Construct: ORF TRCN0000476553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012873.1_s317c1
- Derived from:
- ccsbBroadEn_08627
- DNA Barcode:
- ATCTAGCACTGAAGTGGAGTCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHAC2 (56134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476553
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56134 | PCDHAC2 | protocadherin alpha subfami... | NM_031883.2 | 99.8% | 100% | 117T>A;361T>C;702C>T |
| 2 | human | 56134 | PCDHAC2 | protocadherin alpha subfami... | NM_018899.6 | 86.4% | 84.3% | (many diffs) |
| 3 | mouse | 353237 | Pcdhac2 | protocadherin alpha subfami... | NM_001003672.1 | 75.8% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2721
- ORF length:
- 2652
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcaggcg ggcaccagac ctgcggcgac agagcatcca cggctccggc 121 ggcccatgcc ctggctgctg ctactgcctc tcctgctgct gttgctgctg ctgctacctg 181 gcccagcggc ctcccagctg cgatactctg tgccagagga gcaggcaccc ggcgcgctcg 241 tgggcaacgt ggctcgcgcg ctggggcttg agctgcggcg cttggggccg ggttgcttgc 301 gcatcaacca tctgggtgcg cccagtccgc gctacctgga gctggacctg acgagtggag 361 cgctcttcgt caacgagcgc attgatcggg aggcgctgtg tgagcagcgg cctcgctgcc 421 tgctcagcct ggaagtgctg gcgcacaacc ccgtggcggt gagcgccgtt gaggtggaaa 481 tattggacat caacgacaac tcaccgcgtt tcccgcggcc caactaccag cttcaggtaa 541 gcgaatcggt ggcgcctgga gcgcgctttc acatagagag tgcgcaggac cccgacgtgg 601 gcgccaactc agtacagacc tacgagctca gccccagcga gcacttcgag ctggacctta 661 agcccctgca ggagaacagt aaagtgcttg agctggtgct gcgtaagggc ctagaccggg 721 agcaggcagc cttgcaccac ctggttctca cagccgtgga tgggggcatt ccagcccgct 781 cgggtacggc acagatctct gtgcgtgtcc tggacactaa cgacaactct cctgcctttg 841 accagtccac ttatcgcgtc cagctacggg aggactcacc cccaggcaca ttggtggtga 901 agctgaatgc ctcagacccg gatgagggct ccaatggtga gctcaggtac tccttgagca 961 gctacacgtc ggaccgggag aggcagctct tcagcataga tgccagtacc ggggaagtgc 1021 gagtaattgg ggggctggat tatgaggaag cctcctccta ccagatctat gtgcaggcga 1081 ctgaccgggg tccagtgccc atggcaggtc actgcaaggt gctggtggac atcgtggacg 1141 tgaatgacaa tgccccagag gtggtgctca cggacctgta tagcccagtg cctgagaatg 1201 ctacacccaa caccattgtg gccgttctca gtgtcaatga ccaagactca ggccccaacc 1261 ggaaagtgag cctgggtctg gaggccacac tgcctttccg actgaatggc tttggaaact 1321 cctatacact ggtggtgagc ggcccactgg accgagagcg ggtggctgtc tacaacatca 1381 cggtgacagc cacagatggg ggaataccgc agctcacatc cctgcgtaca ctgaaggttg 1441 agatctctga catcaatgac aatccaccaa gcttcctgga ggactcctat tccatctaca 1501 tacaggagaa caatttgcca ggtgtgttgc tctgtactgt gcaagccaca gacccagatg 1561 aaaaggagaa tgcagaggtg acctactccc ttctggagag ggagattcaa gggctgccag 1621 tcacctccta tgtctccatt aacagtgcca gtggcagcct ttatgctgtc aactcctttg 1681 actatgagaa gtttcgggag ttctttgtga ctgtggaggc tcaggacaag gggagcccac 1741 cactgagcag cactgtgact gccaacgtat atgtggtgga catgaatgac catgcccctc 1801 acattctgta ccctacctca accaactcgt cagcagcctt cgagatggtg cctcgaactg 1861 cccctgctgg ctacctggtc accaaagtca tagctatgga ctcagactct gggcaaaatg 1921 cttggctttt ttaccatcta gcccagactt ctgacctgga cctctttaag gtagagctgc 1981 acacaggaga aattaggact accaggaaga tgggagatga gagtggtagc actttcaacc 2041 tgaccgtggt ggtccgagat aatggagagc catcactatc agcctctgtg gccattacag 2101 tagctgtggt ggatagggtt tccaaaatcc tccctgacac tcagaggcat gttaagagcc 2161 ctcggacata ctctgaaatt accctttatc taataatagc attaagcaca gtgtctttta 2221 tatttctttt gacaatcatc attttgagca tcatcaagtg ctaccgctac actgcgtatg 2281 gcactgcatg ctgtggaggc ttctgtggag taagggaaag gtcccctgca gaactgtaca 2341 aacaagccaa caacaatatt gatgccagga taccgcatgg cctcaaagtg cagcctcact 2401 tcattGAAGT TCGAGGGAAT GGCTCCCTCA CCAAGACCTA CTGCTACAAG GCCTGTCTGA 2461 CAGCAGGCTC AGGGAGTGAC ACTTTCATGT TTTACAATAC AGGGGCCCAG ACAGGACCAG 2521 GGCCTTCGGG AGCCCAAGCA GCAGTGACTG ACAGCAGGAA TCTCACAGGC CAAAGTGGTC 2581 AGAATGCTGG GAACCTGATT ATTCTCAAAA ATGAGGCTGT TTCTCAAAAT GAGGTGAGAC 2641 AGTGGTCAGG GGGTCTTCTA CAAACTCATG CATTTGTTAC ACATCCCCCA ATATCCTGTG 2701 ATTTGGCTTT ATTGAGTCAT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2761 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2821 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCT AGCACTGAAG 2881 TGGAGTCCTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt