Construct: ORF TRCN0000476702
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004388.1_s317c1
- Derived from:
- ccsbBroadEn_01097
- DNA Barcode:
- TCCATCAACGTTTCGGACATTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NHLH2 (4808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476702
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4808 | NHLH2 | nescient helix-loop-helix 2 | NM_001111061.2 | 100% | 100% | |
| 2 | human | 4808 | NHLH2 | nescient helix-loop-helix 2 | NM_005599.3 | 100% | 100% | |
| 3 | human | 4808 | NHLH2 | nescient helix-loop-helix 2 | XR_946659.3 | 8.3% | 1_516del;922_4869del | |
| 4 | mouse | 18072 | Nhlh2 | nescient helix loop helix 2 | NM_178777.3 | 91.8% | 97.7% | (many diffs) |
| 5 | mouse | 18072 | Nhlh2 | nescient helix loop helix 2 | XM_006501112.2 | 91.8% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 474
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggCACCAT GATGCTGAGT CCGGACCAAG CAGCAGATTC GGACCATCCC AGCTCGGCGC 121 ACTCGGATCC GGAGTCCCTG GGCGGCACGG ACACCAAGGT GCTCGGCAGC GTGTCGGACC 181 TGGAGCCGGT GGAGGAGGCC GAGGGCGACG GCAAGGGCGG CAGCCGAGCC GCGCTCTACC 241 CGCACCCGCA GCAGCTGAGC CGCGAGGAGA AGCGCCGCCG CCGGCGCGCC ACGGCCAAGT 301 ACCGCTCGGC CCACGCCACC CGCGAGCGCA TCCGCGTGGA AGCCTTCAAC TTGGCCTTCG 361 CCGAGCTCCG CAAATTGCTG CCCACGCTGC CCCCGGACAA GAAGCTCTCC AAGATCGAGA 421 TCCTGCGCCT GGCCATCTGC TACATCTCCT ATCTCAACCA CGTCCTGGAC GTGTTGCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAT CCATCAACGT TTCGGACATT GGTACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att