Construct: ORF TRCN0000477264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018140.1_s317c1
- Derived from:
- ccsbBroadEn_01188
- DNA Barcode:
- GAGACAGACCGACAGGTCTTATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHEX (5251)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5251 | PHEX | phosphate regulating endope... | NM_000444.6 | 100% | 100% | |
| 2 | human | 5251 | PHEX | phosphate regulating endope... | NM_001282754.2 | 92.4% | 92.2% | (many diffs) |
| 3 | human | 5251 | PHEX | phosphate regulating endope... | XM_024452390.1 | 87% | 87% | 0_1ins291 |
| 4 | human | 5251 | PHEX | phosphate regulating endope... | XM_011545533.1 | 66.3% | 66.3% | 0_1ins756 |
| 5 | human | 5251 | PHEX | phosphate regulating endope... | XM_017029579.1 | 66.3% | 66.3% | 0_1ins756 |
| 6 | human | 5251 | PHEX | phosphate regulating endope... | XM_011545536.2 | 50.7% | 50.7% | 0_1ins1107 |
| 7 | human | 5251 | PHEX | phosphate regulating endope... | XR_001755695.1 | 34.8% | 1_679del;2082_2242del;3088_6444del | |
| 8 | mouse | 18675 | Phex | phosphate regulating endope... | NM_011077.2 | 91.3% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2316
- ORF length:
- 2247
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagcagaa acagggagca gcgtggagac tggaaagaag gccaacagag 121 gcactcgaat tgccctggtc gtgtttgtcg gtggcaccct agttctgggc acgatcctct 181 ttctagtgag tcaaggtctc ttaagtctcc aagctaaaca ggagtactgc ctgaagccag 241 aatgcatcga agcggctgct gccatcttaa gtaaagtaaa tctgtctgtg gatccttgtg 301 ataatttctt ccggttcgct tgtgatggct ggataagcaa taatccaatt cccgaagata 361 tgccaagcta tggggtttat ccttggctga gacataatgt tgacctcaag ttgaaggaac 421 ttttggagaa atcaatcagt agaaggcggg acaccgaagc catacagaaa gccaaaatcc 481 tttattcatc ctgcatgaat gagaaagcga ttgaaaaagc agatgccaag ccactgctac 541 acatcctacg gcattcacct ttccgctggc ccgtgcttga atctaatatt ggccctgaag 601 gggtttggtc agagagaaag ttcagccttc tgcagacact tgcaacgttt cgtggtcaat 661 acagcaattc tgtgttcatc cgtttgtatg tgtcccctga tgacaaagca tccaatgaac 721 atatcttgaa gctggaccaa gcaacactct ccctggccgt gagggaagac taccttgata 781 acagtacaga agccaagtct tatcgggatg ccctttacaa gttcatggtg gatactgccg 841 tgcttttagg agctaacagt tccagagcag agcatgacat gaagtcagtg ctcagattgg 901 aaattaagat agctgagata atgattccac atgaaaaccg aaccagcgag gccatgtaca 961 acaaaatgaa catttctgaa ctgagtgcta tgattcccca gttcgactgg ctgggctaca 1021 tcaagaaggt cattgacacc agactctacc cccatctgaa agacatcagc ccctccgaga 1081 atgtggtggt ccgcgtcccg cagtacttta aagatttgtt taggatatta gggtctgaga 1141 gaaagaagac cattgccaac tatttggtgt ggagaatggt ttattccaga attccaaacc 1201 ttagcaggcg ctttcagtat agatggctgg aattctcaag ggtaatccag gggaccacaa 1261 ctttgctgcc tcaatgggac aaatgtgtaa actttattga aagtgccctc ccttatgttg 1321 ttggaaagat gtttgtagat gtgtacttcc aggaagataa gaaggaaatg atggaggaat 1381 tggttgaggg cgttcgctgg gcctttattg acatgctaga gaaagaaaat gagtggatgg 1441 atgcaggaac gaaaaggaaa gccaaagaaa aggcgagagc tgttttggca aaagttggct 1501 atccagagtt tataatgaat gatactcatg ttaatgaaga cctcaaagct atcaagtttt 1561 cagaagccga ctactttggc aacgtcctac aaactcgcaa gtatttagca cagtctgatt 1621 tcttctggct aagaaaagcc gttccaaaaa cagagtggtt tacaaatccg acgactgtca 1681 atgccttcta cagtgcatcc accaaccaga tccgatttcc agcaggagag ctccagaagc 1741 ctttcttttg gggaacagaa tatcctcgat ctctgagtta tggtgctata ggagtaattg 1801 tcggacatga atttacacat ggatttgata ataatggtag aaaatatgat aaaaatggaa 1861 acctggatcc ttggtggtct actgaatcag aagaaaagtt taaggaaaaa acaaaatgca 1921 tgattaacca gtatagcaac tattattgga agaaagctgg cttaaatgtc aaggggaaga 1981 ggaccctggg agaaaatatt gctgataatg gaggcctgcg ggaagctttt agggcttaca 2041 ggaaatggat aaatgacaga aggcagggac ttgaggagcc tcttctacca ggcaTCACAT 2101 TCACCAACAA CCAGCTCTTC TTCCTGAGTT ATGCTCATGT GAGGTGCAAT TCCTACAGAC 2161 CAGAAGCTGC CCGAGAACAA GTCCAAATTG GTGCTCACAG TCCCCCTCAG TTTAGGGTCA 2221 ATGGTGCAAT TAGTAACTTT GAAGAATTCC AGAAAGCTTT TAACTGTCCA CCCAATTCCA 2281 CGATGAACAG AGGCATGGAC TCCTGCCGAC TCTGGTTGCC AACTTTCTTG TACAAAGTGG 2341 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2401 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2461 AGAGACAGAC CGACAGGTCT TATATACGCG TTAAGTCgac aatcaacctc tggattacaa 2521 aatttgtgaa agatt