Construct: ORF TRCN0000477190
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006182.1_s317c1
- Derived from:
- ccsbBroadEn_03703
- DNA Barcode:
- GCGCCACGCGACGTTTCCCCGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGA6 (56109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477190
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56109 | PCDHGA6 | protocadherin gamma subfami... | NM_032086.1 | 100% | 100% | |
| 2 | human | 56109 | PCDHGA6 | protocadherin gamma subfami... | NM_018919.3 | 87.3% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2523
- ORF length:
- 2454
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcctccg cagaggcatc cgcagcgcag cgagcaggtc ctgctcctca 121 cgctcctggg gacgctgtgg ggggccgcgg cagcgcagat ccgctactct attcccgagg 181 agctggagaa aggctccttc gtaggcaaca tcgtcaagga tctgggactg gagccccagg 241 agttggcgga gcacggagtc cgcatcgtct ccagaggtag gatgcagctt ttctctctga 301 atccgcgaaa cggcagcttg gtcaccgcgg gtaggataga ccgcgaggag ctctgtgctc 361 agagcccgcg gtgtctggtg agttttaaca tccttgtcga ggataaactg aatctttatc 421 ccgtggaagt ggaaatagtg gacattaatg acaatacacc ccgattctta aaggaagaat 481 tggaagtgaa aattctcgaa aacgcagctc catcctctcg ttttccacta atggaggtct 541 atgaccctga tgtgggaatg aactcccttc agggatttaa gctcagtggt aatagtcact 601 tctcagtgga cgtgcaaagc gaagcccatg ggcccaagta cccggagctg gtgctggagg 661 gcacactgga ccgggaagga gaagccgttt accgcctggt ccttactgcc atggatggcg 721 gcgaccctgt ccgctcaagc gtcgcccaaa ttctggtaac agttctagat gtgaatgaca 781 acactccaat gtttactcag cctgtctacc gtgtaagtgt tcctgaaaac ctgccagtag 841 gcacaccagt gttggcagtg actgccaccg accaggatga aggagtccac ggggaagtaa 901 cttattcctt tgtgaagatt acagaaaaga tctcacaaat tttctgtttg aatgttttga 961 ctggagaaat ttcaacttct gcaaatctag actatgagga ctcgagtttt tatgagctgg 1021 gtgttgaagc ccgggatggg ccaggtcttc gagacagagc gaaagtctta ataactatct 1081 tggatgtcaa tgataatgta ccagaagtgg ttgttacatc tggaagcaga acaattgctg 1141 aaagtgcacc tccaggaaca gtaatcgccc tttttcaagt gttcgatcga gactctggcc 1201 tgaatggcct ggtaacctgt tccatcccga gaagtctccc atttgaattg gaaaaatcag 1261 ttggcaatta ttatcgatta gtgacaaatg cagctctaga ccgggaagag gtattcttgt 1321 acaacatcac tgtgacagcc acggacaaag gaacaccacc tctgtctaca gaaacaatca 1381 tctctctaaa tgtggcagac accaacgaca acccgcccac cttcccccat tcatcctact 1441 cagtctatgt ccttgaaaac aaccccaggg gtgcctccat cttctctgtg aatgcactgg 1501 accctgacgt ggaccagaac gcccaagtct cctactcact ggcagaagac accctccagg 1561 gggcgcccct gtcctcctac gtgtccatca actccgacac tgggattctg tacgccctgc 1621 gctccttcga ctatgagcag ttgagagacc tacagctgtg ggtgacagcc agcgacagcg 1681 gggacccgcc tcttagcagc aacgtgtcac tgagcctgtt tgtgctggac cagaatgaca 1741 atgcgcccga gatcctgtac cccgccctcc ccacagacgg ttccactggc gtggagctgg 1801 cgccccgctc cgcagagccc ggctacctgg tgaccaaggt ggtggcggtg gacagagact 1861 cgggccagaa cgcctggctg tcctaccgcc tgctcaaggc cagcgagcca ggacttttct 1921 cagtgggcct gcacacgggc gaggtgcgca cggcgcgcgc cctgctggac agagacgcgc 1981 tcaagcagag cctagtggtg gccgtccagg accacggcca gccccctctc tccgccactg 2041 tcacgctcac cgtggccgtg gccgacagga tccccgacat cctggccgac ctgggcagcc 2101 tcgagccctc cgccaaaccc aacgattcgg acctcactct gtacctggtg gtggcggtgg 2161 ccgcggtctc ctgcgtcttc ctggccttcg tcatcgtgct gctggcgctc agactgcagc 2221 gctggcacaa gtcacgcctg ctgcaggctt cgggaggtgg cttagcgagc atgcccggct 2281 cgcactttgt gggcgtggaa ggggttcggg ctttcctgca gacctattcc cacgaggtct 2341 cactcactgc agactcgcgt aagagtcatc tgattttccc ccagcccaac TATGCCGACA 2401 CGCTTATCAA CCAGGAGAGC TATGAGAAAA GCGAGCCTCT TCTGATAACT CAGGATTTAC 2461 TTGAAACGAA AGGAGAACCC AGGCAACTTC AGGTGAGTTT CTTTCCGCCT AAGCGGGAAG 2521 AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2581 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2641 GGCTTTATAT ATCTTGTGGA AAGGACGAGC GCCACGCGAC GTTTCCCCGC TCACGCGTTA 2701 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt