Construct: ORF TRCN0000476555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001444.1_s317c1
- Derived from:
- ccsbBroadEn_06934
- DNA Barcode:
- ATCAGGAGGGATTTCTCCAGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFPQ (6421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6421 | SFPQ | splicing factor proline and... | NM_005066.3 | 99.8% | 99.4% | (many diffs) |
| 2 | human | 6421 | SFPQ | splicing factor proline and... | XM_005271112.5 | 99.8% | 99.4% | (many diffs) |
| 3 | human | 6421 | SFPQ | splicing factor proline and... | XM_005271113.5 | 99.8% | 99.4% | (many diffs) |
| 4 | human | 6421 | SFPQ | splicing factor proline and... | XM_017002053.2 | 99.8% | 99.4% | (many diffs) |
| 5 | human | 6421 | SFPQ | splicing factor proline and... | XM_017002054.2 | 99.8% | 99.4% | (many diffs) |
| 6 | human | 6421 | SFPQ | splicing factor proline and... | XM_011541950.2 | 94.7% | 91.2% | (many diffs) |
| 7 | human | 6421 | SFPQ | splicing factor proline and... | XM_005271115.4 | 93.8% | 93.2% | (many diffs) |
| 8 | human | 6421 | SFPQ | splicing factor proline and... | NR_136702.1 | 88.9% | (many diffs) | |
| 9 | human | 6421 | SFPQ | splicing factor proline and... | NR_136703.1 | 88.3% | (many diffs) | |
| 10 | mouse | 71514 | Sfpq | splicing factor proline/glu... | NM_023603.3 | 87.7% | 93.6% | (many diffs) |
| 11 | mouse | 71514 | Sfpq | splicing factor proline/glu... | NR_045010.1 | 78% | (many diffs) | |
| 12 | mouse | 71514 | Sfpq | splicing factor proline/glu... | XM_006503406.3 | 72.8% | 77.6% | (many diffs) |
| 13 | mouse | 71514 | Sfpq | splicing factor proline/glu... | XR_867891.2 | 30.9% | (many diffs) | |
| 14 | mouse | 71514 | Sfpq | splicing factor proline/glu... | XR_001784201.1 | 28.6% | (many diffs) | |
| 15 | mouse | 71514 | Sfpq | splicing factor proline/glu... | XR_867890.1 | 20.6% | (many diffs) | |
| 16 | mouse | 71514 | Sfpq | splicing factor proline/glu... | XR_001784200.1 | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcgggatcgg ttccggagtc gtggcggtgg cggtggtggc ttccacaggc 121 gtggaggagg cggcggccgc ggcggcctcc acgacttccg ttctccgccg cccggcatgg 181 gcctcaatca gaatcgcggc cccatgggtc ctggcccggg ccagagcggc cctaagcctc 241 cgatcccgcc accgcctcca caccaacagc agcaacagcc accaccgcag cagccaccgc 301 cgcagcagcc gccaccgcat cagccgccgc cgcatccaca gccgcatcag cagcagcagc 361 cgccgccacc gccgcaggac tcttccaagc ccgtcgttgc tcagggaccc ggccccgctc 421 ccggagtagg cagcacacca ccagcctcca gctcggcccc gcccgccact ccaccaacct 481 cgggggcccc gccagggtcc gggccaggcc cgactccgac cccgccgcct gcagtcacct 541 cggcccctcc cggggcgccg ccacccaccc cgccaagcag cggggtccct accacacctc 601 ctcaggccgg aggcccgccg cctccgcccg cggcagtccc gggcccgggt ccagggccta 661 agcagggccc aggtccgggt ggtcccaaag gcggcaaaat gcctggcggg ccgaagccag 721 gtggcggccc gggcctaagt acgcctggcg gccaccccaa gccgccgcgt cgaggcggcg 781 gggagccccg cgggggccgc cagcaccacc cgccctacca ccagcagcat caccaggggc 841 ccccgcccgg cgggcccggc ggccgcagcg aggagaagat ctcggactcg gaggggttta 901 aagccaattt gtctctcttg aggaggcctg gagagaaaac ttacacacag cgatgtcggt 961 tgtttgttgg gaatctacct gctgatatca cggaggatga attcaaaaga ctatttgcta 1021 aatatggaga accaggagaa gtttttatca acaaaggcaa aggattcgga tttattaagc 1081 ttgaatctag agctttggct gaaattgcca aagccgaact ggatgataca cccatgagag 1141 gtagacagct tcgagttcgc tttgccacac atgctgctgc cctttctgtt cgtaatcttt 1201 caccttatgt ttccaatgaa ctgttggaag aagcctttag ccaatttggt cctattgaaa 1261 gggctgttgt aatagtggat gatcgtggaa gatctacagg gaaaggcatt gttgaatttg 1321 cttctaagcc agcagcaaga aaggcatttg aacgatgcag tgaaggtgtt ttcttactga 1381 cgacaactcc tcgtccagtc attgtggaac cacttgaaca actagatgat gaagatggtc 1441 ttcctgaaaa acttgcccag aagaatccaa tgtatcaaaa ggagagagaa acccctactc 1501 gttttgccca gcatggcacg tttgagtacg aatattctca gcgatggaag tctttggatg 1561 aaatggaaaa acagcaaagg gaacaagttg aaaaaaacat gaaagatgca aaagacaaat 1621 tggaaagtga aatggaagat gcctatcatg aacatcaggc aaatcttttg cgccaagatc 1681 tgatgagacg acaggaagaa ttaagacgca tggaagaact tcacaatcaa gaaatgcaga 1741 aacgtaaaga aatgcaattg aggcaagagg aggaacgacg tagaagagag gaagagatga 1801 tgattcgtca acgtgagatg gaagaccaaa TGAGGCGCCA AAGAGAGGAA AGTTACAGCC 1861 GAATGGGCTA CATGGATCCA CGGGAAAGAG ACATGCGAAT GGGTGGCGGA GGAGCAATGA 1921 ACATGGGAGA TCCCTATGGT TCAGGAGGCC AGAAATTTCC ACCTCTAGGA GGTGGTGGTG 1981 GCATAGGTTA TGAAGCTAAT CCTGGCGTTC CACCAGCAAC CATGAGTGGT TCCATGATGG 2041 GAAGTGACAT GCGTACTGAG CGCTTTGGGC AGGGAGGTGC GGGGCCTGTG GGTGGACAGG 2101 GTCCTAGAGG AATGGGGCCT GGAACTCCAG CAGGATATGG TAGAGGGAGA GAAGAGTACG 2161 AAGGCCCAAA CAAAAAACCC CGATTTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCAGGAG 2341 GGATTTCTCC AGACCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt