Construct: ORF TRCN0000477088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016342.1_s317c1
- Derived from:
- ccsbBroadEn_05690
- DNA Barcode:
- ATACACCGAAGCTAGGTACGCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM6-TRIM34 (445372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477088
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 445372 | TRIM6-TRIM34 | TRIM6-TRIM34 readthrough | NM_001003819.4 | 100% | 100% | |
| 2 | human | 53840 | TRIM34 | tripartite motif containing 34 | NM_001003827.1 | 57.9% | 57.9% | 0_1ins1062 |
| 3 | human | 53840 | TRIM34 | tripartite motif containing 34 | NM_021616.6 | 57.9% | 57.9% | 0_1ins1062 |
| 4 | human | 117854 | TRIM6 | tripartite motif containing 6 | NM_001003818.3 | 56.2% | 51.1% | (many diffs) |
| 5 | human | 117854 | TRIM6 | tripartite motif containing 6 | NM_058166.5 | 52.9% | 47.8% | (many diffs) |
| 6 | human | 117854 | TRIM6 | tripartite motif containing 6 | NM_001198644.2 | 32.3% | 27.2% | (many diffs) |
| 7 | human | 117854 | TRIM6 | tripartite motif containing 6 | NM_001198645.2 | 32.3% | 27.2% | (many diffs) |
| 8 | human | 53840 | TRIM34 | tripartite motif containing 34 | NM_130390.2 | 31.7% | 30.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2595
- ORF length:
- 2526
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgcgggtca gagaggattc tacaggcagg aaacatctta gaaatcaggg 121 ttgggcaggc aggagccagg agagtagcta caatgacttc accagtactg gtggacatac 181 gagaagaggt gacctgccct atctgcctgg agctcctaac agaacccctg agcatagact 241 gtggccacag cttctgccaa gcctgcatca caccaaatgg cagggaatca gtgattggtc 301 aagaagggga aagaagctgc cctgtgtgcc agaccagcta ccagccaggg aacctgcggc 361 ctaatcggca tctggccaac atagtgaggc ggctcagaga ggtagtgttg ggccctggga 421 agcagctgaa agcagttctt tgtgcagacc atggagaaaa actgcagctc ttctgtcagg 481 aggatgggaa ggtcatttgc tggctttgtg agcggtctca ggagcaccgt ggtcaccaca 541 cgttcctcgt ggaggaggtt gcccaggagt accaggagaa gtttcaggag tctctaaaga 601 agctgaagaa cgaggagcag gaagctgaga agctaacagc ttttatcaga gagaagaaaa 661 catcctggaa gaatcagatg gagcctgaga gatgcaggat ccagacagag tttaatcagc 721 tgcgaaatat cctagacaga gtggagcaac gggagctgaa aaagctggaa caggaagaga 781 agaaggggct acgaattata gaagaggctg agaatgatct ggtccaccag acccagtcgc 841 tgcgagagct catctcggat ctggagcgtc gatgtcaggg gtcaacaatg gagctgctgc 901 aggatgtgag tgatgtcaca gaaaggagtg agttctggac cctgaggaag ccagaagctc 961 tccctacaaa gctgagaagt atgttccgag ccccagatct gaaaaggatg ctgcgagtgt 1021 gtagagagct gacagatgtc caaagctact gggccatcca ggggtcttta accagaagag 1081 agaggagagc ctcaggagtt aggaccagaa gaagccaggg aagcagtgca atggcttcaa 1141 aaatcttgct taacgtacaa gaggaggtga cctgtcccat ctgcctggag ctgttgacag 1201 aacccttgag tctagactgt ggccacagcc tctgccgagc ctgcatcact gtgagcaaca 1261 aggaggcagt gaccagcatg ggaggaaaaa gcagctgtcc tgtgtgtggt atcagttact 1321 catttgaaca tctacaggct aatcagcatc tggccaacat agtggagaga ctcaaggagg 1381 tcaagttgag cccagacaat gggaagaaga gagatctctg tgatcatcat ggagagaaac 1441 tcctactctt ctgtaaggag gataggaaag tcatttgctg gctttgtgag cggtctcagg 1501 agcaccgtgg tcaccacaca gtcctcacgg aggaagtatt caaggaatgt caggagaaac 1561 tccaggcagt cctcaagagg ctgaagaagg aagaggagga agctgagaag ctggaagctg 1621 acatcagaga agagaaaact tcctggaagt atcaggtaca aactgagaga caaaggatac 1681 aaacagaatt tgatcagctt agaagcatcc taaataatga ggagcagaga gagctgcaaa 1741 gattggaaga agaagaaaag aagacgctgg ataagtttgc agaggctgag gatgagctag 1801 ttcagcagaa gcagttggtg agagagctca tctcagatgt ggagtgtcgg agtcagtggt 1861 caacaatgga gctgctgcag gacatgagtg gaatcatgaa atggagtgag atctggaggc 1921 tgaaaaagcc aaaaatggtt tccaagaaac tgaagactgt attccatgct ccagatctga 1981 gtaggatgct gcaaatgttt agagaactga cagctgtccg gtgctactgg gtggatgtca 2041 cactgaattc agtcaaccta aatttgaatc ttgtcctttc agaagatcag agacaagtga 2101 tatctgtgcc aatttggcct tttcagtgtt ataattatgg tgtcttggga tcccaatatt 2161 tctcctctgg gaaacattac tgggaagtgg acgtgtccaa gaaaactgcc tggatcctgg 2221 ggGTATACTG TAGAACATAT TCCCGCCATA TGAAGTATGT TGTTAGAAGA TGTGCAAATC 2281 GTCAAAATCT TTACACCAAA TACAGACCTC TATTTGGCTA CTGGGTTATA GGGTTACAGA 2341 ATAAATGTAA GTATGGTGTC TTTGAAGAGT CTTTGTCCTC TGATCCCGAG GTTTTGACTC 2401 TCTCCATGGC TGTGCCTCCC TGCCGTGTTG GGGTTTTCCT CGACTATGAA GCAGGCATTG 2461 TCTCATTTTT CAATGTCACA AGCCATGGCT CCCTCATTTA CAAGTTCTCT AAATGTTGCT 2521 TTTCTCAGCC TGTTTATCCA TATTTCAATC CTTGGAACTG TCCAGCTCCC ATGACTCTAT 2581 GCCCACCAAG CTCTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2641 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2701 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATACACCGAA GCTAGGTACG 2761 CAAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt