Construct: ORF TRCN0000477717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017142.2_s317c1
- Derived from:
- ccsbBroadEn_13633
- DNA Barcode:
- ATTCCACATTACTGCATATGACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PI4KAP2 (375133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375133 | PI4KAP2 | phosphatidylinositol 4-kina... | NR_003700.1 | 75.9% | (many diffs) | |
| 2 | human | 728233 | PI4KAP1 | phosphatidylinositol 4-kina... | NR_003563.1 | 66.8% | (many diffs) | |
| 3 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_017028830.1 | 38.3% | 36.6% | (many diffs) |
| 4 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_005261635.1 | 30.4% | 29.1% | (many diffs) |
| 5 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362862.1 | 27.2% | 26% | (many diffs) |
| 6 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362863.1 | 27.1% | 25.9% | (many diffs) |
| 7 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_058004.4 | 26.8% | 25.6% | (many diffs) |
| 8 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XR_937868.1 | 23.2% | (many diffs) | |
| 9 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522037.2 | 43.3% | 44.9% | (many diffs) |
| 10 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | NM_001001983.2 | 24.8% | 25.8% | (many diffs) |
| 11 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522034.1 | 24.1% | 25.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1797
- ORF length:
- 1731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cccccaggaa ggccacgcgc tcaggaggcc ccccccccgc tggctgctgc 121 tcacatggtg tctgggccct tggcactccg ttcatgcggg agatgacagg ggctggcaca 181 tgacagtgga gcagaaattt ggcctgtttt ctgctgagat aaaggaagca gaccccctgg 241 ctgcctcgga agcaagtcaa cccaaaccct gtccccccga agtgaccccc cactacatct 301 ggatcgactt cctggtgcag cggtttgaga tcgccaagta ctgcagctct gaccaagtgg 361 agatcttctc cagcctgctg cagcgctcca tgtccctgaa catcggcagg gccaagggga 421 gcatgaaccg gcacgtggcg gccatcgggc cccgcttcaa gctgctgacc ctggggctgt 481 ccctcctgca tgccgatgtg gttccaaatg caaccatccg caatgtgctt cgcgagaaga 541 tctactccac tgcctttgac tacttcagct gtcccccaaa gtttcctact caaggagaga 601 agcggctgcg tgaagacata agcatcatga ttaaattttg gaccgccatg ttctcagata 661 agaagtacct gaccgccagc cagcttgttc ccccagctga catcggcgac ctcctggagc 721 agttagtaga ggagaacaca ggctccttgt cgggcccagc gaaggacttt taccagcggg 781 agtttgattt ctttaacaag atcaccaacg tgtcggctat catcaagccc taccctaaag 841 gcgacgagag aaagaaggct tgtctgtcgg ccctgtctga agtgacggtg cagccaggct 901 gctccctgcc cagcaacccc gaagccattg tgctggacgt cgactacaag tctgggaccc 961 cgatgcagag tgctgcaaaa gccccatatc tggccaagtt caaggtgaag cgatgtggag 1021 ttagtgaact tgaaaaagaa ggtctgcggt gccgctcaga ctctgaggat gagtgcagca 1081 cgcaggaggc cgacggccag aagatctcct ggcaggcagc catcttcaaa ctgggagacg 1141 actgccggca ggacatgctg gccctgcaga tcatcgacct cttcaagaac atcttccagc 1201 tggtcggcct ggacctcttt gtttttccct accgcgtggt ggccactgcc cctgggtgcg 1261 gggtgatcga gtgcatcccc gactgcacct cccgggacca gctgggccgc cagacagact 1321 tcggcatgta cgactacttc acacgccagt acggggatga gtccactctg gccttccagc 1381 aggcccgcta caacttcatc cgaagcatgg ccgcctacag cctcctgctg ttcctgctgc 1441 agatcaagga cagacacaac ggcaacatta tgctggacaa gaagggccat atcatccaca 1501 ttgactttgg cttcatgttt GAAAGCTCGC CGGGCGGCAA TCTCGGCTGG GAACCCGACA 1561 TCAAGCTGAC GGATGAGATG GTGATGATCA TGGGGGGCAA GATGGAGGCC ACACCCTTCA 1621 AGTGGTTCAT GGAGATGTGT GTCCGAGGCT ACCTGGCTGT GCGGCACAGG TTTAGCCCCA 1681 ACATGACTGA GCGCGAGGCT GCAAATTTCA TCATGAAGGT CATCCAGAGC TGCTTCCTCA 1741 GCAACAGGAG CCGGACCTAC AACATGATCC AGTACTATCA GAATGACATC CCCTACTGCC 1801 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1861 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1921 ATATATCTTG TGGAAAGGAC GAATTCCACA TTACTGCATA TGACGGACGC GTTAAGTCga 1981 caatcaacct ctggattaca aaatttgtga aagatt