Construct: ORF TRCN0000477423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014827.1_s317c1
- Derived from:
- ccsbBroadEn_07253
- DNA Barcode:
- CCTAGGAGTTGTCCATAAAGAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4F2 (8529)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8529 | CYP4F2 | cytochrome P450 family 4 su... | NM_001082.5 | 99.8% | 99.8% | 74T>C;1542G>A |
| 2 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_001199208.2 | 94.5% | 92.6% | (many diffs) |
| 3 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_001199209.2 | 94.5% | 92.6% | (many diffs) |
| 4 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_001369696.1 | 94.5% | 92.6% | (many diffs) |
| 5 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_000896.3 | 89.4% | 87.5% | (many diffs) |
| 6 | human | 57834 | CYP4F11 | cytochrome P450 family 4 su... | NM_001128932.1 | 88.5% | 86% | (many diffs) |
| 7 | human | 57834 | CYP4F11 | cytochrome P450 family 4 su... | NM_021187.3 | 88.5% | 86% | (many diffs) |
| 8 | human | 11283 | CYP4F8 | cytochrome P450 family 4 su... | NM_007253.4 | 86% | 80.9% | (many diffs) |
| 9 | human | 11283 | CYP4F8 | cytochrome P450 family 4 su... | XM_024451340.1 | 86% | 80.9% | (many diffs) |
| 10 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | NM_023944.4 | 85.8% | 80.9% | (many diffs) |
| 11 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_017026815.1 | 69.6% | 66.9% | (many diffs) |
| 12 | human | 11283 | CYP4F8 | cytochrome P450 family 4 su... | XM_024451341.1 | 69.5% | 64.8% | (many diffs) |
| 13 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_005259911.5 | 68.5% | 68.6% | (many diffs) |
| 14 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_011528014.3 | 68.5% | 68.6% | (many diffs) |
| 15 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_017027172.1 | 68.4% | 54.8% | (many diffs) |
| 16 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | NR_117085.2 | 64.2% | (many diffs) | |
| 17 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_006722850.3 | 62.3% | 60.1% | (many diffs) |
| 18 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528202.3 | 62.3% | 60.1% | (many diffs) |
| 19 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528208.2 | 62.3% | 60.1% | (many diffs) |
| 20 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528203.2 | 58.2% | 56.2% | (many diffs) |
| 21 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528204.2 | 58.2% | 56.2% | (many diffs) |
| 22 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528205.2 | 58.2% | 56.2% | (many diffs) |
| 23 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_024451515.1 | 55.7% | 53.8% | (many diffs) |
| 24 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528207.2 | 55.7% | 54.1% | (many diffs) |
| 25 | human | 388514 | CYP4F24P | cytochrome P450 family 4 su... | NR_033864.1 | 38.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1629
- ORF length:
- 1560
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccagctg agcctgtcct ggctgggcct ctggccagtg gcagcatccc 121 cttggctgct cctcctgctg gccggggcct cctggctcct ggcccatgtc ctggcctgga 181 cctacgcctt ctatgacaac tgccgccgcc ttcggtgttt cccacaaccc ccaagacgga 241 actggttttg gggacaccag ggcatggtca accccacaga ggagggcatg agagttctga 301 ctcagctggt ggccacctac ccccagggct ttaaggtctg gatgggaccc atctcccccc 361 tcctcagttt gtgccacccc gacatcatcc ggtctgtcat caacgcctca gctgccattg 421 caccaaagga caagttcttc tacagcttcc tggagccctg gctgggggat gggctcctgc 481 tgagtgctgg tgacaagtgg agccgccacc gtcggatgct gacgcctgcc ttccatttca 541 acatcctgaa gccctatatg aagattttca atgagagtgt gaacatcatg cacgccaagt 601 ggcagctcct ggcctcagag ggtagtgcct gtttggatat gtttgagcac atcagcctca 661 tgaccttgga cagtctacag aaatgtgtct tcagctttga cagccattgt caggagaaac 721 ccagtgaata tattgccgcc atcttggagc tcagtgccct tgtatcaaaa agacaccatg 781 agatcctcct gcatattgac ttcctgtatt atctcacccc tgatgggcag cgtttccgca 841 gggcctgccg cctggtgcac gacttcacag atgccgtcat ccaggagcgg cgccgcactc 901 tccctagcca gggtgttgat gacttcctcc aagccaaggc caaatccaag actttggact 961 tcattgatgt actcctgctg agcaaggatg aagacgggaa gaagttatct gatgaggaca 1021 taagagcaga agctgacacc tttatgtttg agggccatga caccacggcc agtggtctct 1081 cctgggtcct gtaccacctt gcaaagcacc cagaatacca ggagcgctgc cggcaggagg 1141 tgcaagaact tctgaaggac cgtgagccta aagagattga atgggacgac ctggcccatt 1201 tgcccttcct gaccatgtgc atgaaggaga gcctgcggct gcaTCCCCCA GTCCCGGTCA 1261 TCTCCCGCCA TGTCACCCAG GACATTGTGC TCCCAGACGG CCGGGTCATC CCCAAAGGCA 1321 TTATCTGCCT CATCAGTGTT TTCGGAACCC ATCACAACCC AGCTGTGTGG CCGGACCCTG 1381 AGGTCTACGA CCCCTTTCGC TTTGACCCAG AGAACATCAA GGAGAGGTCA CCTCTGGCTT 1441 TTATTCCCTT CTCGGCAGGG CCCAGGAACT GCATCGGGCA GACGTTCGCG ATGGCGGAGA 1501 TGAAGGTGGT CCTGGCGCTC ACGCTGCTGC GCTTCCGCGT CCTGCCTGAC CACACCGAGC 1561 CCCGCAGGAA GCCGGAGCTG GTCCTGCGCG CAGAGGGCGG ACTTTGGCTA CGGGTGGAGC 1621 CCCTGAGCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1681 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1741 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTAGG AGTTGTCCAT AAAGAAGCAC 1801 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt