Transcript: Human XM_006721916.3

PREDICTED: Homo sapiens membrane palmitoylated protein 3 (MPP3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MPP3 (4356)
Length:
1396
CDS:
184..1347

Additional Resources:

NCBI RefSeq record:
XM_006721916.3
NBCI Gene record:
MPP3 (4356)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006721916.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006134 CCTGACAATATCGATGAGGAT pLKO.1 625 CDS 100% 2.640 3.696 N MPP3 n/a
2 TRCN0000356501 GTTCCAGGAGAGACGACTAAG pLKO_005 1261 CDS 100% 10.800 8.640 N MPP3 n/a
3 TRCN0000356504 CATGAGAAGCTTCGCTATTAT pLKO_005 406 CDS 100% 15.000 10.500 N MPP3 n/a
4 TRCN0000235629 CAGGAGGAAGATCGCTTAAAG pLKO_005 1046 CDS 100% 13.200 9.240 N MPP3 n/a
5 TRCN0000006132 CCTCAGTTACTTAATGAAGAT pLKO.1 384 CDS 100% 4.950 3.465 N MPP3 n/a
6 TRCN0000356575 CCAGGGATCCATCACCCTAAA pLKO_005 1009 CDS 100% 10.800 6.480 N MPP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006721916.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13899 pDONR223 100% 48.5% 39.7% None (many diffs) n/a
2 ccsbBroad304_13899 pLX_304 0% 48.5% 39.7% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000476867 ATTCCTAATTTTTGTCTTACCCCC pLX_317 23.2% 48.5% 39.7% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_14702 pDONR223 92.8% 48.5% 59.4% None (many diffs) n/a
5 ccsbBroad304_14702 pLX_304 0% 48.5% 59.4% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000479442 CTCCCCGACTCACATGTCCGATAG pLX_317 16.2% 48.5% 59.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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