Construct: ORF TRCN0000477743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003093.1_s317c1
- Derived from:
- ccsbBroadEn_10011
- DNA Barcode:
- TGCCCTAATGTTTGGACCTAGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF260 (339324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477743
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 339324 | ZNF260 | zinc finger protein 260 | NM_001012756.3 | 99.9% | 100% | 1050T>C |
| 2 | human | 339324 | ZNF260 | zinc finger protein 260 | NM_001166036.2 | 99.9% | 100% | 1050T>C |
| 3 | human | 339324 | ZNF260 | zinc finger protein 260 | NM_001166037.2 | 99.9% | 100% | 1050T>C |
| 4 | human | 339324 | ZNF260 | zinc finger protein 260 | NM_001166038.2 | 99.9% | 100% | 1050T>C |
| 5 | human | 339324 | ZNF260 | zinc finger protein 260 | XM_011526897.2 | 99.9% | 100% | 1050T>C |
| 6 | human | 339324 | ZNF260 | zinc finger protein 260 | XM_017026741.1 | 99.9% | 100% | 1050T>C |
| 7 | mouse | 26466 | Zfp260 | zinc finger protein 260 | NM_011981.4 | 83.4% | 85.6% | (many diffs) |
| 8 | mouse | 26466 | Zfp260 | zinc finger protein 260 | XM_011250583.1 | 83.4% | 85.6% | (many diffs) |
| 9 | mouse | 26466 | Zfp260 | zinc finger protein 260 | XM_011250584.2 | 83.4% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1305
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gataggcatg ttggaaagtc ttcagcatga atcagatctc cttcagcatg 121 atcaaattca tactggagag aaaccttatg aatgtaatga atgtagaaaa acttttagcc 181 tgaagcaaaa ccttgtagag cataagaaaa tgcatactgg agagaaatct catgaatgca 241 ctgaatgtgg taaagtgtgc tctcgagtct catctcttac tctacacctt agaagtcaca 301 caggaaagaa ggcatataaa tgtaataaat gtggaaaagc cttcagccag aaggaaaact 361 tcctttctca tcagaaacat cacactggag agaaacctta tgaatgtgaa aaagtttcta 421 ttcagatgcc aaccatcatc agacaccaga aaaatcatac aggaaccaaa ccctatgcat 481 gtaaggaatg tggcaaagcc tttaacggca aagcatatct cactgagcat gagaaaattc 541 atactggaga aaaaccattt gaatgtaatc agtgtggaag agccttcagc cagaagcaat 601 acctcattaa acatcagaac atccatactg gaaagaagcc ctttaaatgt agtgagtgtg 661 gaaaagcttt tagccagaag gaaaacctca ttatacatca gagaatccat actggagaga 721 aaccttatga atgtaaaggg tgtgggaaag ctttcattca gaagtcaagc ctcattagac 781 accagagaag tcacacagga gagaaacctt atacgtgtaa ggaatgtggg aaagccttca 841 gtggcaagtc aaatctcaca gagcatgaga aaattcatat tggagagaaa cccTACAAAT 901 GTAATGAATG TGGAACAATC TTCAGGCAGA AGCAATACCT CATTAAACAT CACAATATTC 961 ATACAGGAGA GAAACCCTAT GAATGTAATA AATGTGGAAA AGCCTTCTCT CGAATCACAT 1021 CACTTATTGT ACATGTGAGA ATTCATACAG GTGATAAGCC TTATGAGTGT AAGGTCTGTG 1081 GGAAAGCCTT CTGTCAAAGC TCATCTCTTA CTGTGCACAT GAGAAGCCAT ACAGGTGAGA 1141 AACCCTATGG TTGTAATGAA TGTGGGAAAG CCTTTTCTCA GTTCTCAACC CTTGCTCTGC 1201 ACATGAGAAT CCATACTGGT GAAAAACCTT ATCAGTGTAG TGAATGTGGG AAAGCTTTCA 1261 GCCAAAAGTC ACATCACATT AGACACCAGA GAATTCATAC TCATTTGCCA ACTTTCTTGT 1321 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1381 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1441 GAAAGGACGA TGCCCTAATG TTTGGACCTA GTTTACGCGT TAAGTCgaca atcaacctct 1501 ggattacaaa atttgtgaaa gatt