Construct: ORF TRCN0000477634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007125.1_s317c1
- Derived from:
- ccsbBroadEn_03094
- DNA Barcode:
- ATTCTTTCTGAAAAATAACACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZDHHC1 (29800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | NM_013304.3 | 100% | 100% | |
| 2 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | XM_024450245.1 | 100% | 100% | |
| 3 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | XM_006721185.2 | 93.9% | 91% | (many diffs) |
| 4 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | NM_001323627.2 | 89.5% | 69% | (many diffs) |
| 5 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | XM_024450246.1 | 89.4% | 77.3% | 1042_1043ins104;1352_1407del |
| 6 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | XM_024450247.1 | 89% | 89% | 1010_1011ins159 |
| 7 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | XM_011523060.1 | 82.4% | 82.4% | 0_1ins255 |
| 8 | human | 29800 | ZDHHC1 | zinc finger DHHC-type conta... | XM_024450248.1 | 73.4% | 71.9% | 1042_1043ins124;1068_1069ins263 |
| 9 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | NM_175160.3 | 76% | 68.3% | (many diffs) |
| 10 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_006531357.3 | 76% | 68.3% | (many diffs) |
| 11 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_011248500.2 | 76% | 68.3% | (many diffs) |
| 12 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_011248501.2 | 76% | 68.3% | (many diffs) |
| 13 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_017312960.1 | 75.6% | 67.5% | (many diffs) |
| 14 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_011248502.1 | 75.5% | 73.3% | (many diffs) |
| 15 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_006531358.3 | 75.2% | 67.7% | (many diffs) |
| 16 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_017312961.1 | 74.8% | 66.9% | (many diffs) |
| 17 | mouse | 70796 | Zdhhc1 | zinc finger, DHHC domain co... | XM_006531360.3 | 61.7% | 48.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta caagatgaac atctgcaaca agccctccaa caagacggcc cctgagaaga 121 gtgtgtggac ggcaccggca cagcccagcg gaccctcccc tgagctgcag ggccagcgat 181 cccgccggaa tgggtggagc tggccccctc acccgctcca gattgtggcc tggctgctgt 241 acctcttctt tgctgtgatc ggctttggga tccttgttcc cctcctgcct caccactggg 301 tgcccgctgg ctacgcttgc atgggcgcca tctttgctgg ccaccttgtg gtgcacctga 361 ccgccgtctc catcgatcca gcagatgcca acgtgcggga caagagctat gcggggcccc 421 tgcccatctt caaccgaagc cagcacgcac atgtcattga agacctgcac tgcaacttgt 481 gcaacgtgga tgtgagcgct cgctccaagc actgcagcgc ctgcaacaag tgcgtgtgcg 541 gtttcgacca ccactgcaag tggctcaaca actgtgtggg cgagcggaac taccggctct 601 ttctacacag tgttgcatcc gctttactgg gcgtcctgct cctggtgctg gtggccacat 661 atgtcttcgt ggagttcttt gtcaacccca tgcgtctgcg caccaaccga cactttgaag 721 tcctgaagaa tcacacggat gtgtggttcg tgttcctgcc tgccgccccc gtggagaccc 781 aggcccctgc catcctggcc ctggccgccc tgctcatcct tctgggcctc ctgtccacag 841 ccctcctggg gcacctgctc tgcttccaca tttatctcat gtggcacaag ctcaccacct 901 atgagtacat cgtgcagcac cgcccaccac aggaggccaa gggggttcac agggagctcg 961 agtcatgtcc tcccaagatg cggcccattc aggagatgga gttctacatg cggaccttca 1021 gacatatgcg cccagagccc cctggccagg ccgggccagc agcagtgaat gccaaacact 1081 ctcgccctgc ctccccggat ccgaccccag gtaggaggga ctgtgctggg cctccggtcc 1141 aggtggagtg ggatagaaag aagcctctac cctggcgctc gcctctgctt cttttggcga 1201 tgtggggccc tcaggctccc ccgtgtctct gcagaaaaag aggaagaggc gcgtgtataa 1261 agtgcgaacg tctgagacct cggatccggc gtcggggccT AGGGCCCCCA GCCGCCGCTC 1321 CAGCTCGTCG ACGGATTCCG CGGACGCCAG CCCTGTGCAC GCCGCTGGCC CTGCCGGCGC 1381 CTACCACTCG GCGTCGGCAG AGTCCGTGGA CGAGATTCCA GTGGCGCAGA CGCGCCTGGG 1441 CAGCGCCGCT CTGGCCGCCC CGCGGGGCCG GGGCCGACAG CCCACGCTGG CGCGGCAGGC 1501 GCGTGCGCCC GCCGTTTTCG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATTC TTTCTGAAAA 1681 ATAACACTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt