Construct: ORF TRCN0000477447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000919.1_s317c1
- Derived from:
- ccsbBroadEn_07220
- DNA Barcode:
- ATGAGCCGATCAGATTATCTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC45 (8318)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8318 | CDC45 | cell division cycle 45 | NM_003504.4 | 99.9% | 100% | 9G>A |
| 2 | human | 8318 | CDC45 | cell division cycle 45 | XM_024452277.1 | 99.7% | 99.8% | 9G>A;1560_1562delCAG |
| 3 | human | 8318 | CDC45 | cell division cycle 45 | NM_001369291.1 | 97.2% | 96.9% | (many diffs) |
| 4 | human | 8318 | CDC45 | cell division cycle 45 | XM_011530416.1 | 97.1% | 96.8% | (many diffs) |
| 5 | human | 8318 | CDC45 | cell division cycle 45 | NM_001178010.2 | 94.5% | 94.6% | 9G>A;541_636del |
| 6 | human | 8318 | CDC45 | cell division cycle 45 | NM_001178011.2 | 91.8% | 91.8% | 9G>A;201_202ins138 |
| 7 | human | 8318 | CDC45 | cell division cycle 45 | XM_011530418.3 | 91.8% | 91.8% | 9G>A;201_202ins138 |
| 8 | human | 8318 | CDC45 | cell division cycle 45 | XM_011530417.3 | 91.6% | 91.7% | 9G>A;201_202ins138;1422_1424delCAG |
| 9 | human | 8318 | CDC45 | cell division cycle 45 | NR_161281.1 | 78.4% | (many diffs) | |
| 10 | human | 8318 | CDC45 | cell division cycle 45 | XM_017028966.1 | 69.3% | 68.2% | (many diffs) |
| 11 | human | 8318 | CDC45 | cell division cycle 45 | XR_002958716.1 | 59.3% | (many diffs) | |
| 12 | human | 8318 | CDC45 | cell division cycle 45 | XM_017028967.1 | 58.3% | 58.3% | 0_1ins705;855_857delCAG |
| 13 | mouse | 12544 | Cdc45 | cell division cycle 45 | NM_009862.2 | 88.1% | 91.6% | (many diffs) |
| 14 | mouse | 12544 | Cdc45 | cell division cycle 45 | XM_006521744.2 | 88.1% | 91.6% | (many diffs) |
| 15 | mouse | 12544 | Cdc45 | cell division cycle 45 | XM_011245813.2 | 88.1% | 91.6% | (many diffs) |
| 16 | mouse | 12544 | Cdc45 | cell division cycle 45 | NM_001161623.1 | 81.3% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1764
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgtatccgat ttccgcaaag agttctacga ggtggtccag agccagaggg 121 tccttctctt cgtggcctcg gacgtggatg ctctgtgtgc gtgcaagatc cttcaggcct 181 tgttccagtg tgaccacgtg caatatacgc tggttccagt ttctgggtgg caagaacttg 241 aaactgcatt tcttgagcat aaagaacagt ttcattattt tattctcata aactgtggag 301 ctaatgtaga cctattggat attcttcaac ctgatgaaga cactatattc tttgtgtgtg 361 acacccatag gccagtcaat gtcgtcaatg tatacaacga tacccagatc aaattactca 421 ttaaacaaga tgatgacctt gaagttcccg cctatgaaga catcttcagg gatgaagagg 481 aggatgaaga gcattcagga aatgacagtg atgggtcaga gccttctgag aagcgcacac 541 ggttagaaga ggagatagtg gagcaaacca tgcggaggag gcagcggcga gagtgggagg 601 cccggagaag agacatcctc tttgactacg agcagtatga atatcatggg acatcgtcag 661 ccatggtgat gtttgagctg gcttggatgc tgtccaagga cctgaatgac atgctgtggt 721 gggccatcgt tggactaaca gaccagtggg tgcaagacaa gatcactcaa atgaaatacg 781 tgactgatgt tggtgtcctg cagcgccacg tttcccgcca caaccaccgg aacgaggatg 841 aggagaacac actctccgtg gactgcacac ggatctcctt tgagtatgac ctccgcctgg 901 tgctctacca gcactggtcc ctccatgaca gcctgtgcaa caccagctat accgcagcca 961 ggttcaagct gtggtctgtg catggacaga agcggctcca ggagttcctt gcagacatgg 1021 gtcttcccct gaagcaggtg aagcagaagt tccaggccat ggacatctcc ttgaaggaga 1081 atttgcggga aatgattgaa gagtctgcaa ataaatttgg gatgaaggac atgcgcgtgc 1141 agactttcag cattcatttt gggttcaagc acaagtttct ggccagcgac gtggtctttg 1201 ccaccatgtc tttgatggag agccccgaga aggatggctc agggacagat cacttcatcc 1261 aggctctgga cagcctctcc aggagtaacc tggacaagct gtaccatggc ctggaactcg 1321 ccaagaagca gctgcgagcc acccagcaga ccattgccag ctgcctttgc accaacctcg 1381 tcatctccca ggggcctttc ctgtactgct ctctcatgga gggcactcca gatgtcatgc 1441 tgttctctag gccggcatcc ctaagcctgc TCAGCAAACA CCTGCTCAAG TCCTTTGTGT 1501 GTTCGACAAA GAACCGGCGC TGCAAACTGC TGCCCCTGGT GATGGCTGCC CCCCTGAGCA 1561 TGGAGCATGG CACAGTGACC GTGGTGGGCA TCCCCCCAGA GACCGACAGC TCGGACAGGA 1621 AGAACTTTTT TGGGAGGGCG TTTGAGAAGG CAGCGGAAAG CACCAGCTCC CGGATGCTGC 1681 ACAACCATTT TGACCTCTCA GTAATTGAGC TGAAAGCTGA GGATCGGAGC AAGTTTCTGG 1741 ACGCACTTAT TTCCCTCCTG TCCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1801 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1861 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TGAGCCGATC 1921 AGATTATCTG CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1981 att