Construct: ORF TRCN0000477523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009420.1_s317c1
- Derived from:
- ccsbBroadEn_07853
- DNA Barcode:
- TTAAAATCGCCAGCACTACGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMPCA (23203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | NM_015160.3 | 99.9% | 100% | 702C>T |
| 2 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | XM_005266059.4 | 88% | 83.7% | (many diffs) |
| 3 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | NM_001282946.1 | 80.8% | 72.6% | 0_1ins298;137_138insAG;402C>T |
| 4 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | NM_001282944.1 | 74.9% | 64.2% | 0_1ins298;137_138ins95;309C>T |
| 5 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | XM_017014543.2 | 65.2% | 65.3% | 0_1ins546;156C>T |
| 6 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | XM_011518417.3 | 56.2% | 52% | (many diffs) |
| 7 | mouse | 66865 | Pmpca | peptidase (mitochondrial pr... | NM_173180.3 | 85.1% | 91.2% | (many diffs) |
| 8 | mouse | 66865 | Pmpca | peptidase (mitochondrial pr... | XM_006498237.3 | 60.5% | 65.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1644
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggctgtg gtgctggcgg cgacgcggtt gctgcggggc tcgggttctt 121 ggggctgttc gcggctgagg tttggacctc ctgcgtacag acggtttagt agtggtggtg 181 cctatcccaa catccccctc tcttctccct tacctggagt acccaagcct gtttttgcta 241 cagttgatgg acaggaaaag tttgaaacca aagtaaccac attggataat gggcttcgcg 301 tggcatctca gaataagttt ggacagtttt gtacagtagg aattcttatc aattcaggat 361 cgagatatga agcgaaatac cttagtggaa ttgctcactt tttggaaaaa ttggcatttt 421 cgtctactgc tcgatttgac agcaaagatg aaattctgct tacgttggaa aagcatgggg 481 gtatctgtga ctgccagaca tcaagagaca ccaccatgta tgctgtgtct gctgatagca 541 aaggcttgga cacggtggtt gccttactgg ctgatgtggt tctgcagccc cggctaacag 601 atgaagaagt cgagatgacg cggatggcgg tccagtttga gctggaggac ctgaacctgc 661 ggcctgaccc agagccactt ctcaccgaga tgattcatga agcggcttac agggagaaca 721 cagttggcct ccaccgtttc tgccccacag aaaacgtagc aaagatcaat cgagaggtgc 781 tgcattccta cctgaggaac tactacactc ccgaccgcat ggtgctggcc ggcgtgggcg 841 tggagcacga gcatctggtg gactgtgccc ggaagtacct cctgggggtc cagccggcct 901 gggggagcgc agaggccgtg gatattgaca gatctgtggc ccagtacact ggggggattg 961 ccaagctaga aagagacatg tccaatgtca gcctgggccc gacccccatc cccgagctca 1021 cgcacatcat ggttggactg gagagctgct ccttcctgga ggaggacttc atcccctttg 1081 cagtgttgaa catgatgatg ggcggaggtg gctccttctc ggctggtggg cccggcaagg 1141 gcatgttctc caggctctac ctcaacgtgc tcaacaggca ccactggatg tataacgcga 1201 cctcctacca ccacagctac gaggacactg gcctcctttg catccatgcc agcgccgacc 1261 caagacaggt tcgagaaatg gtagaaatca TCACAAAGGA GTTTATTTTA ATGGGCGGAA 1321 CCGTGGACAC GGTGGAGCTG GAACGAGCCA AGACGCAGCT GACATCAATG CTCATGATGA 1381 ACCTGGAATC CAGGCCTGTG ATCTTCGAGG ATGTGGGGAG GCAGGTGCTG GCCACTCGCT 1441 CCAGAAAGCT GCCGCACGAG CTGTGCACGC TCATCCGCAA CGTGAAGCCG GAAGATGTGA 1501 AGAGAGTCGC TTCTAAGATG CTCCGAGGGA AGCCGGCAGT GGCCGCCCTG GGTGACCTGA 1561 CTGACCTGCC CACGTATGAG CACATCCAGA CCGCCCTGTC GAGTAAGGAC GGGCGCCTGC 1621 CCAGGACGTA CCGGCTCTTC CGGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TAAAATCGCC 1801 AGCACTACGC CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att