Construct: ORF TRCN0000476870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005246.1_s317c1
- Derived from:
- ccsbBroadEn_00286
- DNA Barcode:
- CCTTATTCGGCCTCCTTTGTAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDS1 (1040)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1040 | CDS1 | CDP-diacylglycerol synthase 1 | NM_001263.4 | 100% | 100% | |
| 2 | human | 1040 | CDS1 | CDP-diacylglycerol synthase 1 | XM_017007648.2 | 95% | 95% | 810_811ins69 |
| 3 | human | 1040 | CDS1 | CDP-diacylglycerol synthase 1 | XM_005262687.3 | 91.3% | 91.3% | 1030_1031ins120 |
| 4 | human | 1040 | CDS1 | CDP-diacylglycerol synthase 1 | XM_017007649.2 | 86.3% | 86.3% | 810_811ins69;961_962ins120 |
| 5 | human | 1040 | CDS1 | CDP-diacylglycerol synthase 1 | XM_017007650.2 | 78% | 75.4% | 1030_1031ins224;1080_1081ins79 |
| 6 | human | 1040 | CDS1 | CDP-diacylglycerol synthase 1 | XM_017007651.2 | 73.1% | 70.4% | 810_811ins69;961_962ins224;1011_1012ins79 |
| 7 | mouse | 74596 | Cds1 | CDP-diacylglycerol synthase 1 | NM_173370.3 | 86.6% | 95.8% | (many diffs) |
| 8 | mouse | 74596 | Cds1 | CDP-diacylglycerol synthase 1 | XM_006535263.2 | 75.1% | 83.5% | (many diffs) |
| 9 | mouse | 74596 | Cds1 | CDP-diacylglycerol synthase 1 | XM_006535264.1 | 70.6% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1452
- ORF length:
- 1383
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggagctg aggcaccggg gaagctgccc cggccccagg gaagcggtgt 121 cgccgccaca ccgcgaggga gaggcggccg gcggcgacca cgaaaccgag agcaccagcg 181 acaaagaaac agatattgat gacagatatg gagatttgga ttccagaaca gattctgata 241 ttccggaaat tccaccatcc tcagatagaa cccctgagat tctcaaaaaa gctctatctg 301 gtttatcttc aaggtggaaa aactggtgga tacgtggaat tctcactcta actatgatct 361 cgttgttttt cctgatcatc tatatgggat ccttcatgct gatgcttctt gttctgggca 421 tccaagtgaa atgcttccat gaaattatca ctataggtta tagagtctat cattcttatg 481 atctaccatg gtttagaaca ctaagttggt actttctatt gtgtgtaaac tactttttct 541 atggagagac tgtagctgat tattttgcta catttgttca aagagaagaa caacttcagt 601 tcctcattcg ctaccataga tttatatcat ttgccctcta tctggcaggt ttctgcatgt 661 ttgtactgag tttggtgaag aaacattatc gtctgcagtt ttatatgttc gcatggactc 721 atgtcacttt actgataact gtcactcagt cacaccttgt catccaaaat ctgtttgaag 781 gcatgatatg gttccttgtt ccaatatcaa gtgttatctg caatgacata actgcttacc 841 tttttggatt tttttttggg agaactccat taattaagtt gtctcctaaa aagacttggg 901 aaggattcat tggtggtttc ttttccacag ttgtgtttgg attcattgct gcctatgtgt 961 tatccaaata ccagtacttt gtctgcccag tggaataccg aagtgatgta aactccttcg 1021 tgacagaatg tgagccctca gaacttttcc agcttcagac ttactcactt ccaccctttc 1081 taaaggcagt cttgagacag gaaagagtga gcttgtaccc tttccagatc cacagcattg 1141 cactgtcaac ctttgcatct ttaattggcc catttggagg cttctttgct agtggattca 1201 aaagagcctt caaaatcaag gattttgcaa ataccattcc tggacatggt gggataatgg 1261 acagatttga ttgtcagtat ttgatggcaa cttttgtaca tgtgtacatc acaagtttta 1321 taaggggccc aaatcccagc aaagtgctac agcagttgtt GGTGCTTCAA CCTGAACAGC 1381 AGTTAAATAT ATATAAAACC CTGAAGACTC ATCTCATTGA GAAAGGAATC CTACAACCCA 1441 CCTTGAAGGT ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1501 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1561 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT TATTCGGCCT CCTTTGTAGA 1621 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t