Construct: ORF TRCN0000477230
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013795.1_s317c1
- Derived from:
- ccsbBroadEn_06703
- DNA Barcode:
- ACAAGCGGTCCATTGACATACTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE4B (5142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477230
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_001037341.1 | 99.9% | 99.8% | 315G>N |
| 2 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_002600.4 | 99.9% | 99.8% | 315G>N |
| 3 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_001037340.2 | 91.8% | 88.9% | (many diffs) |
| 4 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_001297441.1 | 89.5% | 89.1% | (many diffs) |
| 5 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_001297440.1 | 87.3% | 87% | (many diffs) |
| 6 | human | 5142 | PDE4B | phosphodiesterase 4B | XM_017001445.1 | 79.3% | 77.7% | (many diffs) |
| 7 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_001037339.2 | 73% | 71.8% | (many diffs) |
| 8 | human | 5142 | PDE4B | phosphodiesterase 4B | XM_006710680.3 | 71.6% | 71.3% | (many diffs) |
| 9 | human | 5142 | PDE4B | phosphodiesterase 4B | XM_005270924.3 | 70.2% | 70.2% | 0_1ins657 |
| 10 | human | 5142 | PDE4B | phosphodiesterase 4B | XM_005270925.2 | 70.2% | 70.2% | 0_1ins657 |
| 11 | human | 5142 | PDE4B | phosphodiesterase 4B | NM_001297442.2 | 67.5% | 66.9% | (many diffs) |
| 12 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | XM_006502855.3 | 89.4% | 97% | (many diffs) |
| 13 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | NM_019840.2 | 82.3% | 86.5% | (many diffs) |
| 14 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | XM_006502856.2 | 81.3% | 81.9% | (many diffs) |
| 15 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | XM_006502857.3 | 79.7% | 85.4% | (many diffs) |
| 16 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | NM_001177980.1 | 67.3% | 71.2% | (many diffs) |
| 17 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | XM_017320029.1 | 64.6% | 70.5% | (many diffs) |
| 18 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | XM_017320030.1 | 63.2% | 69.1% | (many diffs) |
| 19 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | NM_001177981.1 | 61% | 65.8% | (many diffs) |
| 20 | mouse | 18578 | Pde4b | phosphodiesterase 4B, cAMP ... | NM_001177982.1 | 59.1% | 64.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2277
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaaaagc aggagtgtga tgacggtgat ggctgatgat aatgttaaag 121 attattttga atgtagcttg agtaaatcct acagttcttc cagtaacaca cttgggatcg 181 acctctggag agggagaagg tgttgctcag gaaacttaca gttaccacca ctgtctcaaa 241 gacagagtga aagggcaagg actcctgagg gagatggtat ttccaggccg accacactgc 301 ctttgacaac gcttccaagc attgctatta caactgtaag ccaggagtgc tttgatgtgg 361 aaaatggccc ttccccaggt cgnagtccac tggatcccca ggccagctct tccgctgggc 421 tggtacttca cgccaccttt cctgggcaca gccagcgcag agagtcattt ctctacagat 481 cagacagcga ctatgacttg tcaccaaagg cgatgtcgag aaactcttct cttccaagcg 541 agcaacacgg cgatgacttg attgtaactc cttttgccca ggtccttgcc agcttgcgaa 601 gtgtgagaaa caacttcact atactgacaa accttcatgg tacatctaac aagaggtccc 661 cagctgctag tcagcctcct gtctccagag tcaacccaca agaagaatct tatcaaaaat 721 tagcaatgga aacgctggag gaattagact ggtgtttaga ccagctagag accatacaga 781 cctaccggtc tgtcagtgag atggcttcta acaagttcaa aagaatgctg aaccgggagc 841 tgacacacct ctcagagatg agccgatcag ggaaccaggt gtctgaatac atttcaaata 901 ctttcttaga caagcagaat gatgtggaga tcccatctcc tacccagaaa gacagggaga 961 aaaagaaaaa gcagcagctc atgacccaga taagtggagt gaagaaatta atgcatagtt 1021 caagcctaaa caatacaagc atctcacgct ttggagtcaa cactgaaaat gaagatcacc 1081 tggccaagga gctggaagac ctgaacaaat ggggtcttaa catctttaat gtggctggat 1141 attctcacaa tagaccccta acatgcatca tgtatgctat attccaggaa agagacctcc 1201 taaagacatt cagaatctca tctgacacat ttataaccta catgatgact ttagaagacc 1261 attaccattc tgacgtggca tatcacaaca gcctgcacgc tgctgatgta gcccagtcga 1321 cccatgttct cctttctaca ccagcattag acgctgtctt cacagatttg gagatcctgg 1381 ctgccatttt tgcagctgcc atccatgacg ttgatcatcc tggagtctcc aatcagtttc 1441 tcatcaacac aaattcagaa cttgctttga tgtataatga tgaatctgtg ttggaaaatc 1501 atcaccttgc tgtgggtttc aaactgctgc aagaagaaca ctgtgacatc ttcatgaatc 1561 tcaccaagaa gcagcgtcag acactcagga agatggttat tgacatggtg ttagcaactg 1621 atatgtctaa acatatgagc ctgctggcag acctgaagac aatggtagaa acgaagaaag 1681 ttacaagttc aggcgttctt ctcctagaca actataccga tcgcattcag gtccttcgca 1741 acatggtaca ctgtgcagac ctgagcaacc ccaccaagtc cttggaattg tatcggcaat 1801 ggacagaccg catcatggag gaatttttcc agcagggaga caaagagcgg gagaggggaa 1861 tggaaattag cccaatgtgt gataaacaca cagcttctgt ggaaaaatcc caggttggtt 1921 tcatcgacta cattgtccat ccattgtggg agacatgggc agatttggta cagccTGATG 1981 CTCAGGACAT TCTCGATACC TTAGAAGATA ACAGGAACTG GTATCAGAGC ATGATACCTC 2041 AAAGTCCCTC ACCACCACTG GACGAGCAGA ACAGGGACTG CCAGGGTCTG ATGGAGAAGT 2101 TTCAGTTTGA ACTGACTCTC GATGAGGAAG ATTCTGAAGG ACCTGAGAAG GAGGGAGAGG 2161 GACACAGCTA TTTCAGCAGC ACAAAGACGC TTTGTGTGAT TGATCCAGAA AACAGAGATT 2221 CCCTGGGAGA GACTGACATA GACATTGCAA CAGAAGACAA GTCCCCCGTG GATACATTGC 2281 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GAACAAGCGG TCCATTGACA TACTCAACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt