Construct: ORF TRCN0000476792

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017155.5_s317c1
Derived from:
ccsbBroadEn_05768
DNA Barcode:
GCATCAATGGAAATCAAAGACCTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACVR2A (92)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476792
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92 ACVR2A activin A receptor type 2A NM_001278579.2 99.9% 100% 354G>A
2 human 92 ACVR2A activin A receptor type 2A NM_001616.5 99.9% 100% 354G>A
3 human 92 ACVR2A activin A receptor type 2A NM_001278580.1 78.8% 78.9% 0_1ins324;30G>A
4 mouse 11480 Acvr2a activin receptor IIA NM_007396.4 94.5% 99.6% (many diffs)
5 mouse 11480 Acvr2a activin receptor IIA XM_006497623.3 93% 98% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1605
ORF length:
1539
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg agctgctgca aagttggcgt ttgccgtctt tcttatctcc tgttcttcag
121gtgctatact tggtagatca gaaactcagg agtgtctttt ctttaatgct aattgggaaa
181aagacagaac caatcaaact ggtgttgaac cgtgttatgg tgacaaagat aaacggcggc
241attgttttgc tacctggaag aatatttctg gttccattga aatagtgaaa caaggttgtt
301ggctggatga tatcaactgc tatgacagga ctgattgtgt agaaaaaaaa gacagccctg
361aagtatattt ttgttgctgt gagggcaata tgtgtaatga aaagttttct tattttccag
421agatggaagt cacacagccc acttcaaatc cagttacacc taagccaccc tattacaaca
481tcctgctcta ttccttggtg ccacttatgt taattgcggg gattgtcatt tgtgcatttt
541gggtgtacag gcatcacaag atggcctacc ctcctgtact tgttccaact caagacccag
601gaccaccccc accttctcca ttactaggtt tgaaaccact gcagttatta gaagtgaaag
661caaggggaag atttggttgt gtctggaaag cccagttgct taacgaatat gtggctgtca
721aaatatttcc aatacaggac aaacagtcat ggcaaaatga atacgaagtc tacagtttgc
781ctggaatgaa gcatgagaac atattacagt tcattggtgc agaaaaacga ggcaccagtg
841ttgatgtgga tctttggctg atcacagcat ttcatgaaaa gggttcacta tcagactttc
901ttaaggctaa tgtggtctct tggaatgaac tgtgtcatat tgcagaaacc atggctagag
961gattggcata tttacatgag gatatacctg gcctaaaaga tggccacaaa cctgccatat
1021ctcacaggga catcaaaagt aaaaatgtgc tgttgaaaaa caacctgaca gcttgcattg
1081ctgactttgg gttggCCTTA AAATTTGAGG CTGGCAAGTC TGCAGGCGAT ACCCATGGAC
1141AGGTTGGTAC CCGGAGGTAC ATGGCTCCAG AGGTATTAGA GGGTGCTATA AACTTCCAAA
1201GGGATGCATT TTTGAGGATA GATATGTATG CCATGGGATT AGTCCTATGG GAACTGGCTT
1261CTCGCTGTAC TGCTGCAGAT GGACCTGTAG ATGAATACAT GTTGCCATTT GAGGAGGAAA
1321TTGGCCAGCA TCCATCTCTT GAAGACATGC AGGAAGTTGT TGTGCATAAA AAAAAGAGGC
1381CTGTTTTAAG AGATTATTGG CAGAAACATG CTGGAATGGC AATGCTCTGT GAAACCATTG
1441AAGAATGTTG GGATCACGAC GCAGAAGCCA GGTTATCAGC TGGATGTGTA GGTGAAAGAA
1501TTACCCAGAT GCAGAGACTA ACAAATATTA TTACCACAGA GGACATTGTA ACAGTGGTCA
1561CAATGGTGAC AAATGTTGAC TTTCCTCCCA AAGAATCTAG TCTATGCCCA ACTTTCTTGT
1621ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1681AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1741GAAAGGACGA GCATCAATGG AAATCAAAGA CCTGACGCGT TAAGTCgaca atcaacctct
1801ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)