2 NM_000548.5 98.6% 98.5% (many diffs)
3 human 7249 TSC2 TSC complex subunit 2 XM_017023615.1 98.5% 98.4% (many diffs)
4 human 7249 TSC2 TSC complex subunit 2 XM_011522636.2 97.6% 97.5% (many diffs)
5 human 7249 TSC2 TSC complex subunit 2 XM_011522637.2 97.6% 97.4% (many diffs)
6 human 7249 TSC2 TSC complex subunit 2 NM_001363528.1 97.5% 97.3% (many diffs)
7 human 7249 TSC2 TSC complex subunit 2 NM_001077183.2 97.4% 97.3% (many diffs)
8 human 7249 TSC2 TSC complex subunit 2 NM_001318832.1 96.8% 96.7% (many diffs)
9 human 7249 TSC2 TSC complex subunit 2 NM_021055.2 96.2% 96.1% (many diffs)
10 human 7249 TSC2 TSC complex subunit 2 NM_001370404.1 96.2% 96% (many diffs)
11 human 7249 TSC2 TSC complex subunit 2 NM_001370405.1 96% 95.9% (many diffs)
12 human 7249 TSC2 TSC complex subunit 2 XM_024450413.1 95.8% 95.7% (many diffs)
13 human 7249 TSC2 TSC complex subunit 2 NM_001318827.1 95.4% 95.2% (many diffs)
14 human 7249 TSC2 TSC complex subunit 2 XM_011522639.2 95.3% 95.1% (many diffs)
15 human 7249 TSC2 TSC complex subunit 2 XM_011522640.2 95.2% 95.1% (many diffs)
16 human 7249 TSC2 TSC complex subunit 2 NM_001318829.1 94.7% 94.6% (many diffs)
17 human 7249 TSC2 TSC complex subunit 2 XM_011522638.2 91.1% 90.9% (many diffs)
18 human 7249 TSC2 TSC complex subunit 2 XM_017023617.1 90.8% 90.6% (many diffs)
19 human 7249 TSC2 TSC complex subunit 2 NM_001318831.1 85.1% 85% (many diffs)
20 human 7249 TSC2 TSC complex subunit 2 XM_017023618.1 73.1% 73% (many diffs)
21 mouse 22084 Tsc2 tuberous sclerosis 2 NM_001286714.1 84.6% 91.1% (many diffs)
22 mouse 22084 Tsc2 tuberous sclerosis 2 NM_011647.3 84.6% 91.1% (many diffs)
23 mouse 22084 Tsc2 tuberous sclerosis 2 XM_006524040.2 84.6% 91.1% (many diffs)
24 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317387.1 84.5% 91.1% (many diffs)
25 mouse 22084 Tsc2 tuberous sclerosis 2 NM_001286713.1 83.5% 90% (many diffs)
26 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317384.1 83.5% 90% (many diffs)
27 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317386.1 83.5% 89.9% (many diffs)
28 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317385.1 83.5% 89.9% (many diffs)
29 mouse 22084 Tsc2 tuberous sclerosis 2 NM_001286720.1 82.5% 88% (many diffs)
30 mouse 22084 Tsc2 tuberous sclerosis 2 NM_001039363.2 82.3% 88.8% (many diffs)
31 mouse 22084 Tsc2 tuberous sclerosis 2 NM_001286716.1 82.3% 88.8% (many diffs)
32 mouse 22084 Tsc2 tuberous sclerosis 2 NM_001286718.1 82.3% 88.8% (many diffs)
33 mouse 22084 Tsc2 tuberous sclerosis 2 XM_006524044.2 82.3% 88.8% (many diffs)
34 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317392.1 82.3% 88.7% (many diffs)
35 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317388.1 81.3% 87.7% (many diffs)
36 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317389.1 81.3% 87.6% (many diffs)
37 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317390.1 80.6% 86.9% (many diffs)
38 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317391.1 80.6% 86.9% (many diffs)
39 mouse 22084 Tsc2 tuberous sclerosis 2 XR_001782062.1 79.8% (many diffs)
40 mouse 22084 Tsc2 tuberous sclerosis 2 XM_017317393.1 79.4% 85.8% (many diffs)
41 mouse 22084 Tsc2 tuberous sclerosis 2 XR_001782063.1 77.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
5421
