Construct: ORF TRCN0000477550
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011959.1_s317c1
- Derived from:
- ccsbBroadEn_02880
- DNA Barcode:
- GGCTCTTTACCACCTTGGTCTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAMR1 (25891)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477550
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25891 | PAMR1 | peptidase domain containing... | NM_015430.4 | 100% | 100% | |
| 2 | human | 25891 | PAMR1 | peptidase domain containing... | NM_001001991.3 | 97.6% | 97.6% | 820_821ins51 |
| 3 | human | 25891 | PAMR1 | peptidase domain containing... | NM_001282675.1 | 92.2% | 92.2% | 0_1ins120;700_701ins51 |
| 4 | human | 25891 | PAMR1 | peptidase domain containing... | NM_001282676.1 | 82.6% | 82.4% | 379_380ins333;487_488ins51 |
| 5 | mouse | 210622 | Pamr1 | peptidase domain containing... | NM_173749.4 | 84.2% | 88.1% | (many diffs) |
| 6 | mouse | 210622 | Pamr1 | peptidase domain containing... | XR_001781792.1 | 40.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2280
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctgggt tgctggacgc agttggggct cacttttctt cagctccttc 121 tcatctcgtc cttgccaaga gagtacacag tcattaatga agcctgccct ggagcagagt 181 ggaatatcat gtgtcgggag tgctgtgaat atgatcagat tgagtgcgtc tgccccggaa 241 agagggaagt cgtgggttat accatccctt gctgcaggaa tgaggagaat gagtgtgact 301 cctgcctgat ccacccaggt tgtaccatct ttgaaaactg caagagctgc cgaaatggct 361 catggggggg taccttggat gacttctatg tgaaggggtt ctactgtgca gagtgccgag 421 caggctggta cggaggagac tgcatgcgat gtggccaggt tctgcgagcc ccaaagggtc 481 agattttgtt ggaaagctat cccctaaatg ctcactgtga atggaccatt catgctaaac 541 ctgggtttgt catccaacta agatttgtca tgttgagcct ggagtttgac tacatgtgcc 601 agtatgacta tgttgaggtt cgtgatggag acaaccgcga tggccagatc atcaagcgtg 661 tctgtggcaa cgagcggcca gctcctatcc agagcatagg atcctcactc cacgtcctct 721 tccactccga tggctccaag aattttgacg gtttccatgc catttatgag gagatcacag 781 catgctcctc atccccttgt ttccatgacg gcacgtgcgt ccttgacaag gctggatctt 841 acaagtgtgc ctgcttggca ggctatactg ggcagcgctg tgaaaatctt ctggaggctg 901 ggaagtccaa gatcaaggcg tcagaagatt cattgtctgt ccttgaagaa agaaactgct 961 cagaccctgg gggcccagtc aatgggtacc agaaaataac agggggccct gggcttatca 1021 acggacgcca tgctaaaatt ggcaccgtgg tgtctttctt ttgtaacaac tcctatgttc 1081 ttagtggcaa tgagaaaaga acttgccagc agaatggaga gtggtcaggg aaacagccca 1141 tctgcataaa agcctgccga gaaccaaaga tttcagacct ggtgagaagg agagttcttc 1201 cgatgcaggt tcagtcaagg gagacaccat tacaccagct atactcagcg gccttcagca 1261 agcagaaact gcagagtgcc cctaccaaga agccagccct tccctttgga gatctgccca 1321 tgggatacca acatctgcat acccagctcc agtatgagtg catctcaccc ttctaccgcc 1381 gcctgggcag cagcaggagg acatgtctga ggactgggaa gtggagtggg cgggcaccat 1441 cctgcatccc tatctgcggg aaaattgaga acatcactgc tccaaagacc caagggttgc 1501 gctggccgtg gcaggcagcc atctacagga ggaccagcgg ggtgcatgac ggcagcctac 1561 acaagggagc gtggttccta gtctgcagcg gtgccctggt gaatgagcgc actgtggtgg 1621 tggctgccca ctgtgttact gacctgggga aggtcaccat gatcaagaca gcagacctga 1681 aagttgtttt ggggaaattc taccgggatg atgaccggga tgagaagacc atccagagcc 1741 tacagatttc tgctatcatt ctgcatccca actatgaccc catcctgctt gatgctgaca 1801 tcgccatcct gaagctccta gacaaggccc gtatcagcac ccgagtccag cccatctgcc 1861 tcgctgccag tcgggatctc agcacttcct tccaggagTC CCACATCACT GTGGCTGGCT 1921 GGAATGTCCT GGCAGACGTG AGGAGCCCTG GCTTCAAGAA CGACACACTG CGCTCTGGGG 1981 TGGTCAGTGT GGTGGACTCG CTGCTGTGTG AGGAGCAGCA TGAGGACCAT GGCATCCCAG 2041 TGAGTGTCAC TGATAACATG TTCTGTGCCA GCTGGGAACC CACTGCCCCT TCTGATATCT 2101 GCACTGCAGA GACAGGAGGC ATCGCGGCTG TGTCCTTCCC GGGACGAGCA TCTCCTGAGC 2161 CACGCTGGCA TCTGATGGGA CTGGTCAGCT GGAGCTATGA TAAAACATGC AGCCACAGGC 2221 TCTCCACTGC CTTCACCAAG GTGCTGCCTT TTAAAGACTG GATTGAAAGA AATATGAAAT 2281 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2341 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2401 TTTATATATC TTGTGGAAAG GACGAGGCTC TTTACCACCT TGGTCTCTGA CGCGTTAAGT 2461 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt