Construct: ORF TRCN0000476819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011113.1_s317c1
- Derived from:
- ccsbBroadEn_13485
- DNA Barcode:
- AGTCCACTTCATTGGAAGCTGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NALCN (259232)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350749.2 | 37.1% | 33.2% | (many diffs) |
| 2 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_052867.4 | 37.1% | 33.2% | (many diffs) |
| 3 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_011521067.2 | 36.7% | 32.8% | (many diffs) |
| 4 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350748.2 | 36.5% | 32.6% | (many diffs) |
| 5 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_024449336.1 | 36.1% | 32.3% | (many diffs) |
| 6 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350750.2 | 35.5% | 31.6% | (many diffs) |
| 7 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350751.2 | 35.5% | 31.6% | (many diffs) |
| 8 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_011521069.2 | 35.1% | 31.2% | (many diffs) |
| 9 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_017020536.2 | 27.9% | 21.5% | (many diffs) |
| 10 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_017020537.1 | 22.4% | 18.4% | (many diffs) |
| 11 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316066.2 | 47.4% | 46.7% | (many diffs) |
| 12 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | NM_177393.4 | 33.1% | 33% | (many diffs) |
| 13 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316064.2 | 32.8% | 32.7% | (many diffs) |
| 14 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_030247866.1 | 32.6% | 34.6% | (many diffs) |
| 15 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_011245094.3 | 32.5% | 32.4% | (many diffs) |
| 16 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316063.2 | 32.3% | 34.3% | (many diffs) |
| 17 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316062.2 | 32.2% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2070
- ORF length:
- 2004
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct caaaaggaag cagagttcca gggtggaagc ccagccagtc actgactttg 121 gtcctgatga gtctctgtcg gataatgctg acatcctctg gattaacaaa ccatgggttc 181 actctttgct gcgcatctgt gccatcatca gcgtcatttc tgtttgtatg aatacgccaa 241 tgaccttcga gcactatcct ccacttcagt atgtgacctt cactttggat acattattga 301 tgtttctcta cacggcagag atgatagcaa aaatgcacat ccggggcatt gtcaaggggg 361 atagttccta tgtgaaagat cgctggtgtg tttttgatgg atttatggtc ttttgccttt 421 gggtttcttt ggtgctacag gtgtttgaaa ttgctgatat agttgatcag atgtcacctt 481 ggggcatgtt gcggattcca cggccactga ttatgatccg agcattccgg atttatttcc 541 gatttgaact gccaaggacc agaattacaa atattttaaa gcgatcggga gaacaaatat 601 ggagtgtttc catttttcta cttttctttc tacttcttta tggaatttta ggagttcaga 661 tgtttggaac atttacttat cactgtgttg taaatgacac aaagccaggg aatgtaacct 721 ggaatagttt agctattcca gacacacact gctcaccaga gctagaagaa ggctaccagt 781 gcccacctgg atttaaatgc atggaccttg aagatctggg acttagcagg caagagctgg 841 gctacagtgg ctttaatgag ataggaacta gtatattcac cgtctatgag gccgcctcac 901 aggaaggctg ggtgttcctc atgtacagag caattgacag ctttccccgt tggcgttcct 961 acttctattt catcactctc attttcttcc tcgcctggct tgtgaagaac gtgtttattg 1021 ctgttatcat tgaaacattt gcagaaatca gagtacagtt tcaacaaatg tggggatcga 1081 gaagcagcac tacctcaaca gccaccaccc agatgtttca tgaagatgct gctggaggtt 1141 ggcagctggt agccgtggat gtcaacaagc cccagggacg cgccccagcc tgcctccaga 1201 aaatgatgcg gtcatccgtt ttccacatgt tcatcctgag catggtgacc gtggacgtga 1261 tcgtggcggc tagcaactac tacaaaggag aaaacttcag gaggcagtac gacgagttct 1321 acctggcgga ggtggctttt acagtacttt ttgatttgga agcacttctg aagatatggt 1381 gtttgggatt tactggatat attagctcat ctctccacaa attcgaacta ctactcgtaa 1441 ttggaactac tcttcatgta tacccagatc tttatcattc acaattcacg tactttcagg 1501 ttctccgagt agttcggctg attaagattt cacctgcatt agaagacttt gtgtacaaga 1561 tatttggtcc tggaaaaaag cttgggagtt tggttgtatt tactgccagc ctcttgattg 1621 ttatgtcagc aattagtttg cagatgttct gctttgttga agaactggac agatttacta 1681 cgtttccgag ggcatttatg tccatgttcc agaTCCTCAC CCAGGAAGGA TGGGTGGACG 1741 TAATGGACCA AACTCTAAAT GCTGTGGGAC ATATGTGGGC ACCCGTGGTT GCCATCTATT 1801 TCATTCTCTA TCATCTTTTT GCCACTCTGC CTCCCTCTCT TATCCGTGAC CTCTGTGGCA 1861 CTCAAGACGC CTGTCCATCC TGCCTTCCTC TCCAGCCTCC AAACCATCTG CCTGGAAGCC 1921 AGACTCTGGC CAGACTTACT CACCAGGCCC TCACCACACC ACCTGGAATG CGTTCCTCAC 1981 AGCTCTTTTC CAAAATCAGT CTTCTCCTTG GCATCTGCGT GGCTTGGGAG AGCATCCTTG 2041 GCTCTCTGTC ATTGTCTCAC AATCTTCAAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2101 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2161 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGTCC 2221 ACTTCATTGG AAGCTGTTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2281 tgaaagatt