ORF length:
5352
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggccaaacca acaagcaaag attcaggctt gaaggagaag tttaagattc
121tgttgggact gggaacaccg aggccaaatc ccaggtctgc agagggtaaa cagacggagt
181ttatcatcac cgcggaaata ctgagagaac tgagcatgga atgtggcctc aacaatcgca
241tccggatgat agggcagatt tgtgaagtcg caaaaaccaa gaaatttgaa gagcacgcag
301tggaagcact ctggaaggcg gtcgcggatc tgttgcagcc ggagcggccg ctggaggccc
361ggcacgcggt gctggctctg ctgaaggcca tcgtgcaggg gcagggcgag cgtttggggg
421tcctcagagc cctcttcttt aaggtcatca aggattaccc ttccaacgaa gaccttcacg
481aaaggctgga ggttttcaag gccctcacag acaatgggag acacatcacc tacttggagg
541aagagctggc tgactttgtc ctgcagtgga tggatgttgg cttgtcctcg gaattccttc
601tggtgctggt gaacttggtc aaattcaata gctgttacct cgacgagtac atcgcaagga
661tggttcagat gatctgtctg ctgtgcgtcc ggaccgtgtc ctctgtggac atagaggtct
721ccctgcaggt gctggacgcc gtggtctgct acaactgcct gccggctgag agcctcccgc
781tgttcatcgt taccctctgt cgcaccatca acgtcaagga gctctgcgag ccttgctgga
841agctgatgcg gaacctcctt ggcacccacc tgggccacag cgccatctac aacatgtgcc
901acctcatgga ggacagagcc tacatggagg acgcgcccct gctgagagga gccgtgtttt
961ttgtgggcat ggctctctgg ggagcccacc ggctctattc tctcaggaac tcgccgacat
1021ctgtgttgcc atcattttac caggccatgg catgtccgaa cgaggtggtg tcctatgaga
1081tcgtcctgtc catcaccagg ctcatcaaga agtataggaa ggagctccag gtggtggcgt
1141gggacattct gctgaacatc atcgaacggc tccttcagca gctccagacc ttggacagcc
1201cggagctcag gaccatcgtc catgacctgt tgaccacggt ggaggagctg tgtgaccaga
1261acgagttcca cgggtctcag gagagatact ttgaactggt ggagagatgt gcggaccaga
1321ggcctgagtc ctccctcctg aacctgatct cctatagagc gcagtccatc cacccggcca
1381aggacggctg gattcagaac ctgcaggcgc tgatggagag attcttcagg agcgagtccc
1441gaggcgccgt gcgcatcaag gtgctggacg tgctgtcctt tgtgctgctc atcaacaggc
1501agttctatga ggaggagctg attaactcag tggtcatctc gcagctctcc cacatccccg
1561aggataaaga ccaccaggtc cgaaagctgg ccacccagtt gctggtggac ctggcagagg
1621gctgccacac acaccacttc aacagcctgc tggacatcat cgagaaggtg atggcccgct
1681ccctctcccc acccccggag ctggaagaaa gggatgtggc cgcatactcg gcctccttgg
1741aggatgtgaa gacagccgtc ctggggcttc tggtcatcct tcagaccaag ctgtacaccc
1801tgcctgcaag ccacgccacg cgtgtgtatg agatgctggt cagccacatt cagctccact
1861acaagcacag ctacaccctg ccaatcgcga gcagcatccg gctgcaggcc tttgacttcc
1921tgttgctgct gcgggccgac tcactgcacc gcctgggcct gcccaacaag gatggagtcg
1981tgcggttcag cccctactgc gtctgcgact acatggagcc agagagaggc tctgagaaga
2041agaccagcgg ccccctttct cctcccacag ggcctcctgg cccggcgcct gcaggccccg
2101ccgtgcggct ggggtccgtg ccctactccc tgctcttccg cgtcctgctg cagtgcttga
2161agcaggagtc tgactgggag gtgctgaagc tggttctggg caggctgcct gagtccctgc
2221gctataaagt gctcatcttt acttcccctt gcagtgtgga ccagctgtgc tctgctctct
2281gctccatgct ttcaggccca aagacactgg agcggctccg aggcgcccca gaaggcttct
2341ccagaactga cttgcacctg gccgtggttc cagtgctgac agcattaatc tcttaccata
2401actacctgga caaaaccaaa cagcgcgaga tggtctactg cctggagcag ggcctcatcc
2461accgctgtgc cagccagtgc gtcgtggcct tgtccatctg cagcgtggag atgcctgaca
2521tcatcatcaa ggcgctgcct gttctggtgg tgaagctcac gcacatctca gccacagcca
2581gcatggccgt cccactgctg gagttcctgt ccactctggc caggctgccg cacctctaca
2641ggaactttgc cgcggagcag tatgccagtg tgttcgccat ctccctgccg tacaccaacc
2701cctccaagtt taatcagtac atcgtgtgtc tggcccatca cgtcatagcc atgtggttca
2761tcaggtgccg cctgcccttc cggaaggatt ttgtcccttt catcactaag ggcctgcggt
