Construct: ORF TRCN0000477264

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018140.1_s317c1
Derived from:
ccsbBroadEn_01188
DNA Barcode:
GAGACAGACCGACAGGTCTTATAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHEX (5251)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477264
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5251 PHEX phosphate regulating endope... NM_000444.6 100% 100%
2 human 5251 PHEX phosphate regulating endope... NM_001282754.2 92.4% 92.2% (many diffs)
3 human 5251 PHEX phosphate regulating endope... XM_024452390.1 87% 87% 0_1ins291
4 human 5251 PHEX phosphate regulating endope... XM_011545533.1 66.3% 66.3% 0_1ins756
5 human 5251 PHEX phosphate regulating endope... XM_017029579.1 66.3% 66.3% 0_1ins756
6 human 5251 PHEX phosphate regulating endope... XM_011545536.2 50.7% 50.7% 0_1ins1107
7 human 5251 PHEX phosphate regulating endope... XR_001755695.1 34.8% 1_679del;2082_2242del;3088_6444del
8 mouse 18675 Phex phosphate regulating endope... NM_011077.2 91.3% 95.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2316
ORF length:
2247
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaagcagaa acagggagca gcgtggagac tggaaagaag gccaacagag
121gcactcgaat tgccctggtc gtgtttgtcg gtggcaccct agttctgggc acgatcctct
181ttctagtgag tcaaggtctc ttaagtctcc aagctaaaca ggagtactgc ctgaagccag
241aatgcatcga agcggctgct gccatcttaa gtaaagtaaa tctgtctgtg gatccttgtg
301ataatttctt ccggttcgct tgtgatggct ggataagcaa taatccaatt cccgaagata
361tgccaagcta tggggtttat ccttggctga gacataatgt tgacctcaag ttgaaggaac
421ttttggagaa atcaatcagt agaaggcggg acaccgaagc catacagaaa gccaaaatcc
481tttattcatc ctgcatgaat gagaaagcga ttgaaaaagc agatgccaag ccactgctac
541acatcctacg gcattcacct ttccgctggc ccgtgcttga atctaatatt ggccctgaag
601gggtttggtc agagagaaag ttcagccttc tgcagacact tgcaacgttt cgtggtcaat
661acagcaattc tgtgttcatc cgtttgtatg tgtcccctga tgacaaagca tccaatgaac
721atatcttgaa gctggaccaa gcaacactct ccctggccgt gagggaagac taccttgata
781acagtacaga agccaagtct tatcgggatg ccctttacaa gttcatggtg gatactgccg
841tgcttttagg agctaacagt tccagagcag agcatgacat gaagtcagtg ctcagattgg
901aaattaagat agctgagata atgattccac atgaaaaccg aaccagcgag gccatgtaca
961acaaaatgaa catttctgaa ctgagtgcta tgattcccca gttcgactgg ctgggctaca
1021tcaagaaggt cattgacacc agactctacc cccatctgaa agacatcagc ccctccgaga
1081atgtggtggt ccgcgtcccg cagtacttta aagatttgtt taggatatta gggtctgaga
1141gaaagaagac cattgccaac tatttggtgt ggagaatggt ttattccaga attccaaacc
1201ttagcaggcg ctttcagtat agatggctgg aattctcaag ggtaatccag gggaccacaa
1261ctttgctgcc tcaatgggac aaatgtgtaa actttattga aagtgccctc ccttatgttg
1321ttggaaagat gtttgtagat gtgtacttcc aggaagataa gaaggaaatg atggaggaat
1381tggttgaggg cgttcgctgg gcctttattg acatgctaga gaaagaaaat gagtggatgg
1441atgcaggaac gaaaaggaaa gccaaagaaa aggcgagagc tgttttggca aaagttggct
1501atccagagtt tataatgaat gatactcatg ttaatgaaga cctcaaagct atcaagtttt
1561cagaagccga ctactttggc aacgtcctac aaactcgcaa gtatttagca cagtctgatt
1621tcttctggct aagaaaagcc gttccaaaaa cagagtggtt tacaaatccg acgactgtca
1681atgccttcta cagtgcatcc accaaccaga tccgatttcc agcaggagag ctccagaagc
1741ctttcttttg gggaacagaa tatcctcgat ctctgagtta tggtgctata ggagtaattg
1801tcggacatga atttacacat ggatttgata ataatggtag aaaatatgat aaaaatggaa
1861acctggatcc ttggtggtct actgaatcag aagaaaagtt taaggaaaaa acaaaatgca
1921tgattaacca gtatagcaac tattattgga agaaagctgg cttaaatgtc aaggggaaga
1981ggaccctggg agaaaatatt gctgataatg gaggcctgcg ggaagctttt agggcttaca
2041ggaaatggat aaatgacaga aggcagggac ttgaggagcc tcttctacca ggcaTCACAT
2101TCACCAACAA CCAGCTCTTC TTCCTGAGTT ATGCTCATGT GAGGTGCAAT TCCTACAGAC
2161CAGAAGCTGC CCGAGAACAA GTCCAAATTG GTGCTCACAG TCCCCCTCAG TTTAGGGTCA
2221ATGGTGCAAT TAGTAACTTT GAAGAATTCC AGAAAGCTTT TAACTGTCCA CCCAATTCCA
2281CGATGAACAG AGGCATGGAC TCCTGCCGAC TCTGGTTGCC AACTTTCTTG TACAAAGTGG
2341TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2401CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2461AGAGACAGAC CGACAGGTCT TATATACGCG TTAAGTCgac aatcaacctc tggattacaa
2521aatttgtgaa agatt

Download FASTA (ORF) (Full)