Construct: ORF TRCN0000477858
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008262.1_s317c1
- Derived from:
- ccsbBroadEn_04983
- DNA Barcode:
- GTCCAGCACCTCTTCTTATCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMTM4 (146223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477858
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146223 | CMTM4 | CKLF like MARVEL transmembr... | NM_181521.3 | 100% | 100% | |
| 2 | human | 146223 | CMTM4 | CKLF like MARVEL transmembr... | XM_017022954.1 | 93.9% | 94.1% | 624_663delinsG |
| 3 | human | 146223 | CMTM4 | CKLF like MARVEL transmembr... | NM_178818.3 | 88.7% | 88.8% | 624_702delinsG |
| 4 | human | 146223 | CMTM4 | CKLF like MARVEL transmembr... | XM_011522882.1 | 86% | 86% | 98_99ins87 |
| 5 | human | 146223 | CMTM4 | CKLF like MARVEL transmembr... | XR_933210.2 | 62.4% | 1_86del;710_998delinsG | |
| 6 | human | 146223 | CMTM4 | CKLF like MARVEL transmembr... | XR_933209.2 | 62.2% | 1_86del;710_1001delinsG | |
| 7 | mouse | 97487 | Cmtm4 | CKLF-like MARVEL transmembr... | NM_153582.5 | 92.3% | 95.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 693
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggagcggc gaggagctgg acggcttcga gggcgaggcc tcgagcacct 121 ccatgatctc gggcgccagc agcccgtacc agcccaccac cgagccggtg agccagcgcc 181 gcgggctggc cggcctgcgc tgcgaccccg actacctgcg cggcgcgctc ggccgcctCA 241 AGGTCGCCCA AGTGATCTTG GCCCTGATTG CATTCATCTG CATAGAGACC ATCATGGCAT 301 GCTCCCCGTG TGAAGGCCTC TACTTTTTTG AGTTTGTGAG CTGCAGTGCG TTTGTGGTGA 361 CTGGCGTCTT GCTGATTATG TTCAGTCTCA ACCTGCACAT GAGGATCCCC CAGATCAACT 421 GGAATCTGAC AGATTTGGTC AACACTGGAC TCAGCGCTTT CCTTTTCTTT ATTGCTTCAA 481 TCGTACTGGC TGCTTTAAAC CATAGAGCCG GAGCAGAAAT TGCTGCCGTG ATATTTGGCT 541 TCTTGGCGAC TGCGGCATAT GCAGTGAACA CATTCCTGGC AGTGCAGAAA TGGAGAGTCA 601 GCGTCCGCCA GCAGAGCACC AATGACTACA TCCGAGCCCG CACGGAGTCC AGGGATGTGG 661 ACAGTCGCCC TGAGATCCAG CGCCTGGACA CGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 841 CCAGCACCTC TTCTTATCTT ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt