Construct: ORF TRCN0000477459

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016910.1_s317c1
Derived from:
ccsbBroadEn_06055
DNA Barcode:
AATTTCCATTAGTCTTCCTTATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CSNK1A1 (1452)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477459
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1452 CSNK1A1 casein kinase 1 alpha 1 NM_001892.6 99.9% 99.7% 691C>A
2 human 1452 CSNK1A1 casein kinase 1 alpha 1 NM_001271741.2 95.7% 95.8% 691C>A;972_974delTTT;975_976ins39
3 human 1452 CSNK1A1 casein kinase 1 alpha 1 NM_001025105.3 92.2% 92% 455_538del;775C>A
4 human 122011 CSNK1A1L casein kinase 1 alpha 1 like NM_145203.6 91.3% 90.2% (many diffs)
5 human 1452 CSNK1A1 casein kinase 1 alpha 1 NM_001271742.2 67.8% 67.6% 0_1ins267;188_271del;508C>A
6 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_006526388.2 94.8% 99.7% (many diffs)
7 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 NM_146087.2 90.7% 95.8% (many diffs)
8 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_006526384.2 87.5% 92% (many diffs)
9 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_006526385.2 87.3% 91.7% (many diffs)
10 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_011247048.2 85.3% 89.5% (many diffs)
11 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_006526383.3 85.2% 89.5% (many diffs)
12 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_006526386.3 84.9% 88.7% (many diffs)
13 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XM_006526387.2 83.7% 88.4% (many diffs)
14 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XR_386023.3 13.8% (many diffs)
15 mouse 93687 Csnk1a1 casein kinase 1, alpha 1 XR_386022.3 13.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1077
ORF length:
1011
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gagtagcagc ggctccaagg ctgaattcat tgtcggaggg aaatataaac
121tggtacggaa gatcgggtct ggctccttcg gggacatcta tttggcgatc aacatcacca
181acggcgagga agtggcagtg aagctagaat ctcagaaggc caggcatccc cagttgctgt
241acgagagcaa gctctataag attcttcaag gtggggttgg catcccccac atacggtggt
301atggtcagga aaaagactac aatgtactag tcatggatct tctgggacct agcctcgaag
361acctcttcaa tttctgttca agaaggttca caatgaaaac tgtacttatg ttagctgacc
421agatgatcag tagaattgaa tatgtgcata caaagaattt tatacacaga gacattaaac
481cagataactt cctaatgggt attgggcgtc actgtaataa gttattcctt attgattttg
541gtttggccaa aaagtacaga gacaacagga caaggcaaca cataccatac agagaagata
601aaaacctcac tggcactgcc cgatatgcta gcatcaatgc acatcttgGT ATTGAGCAGA
661GTCGCCGAGA TGACATGGAA TCATTAGGAT ATGTTTTGAT GTATTTTAAT AGAACCAGCC
721TGCCATGGCA AGGGCTAAAG GCTGCAACAA AGAAAAAAAA ATATGAAAAG ATTAGTGAAA
781AGAAGATGTC CACGCCTGTT GAAGTTTTAT GTAAGGGGTT TCCTGCAGAA TTTGCGATGT
841ACTTAAACTA TTGTCGTGGG CTACGCTTTG AGGAAGCCCC AGATTACATG TATCTGAGGC
901AGCTATTCCG CATTCTTTTC AGGACCCTGA ACCATCAATA TGACTACACA TTTGATTGGA
961CAATGTTAAA GCAGAAAGCA GCACAGCAGG CAGCCTCTTC CAGTGGGCAG GGTCAGCAGG
1021CCCAAACCCC CACAGGCAAG CAAACTGACA AAACCAAGAG TAACATGAAA GGTTTCTACC
1081CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1141TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1201ATATATCTTG TGGAAAGGAC GAAATTTCCA TTAGTCTTCC TTATAAACGC GTTAAGTCga
1261caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)