Construct: ORF TRCN0000477805

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005959.1_s317c1
Derived from:
ccsbBroadEn_04992
DNA Barcode:
TACATTACAGCGACGATCTTTAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TOM1L2 (146691)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477805
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001033551.3 100% 100%
2 human 146691 TOM1L2 target of myb1 like 2 membr... XM_011523662.1 94% 94% 1128_1214del
3 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001288787.2 91.6% 88.9% (many diffs)
4 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001082968.2 90.1% 90.1% 217_366del
5 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001350331.2 87.4% 84.6% (many diffs)
6 human 146691 TOM1L2 target of myb1 like 2 membr... XM_005256462.1 86.4% 83.7% (many diffs)
7 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001350333.2 86.1% 86.1% 217_366del;1277_1278ins60
8 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001350332.2 85.2% 85.2% 217_366del;1278_1364del
9 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001288788.2 80% 76.2% (many diffs)
10 human 146691 TOM1L2 target of myb1 like 2 membr... XM_005256466.1 79.3% 75.8% (many diffs)
11 human 146691 TOM1L2 target of myb1 like 2 membr... XM_024450589.1 75.7% 75.7% 217_366del;500_501ins159;1118_1119ins60
12 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001288786.2 75.3% 75.3% 217_366del;500_501ins159;1119_1205del
13 human 146691 TOM1L2 target of myb1 like 2 membr... NM_001288789.2 73.8% 73.8% 216_217ins294;834_920del
14 human 146691 TOM1L2 target of myb1 like 2 membr... XR_933976.2 20.4% (many diffs)
15 mouse 216810 Tom1l2 target of myb1-like 2 (chic... NM_153080.2 79% 85.2% (many diffs)
16 mouse 216810 Tom1l2 target of myb1-like 2 (chic... XM_011248906.2 76.1% 81.8% (many diffs)
17 mouse 216810 Tom1l2 target of myb1-like 2 (chic... XM_006532861.3 76% 81.8% (many diffs)
18 mouse 216810 Tom1l2 target of myb1-like 2 (chic... NM_001039092.3 75.3% 81.2% (many diffs)
19 mouse 216810 Tom1l2 target of myb1-like 2 (chic... XM_011248907.2 70.5% 75.5% (many diffs)
20 mouse 216810 Tom1l2 target of myb1-like 2 (chic... XM_011248908.2 68.1% 71.9% (many diffs)
21 mouse 216810 Tom1l2 target of myb1-like 2 (chic... XM_006532867.3 67.2% 68% (many diffs)
22 mouse 216810 Tom1l2 target of myb1-like 2 (chic... NM_001039093.1 66.4% 70.3% (many diffs)
23 mouse 216810 Tom1l2 target of myb1-like 2 (chic... XM_017314451.1 61.6% 61.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1437
ORF length:
1371
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gttcctcctg gggaacccgt tcagcacacc agtggggcag tgcctcgaaa
121aggcaacaga tggctccctg caaagtgagg attggacgtt gaatatggag atctgtgaca
181tcatcaatga gacggaggaa gggccaaagg atgccattcg agccctgaag aagcggctca
241acgggaaccg gaactacaga gaggtgatgc tggcattaac agcatgggct gatgcctttc
301gaagcagtcc tgatctcacc ggcgttgtgc acatatatga ggagctgaag aggaaagggg
361ttgaatttcc catggcagac ttggacgctc tgtctcccat acacacacca cagcggagtg
421tccctgaagt ggatccagct gcgaccatgc ccaggtccca atcacagcag aggacaagtg
481ctggttccta ttcctcgccg cctcctgctc cctactccgc accgcaggcc ccagctctga
541gtgtgactgg ccccatcaca gccaattcag aacagattgc caggctgcgg agtgaactgg
601acgtcgttcg aggaaacaca aaagtcatgt ctgagatgtt aacagaaatg gtccctggac
661aggaggattc atctgatctg gagttgctgc aggagctcaa caggacctgt cgggccatgc
721agcagcgcat cgtggagctc atctcccgcg tgtccaatga ggaggtcacc gaggagctgc
781tgcatgtgaa cgatgacctc aacaacgtct tccttcgata cgagaggttc gaacgataca
841ggtctggccg atccgttcaa aatgccagta atggagtact gaatgaagta accgaagaca
901acttaataga cctggggcca gggtctccag ccgtggtgag cccaatggtg gggaacacag
961cgcccccatc ttccctctcc tcccagcttg caggcttaga cttggggaca gagagcgtca
1021gtggcaccct cagttcactc cagcaatgta atccccgtga cggctttgac atgtttgccc
1081agacgagagg aaactccttg gctgagcagc gcaagacggt aacctatgag gatccTCAGG
1141CTGTCGGAGG ACTTGCTTCT GCACTAGACA ATCGAAAACA GAGTTCAGAA GGGATCCCCG
1201TTGCGCAGCC ATCTGTCATG GACGACATTG AGGTGTGGCT CAGGACCGAC CTGAAGGGTG
1261ATGATCTGGA GGAGGGTGTC ACAAGTGAAG AGTTTGATAA ATTCCTTGAA GAAAGAGCCA
1321AAGCTGCTGA AATGGTTCCC GACCTCCCCT CGCCCCCCAT GGAGGCTCCT GCCCCAGCCT
1381CAAACCCTTC TGGCCGGAAG AAGCCAGAGC GGTCAGAGGA TGCCCTCTTC GCCCTGTGCC
1441CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1501TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1561ATATATCTTG TGGAAAGGAC GATACATTAC AGCGACGATC TTTAAAACGC GTTAAGTCga
1621caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)