2821ccaatgtcct cttgtctttt gatgacaccc ccgagaagga cagcttcagg gcccggagta
2881ctagtctcaa cgagagaccc aagagtctga ggatagccag accccccaaa caaggcttga
2941ataactctcc acccgtgaaa gaattcaagg agagctctgc agccgaggcc ttccggtgcc
3001gcagcatcag tgtgtctgaa catgtggtcc gcagcaggat acagacgtcc ctcaccagtg
3061ccagcttggg gtctgcagat gagaactccg tggcccaggc tgacgatagc atgaaaaacc
3121tccacctgga gctcacggaa acctgtctgg acatgatggc tcgatacgtc ttctccaact
3181tcacggctgt cccgaagagg tctcctgtgg gcgagttcct cctagcgggt ggcaggacca
3241aaacctggct ggttgggaac aagcttgtca ctgtgacgac aagcgtggga accgggaccc
3301ggtcgttact aggcctggac tcgggggagc tgcagtccgg cccggagtcg agctccagcc
3361ccggggtgca tgtgagacag accaaggagg cgccggccaa gctggagtcc caggctgggc
3421agcaggtgtc ccgtggggcc cgggatcggg tccgttccat gtcggggggc catggtcttc
3481gagttggcgc cctggacgtg ccggcctccc agttcctggg cagtgccact tctccaggac
3541cacggactgc accagccgcg aaacctgaga aggcctcagc tggcacccgg gttcctgtgc
3601aggagaagac gaacctggcg gcctatgtgc ccctgctgac ccagggctgg gcggagatcc
3661tggtccggag gcccacaggg aacaccagct ggctgatgag cctggagaac ccgctcagcc
3721ctttctcctc ggacatcaac aacatgcccc tgcaggagct gtctaacgcc ctcatggcgg
3781ctgagcgctt caaggagcac cgggacacag ccctgtacaa gtcactgtcg gtgccggcag
3841ccagcacggc caaaccccct cctctgcctc gctccaacac agactccgcc gtggtcatgg
3901aggagggaag tccgggcgag gttcctgtgc tggtggagcc cccagggttg gaggacgttg
3961aggcagcgct aggcatggac aggcgcacgg atgcctacag caggtcgtcc tcagtctcca
4021gccaggagga gaagtcgctc cacgcggagg agctggttgg caggggcatc cccatcgagc
4081gagtcgtctc ctcggagggt ggccggccct ctgtggacct ctccttccag ccctcgcagc
4141ccctgagcaa gtccagctcc tctcccgagc tgcagactct gcaggacatc ctcggggacc
4201ctggggacaa ggccgacgtg ggccggctga gccctgaggt taaggcccgg tcacagtcag
4261ggaccctgga cggggaaagt gctgcctggt cggcctcggg cgaagacagt cggggccagc
4321ccgagggtcc cttgccttcc agctcccccc gctcgcccag tggcctccgg ccccgaggtt
4381acaccatctc cgactcggcc ccatcacgca ggggcaagag agtagagagg gacgccttaa
4441agagcagagc cacagcctcc aatgcagaga aagtgccagg catcaacccc agtttcgtgt
4501tcctgcagct ctaccattcc cccttctttg gcgacgagtc aaacaagcca atcctgctgc
4561ccaatgagtc acagtccttt gagcggtcgg tgcagctcct cgaccagatc ccatcatacg
4621acacccacaa gatcgccgtc ctgtatgttg gagaaggcca gagcaacagc gagctcgcca
4681tcctgtccaa tgagcatggc tcctacaggt acacggagtt cctgacgggc ctgggccggc
4741tcatcgagct gaaggactgc cagccggaca aggtgtacct gggaggcctg gacgtgtgtg
4801gtgaggacgg ccagttcacc tactgctggc acgatgacat catgcaagcc gtcttccaca
4861tcgccaccct gatgcccacc aaggacgtgg acaagcaccg ctgcgacaag aagcgccacc
4921tgggcaacga ctttgtgtcc attgtctaca atgactccgg tgaggacttc aagcttggca
4981ccatcaaggg ccagttcaac tttgtccacg tgatggtcac cccgctggac tacgagtgca
5041accTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA
5101AGATCGTGTC TGACCGCAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA
5161TGGCCTCACA GGTGCATCAT AGCCGCTCCA ACCCCACCGA TATCTACCCC TCCAAGTGGA
5221TTGCCCGGCT CCGCCACATC AAGCGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT
5281CCAACCCCAG CCTACCTCTG GTGCACCCTC CGTCCCATAG CAAAGCCCCT GCACAGACTC
5341CAGCCGAGCC CACACCTGGC TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG
5401AGGACTTCAC CGAGTTTGTG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
5461CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
5521AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGGA ATCACTATGT
5581GAGCATTTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)