Construct: ORF TRCN0000476867

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002925.1_s317c1
Derived from:
ccsbBroadEn_13899
DNA Barcode:
ATTCCTAATTTTTGTCTTACCCCC
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MPP3 (4356)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476867
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4356 MPP3 membrane palmitoylated prot... NM_001932.5 99.9% 99.4% 1746delT
2 human 4356 MPP3 membrane palmitoylated prot... XM_006721915.2 99.9% 99.4% 1746delT
3 human 4356 MPP3 membrane palmitoylated prot... NM_001353080.1 98.5% 98.1% 223_246del;1770delT
4 human 4356 MPP3 membrane palmitoylated prot... NM_001330233.1 95.8% 95.4% 1_75del;1821delT
5 human 4356 MPP3 membrane palmitoylated prot... XR_001752516.1 76% (many diffs)
6 human 4356 MPP3 membrane palmitoylated prot... XR_002958011.1 75.9% (many diffs)
7 human 4356 MPP3 membrane palmitoylated prot... XR_001752517.2 72% (many diffs)
8 human 4356 MPP3 membrane palmitoylated prot... XR_934466.2 71.8% (many diffs)
9 human 4356 MPP3 membrane palmitoylated prot... XR_001752512.2 71.6% (many diffs)
10 human 4356 MPP3 membrane palmitoylated prot... XM_017024656.1 65.3% 64.7% 1_1delAins607;1140delT
11 human 4356 MPP3 membrane palmitoylated prot... XM_017024657.2 60.6% 60.1% 0_1ins690;1056delT
12 human 4356 MPP3 membrane palmitoylated prot... NR_003562.3 60.4% (many diffs)
13 human 4356 MPP3 membrane palmitoylated prot... XR_001752515.1 59.5% 1_339del;1348_1629del;2035_2036ins340
14 human 4356 MPP3 membrane palmitoylated prot... NR_148343.1 59% (many diffs)
15 human 4356 MPP3 membrane palmitoylated prot... NR_148342.1 56% (many diffs)
16 human 4356 MPP3 membrane palmitoylated prot... NR_148344.1 54.3% (many diffs)
17 human 4356 MPP3 membrane palmitoylated prot... NR_148345.1 53.8% (many diffs)
18 human 4356 MPP3 membrane palmitoylated prot... XM_017024658.1 51.6% 51.6% 1_75del;1020_1021ins809
19 human 4356 MPP3 membrane palmitoylated prot... XM_006721916.3 48.5% 39.7% (many diffs)
20 human 4356 MPP3 membrane palmitoylated prot... XM_017024659.1 48.4% 48.3% (many diffs)
21 human 4356 MPP3 membrane palmitoylated prot... XM_017024655.1 48.2% 39.2% 1_75del;683_815del;1155_1156ins807
22 human 4356 MPP3 membrane palmitoylated prot... XM_017024660.1 35% 35% (many diffs)
23 mouse 13384 Mpp3 membrane protein, palmitoyl... NM_007863.2 87.3% 90.4% (many diffs)
24 mouse 13384 Mpp3 membrane protein, palmitoyl... XM_006532138.3 87.3% 90.4% (many diffs)
25 mouse 13384 Mpp3 membrane protein, palmitoyl... XM_006532139.3 77% 78.9% (many diffs)
26 mouse 13384 Mpp3 membrane protein, palmitoyl... XM_006532140.3 47.7% 51.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1821
ORF length:
1755
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc agtgctatcg gaggactctg gtttgcatga aaccctggcc ctgctgacct
121cccagctcag acctgactcc aaccacaagg aggagatggg cttcctgagg gatgttttca
181gtgaaaaaag cctcagttac ttaatgaaga ttcatgagaa gcttcgctat tatgaaaggc
241aaagtccaac cccagttctg cacagcgctg tggccctcgc tgaggacgtg atggaggagt
301tgcaggccgc ctccgtgcac agtgatgaga gggagctgct ccagctgctg tccaccccgc
361acctgagggc tgtgctcatg gtacatgaca cggttgccca gaagaatttt gaccccgttc
421tcccgcctct gcctgacaat atcgatgagg attttgatga ggaatcggtg aagatcgtcc
481gcttggtgaa gaacaaggaa cccctgggtg ccaccatccg gcgggacgag cactcagggg
541ctgttgtggt ggccaggatc atgcgaggag gcgcagcaga caggagcggc ctggtccacg
601ttggagatga gctccgagaa gtgaacggga tcgcagtcct gcacaagcgg cccgacgaga
661tcagccagat tctggcccag tcccagggat ccatcaccct aaaaatcatc ccagccaccc
721aggaggaaga tcgcttaaag gagagcaagg tgttcatgcg cgccctcttc cactacaacc
781ctcgggagga ccgggccatc ccttgccagg aggcgggcct gcccttccag cgcaggcagg
841tcctggaggt ggtgagccag gacgacccca cgtggtggca ggccaagcga gtcggggaca
901ccaaccttcg agccggcctc atcccctcca aggggttcca ggagagacga ctaagctacc
961ggagagccgc gggcaccctg ccgagccccc agagcctcag gaagcccccc tatgatcagc
1021cttgtgacaa agagacctgt gactgtgagg gctacctcaa agggcactat gtggctggtc
1081ttcggaggag cttccggctg ggctgtaggg agagactggg tggctcgcag gaaggaaaga
1141tgtcctccgg agctgagtct ccggagctgc tgacttacga agaggtggcc aggtaccaac
1201accagcccgg agagcggccc cgcctggtgg ttctgatcgg gtctctggga gcccgactgc
1261acgagctgaa gcaaaaggtg gtggctgaga acccacagca ctttggcgtc gctgttccac
1321ataccaccag gccccgaaag agccatgaga aggaaggagt ggaatatcac tttgtgtcta
1381agcaagcatt tgaggccgac ttacatcaca acaagttCCT GGAACATGGT GAATATAAGG
1441AAAATCTGTA TGGAACCAGC CTGGAGGCCA TTCAGGCTGT TATGGCCAAA AACAAAGTTT
1501GTTTGGTGGA TGTGGAGCCA GAAGCACTGA AACAACTGAG GACCTCAGAA TTTAAACCCT
1561ATATTATATT TGTAAAGCCT GCAATTCAGG AAAAAAGAAA AACGCCACCT ATGTCCCCAG
1621CTTGTGAGGA CACAGCAGCC CCATTTGATG AGCAGCAGCA AGAGATGGCC GCTTCTGCCG
1681CCTTCATAGA CCGGCATTAC GGGCACCTGG TAGACGCCGT GCTGGTGAAG GAGGATCTCC
1741AGGGTGCCTA CAGCCAGCTC AAAGTGGTCT TAGAGAAGCT GAGCAAGGAC ACTCACTGGG
1801TACCTGTTAG TGGGTCAGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC
1861TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA
1921AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTCC TAATTTTTGT
1981CTTACCCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)