Construct: ORF TRCN0000477870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015119.1_s317c1
- Derived from:
- ccsbBroadEn_14023
- DNA Barcode:
- CAGGGTCGGGCAAGAATTGAGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCC2 (9648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XM_006712871.1 | 99.9% | 99.8% | 1426C>A;3097C>G |
| 2 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XM_011512213.2 | 99.2% | 99.1% | 0_1ins33;1393C>A;3064C>G |
| 3 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | NM_181453.4 | 93.9% | 93.8% | 1_303del;1729C>A;3400C>G |
| 4 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XM_006712870.3 | 93.7% | 93.7% | 1_312del;1738C>A;3409C>G |
| 5 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XR_001739080.1 | 78.9% | (many diffs) | |
| 6 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | NR_028063.2 | 69.5% | (many diffs) | |
| 7 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XR_923067.2 | 68.7% | (many diffs) | |
| 8 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XR_001739079.1 | 68.2% | (many diffs) | |
| 9 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XR_001739078.1 | 67% | (many diffs) | |
| 10 | human | 9648 | GCC2 | GRIP and coiled-coil domain... | XM_006712872.3 | 51.4% | 50.7% | (many diffs) |
| 11 | mouse | 70297 | Gcc2 | GRIP and coiled-coil domain... | XM_017314082.1 | 36.8% | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4818
- ORF length:
- 4749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcaagag gttgaggatt ctgtaacaaa gatgggagat gcacataagg 121 agttggaaca atcacatata aactatgtga aagaaattga aaatttgaaa aatgagttga 181 tggcagtacg ttccaaatac agtgaagaca aagctaactt acaaaagcag ctggaagaag 241 caatgaatac gcaattagaa ctttcagaac aacttaaatt tcagaacaac tctgaagata 301 atgttaaaaa actacaagaa gagattgaga aaattaggcc aggctttgag gagcaaattt 361 tatatctgca aaagcaatta gacgctacca ctgatgaaaa gaaggaaaca gttactcaac 421 tccaaaatat cattgaggct aattctcagc attaccaaaa aaatattaat agtttgcagg 481 aagagctttt acagttgaaa gctatacacc aagaagaggt gaaagagttg atgtgccaga 541 ttgaagcatc agctaaggaa catgaagcag agataaataa gttgaacgag ctaaaagaga 601 acttagtaaa acaatgtgag gcaagtgaaa agaacatcca gaagaaatat gaatgtgagt 661 tagaaaattt aaggaaagcc acctcaaatg caaaccaaga caatcagata tgttctattc 721 tcttgcaaga aaatacattt gtagaacaag tagtaaatga aaaagtcaaa cacttagaag 781 ataccttaaa agaacttgaa tctcaacaca gtatcttaaa agatgaggta acttatatga 841 ataatcttaa gttaaaactt gaaatggatg ctcaacatat aaaggatgag ttttttcatg 901 aacgggaaga cttagagttt aaaattaatg aattattact agctaaagaa gaacagggct 961 gtgtaattga aaaattaaaa tctgagctag caggtttaaa taaacagttt tgctatactg 1021 tagaacagca taacagagaa gtacagagtc ttaaggaaca acatcaaaaa gaaatatcag 1081 aactaaatga gacatttttg tcagattcag aaaaagaaaa attaacatta atgtttgaaa 1141 tacagggtct taaggaacag tgtgaaaacc tacagcaaga aaagcaagaa gcaattttaa 1201 attatgagag tttacgagag attatggaaa ttttacaaac agaactgggg gaatctgctg 1261 gaaaaataag tcaagagttc gaatcaatga agcaacagca agcatctgat gttcatgaac 1321 tgcagcagaa gctcagaact gcttttactg aaaaagatgc ccttctcgaa actgtgaatc 1381 gcctccaggg agaaaatgaa aagttactat ctcaacaaga attggtacca gaacttgaaa 1441 ataccataaa gaaccttcaa gaaaagaatg gagtatactt acttagtctc agtaaaagag 1501 ataccatgtt aaaagaatta gaaggaaaga taaattctct tactgaggaa aaagatgatt 1561 ttataaataa actgaaaaat tcccatgaag aaatggataa tttccataag aaatgtgaaa 1621 gggaagaaag attgattctt gaacttggga agaaagtaga gcaaacaatc cagtacaaca 1681 gtgaactaga acaaaaggta aatgaattaa caggaggact agaggagact ttaaaagaaa 1741 aggatcaaaa tgaccaaaaa ctagaaaaac ttatggttca aatgaaagtt ctctctgaag 1801 acaaagaagt attgtcagct gaagtgaagt ctctttatga ggaaaacaat aaactcagtt 1861 cagaaaaaaa acagttgagt agggatttgg aggttttttt gtctcaaaaa gaagatgtta 1921 tccttaaaga acatattact caattagaaa agaaacttca gttaatggtt gaagagcaag 1981 ataatttaaa taaactgctt gaaaatgagc aagttcagaa gttatttgtt aaaactcagt 2041 tgtatggttt tcttaaagaa atgggatcag aagtttcaga agacagtgaa gagaaagatg 2101 ttgttaatgt cctacaggca gtcggtgaat ccttggcaaa aataaatgag gaaaaatgca 2161 acctggcttt tcagcgtgat gaaaaagtat tagagttaga aaaagagatt aagtgccttc 2221 aagaagagag tgtagttcag tgtgaagaac ttaagtcttt attgagagac tatgagcaag 2281 agaaagttct cttaaggaaa gagttagaag aaatacagtc agaaaaagag gccctgcagt 2341 ctgatcttct agaaatgaag aatgctaatg aaaaaacaag gcttgaaaat cagaatcttt 2401 taattcaagt tgaagaagta tctcaaacat gtagcaaaag tgaaatccat aatgaaaaag 2461 aaaaatgttt tataaaggaa catgaaaacc taaagccact actagaacaa aaagaattac 2521 gagataggag agcagagttg atactattaa aggattcctt agcaaaatca ccttctgtaa 2581 aaaatgatcc tctgtcttca gtaaaagagt tggaagaaaa aatagaaaat ctggaaaaag 2641 aatgcaaaga aaaggaggag aaaataaata agataaaatt agttgccgta aaggcaaaga 2701 aagaactaga ttccagcaga aaagagaccc agactgtgaa ggaagaactt gaatctcttc 2761 gatcagaaaa ggaccagtta tctgcttcca tgagagatct cattcaagga gcagaaagct 2821 ataagaatct tttattagaa tatgaaaagc agtcagagca actggatgtg gaaaaagaac 2881 gtgctaataa ttttgagcat cgtattgaag accttacaag acaattaaga aattcgactt 2941 tgcagtgtga aacaataaat tctgataatg aagatctcct ggctcgtatt gagacattac 3001 agtctaatgc caaattatta gaagtacaga ttttagaagt ccagagagcc aaagcaatgg 3061 tagacaaaga attagaagct gaaaaacttc agaaagaaca gaagataaag gaacatgcca 3121 ctactgtaaa tgaacttgaa gaacttcagg tacaacttca aaaggaaaag aaacagcttc 3181 agaaaaccat gcaagaatta gagctggtta aaaaggatgc ccaacaaacc acattgatga 3241 atatggaaat agctgattat gaacgtttga tgaaagaact aaatcaaaag ttaactaata 3301 aaaacaacaa gatagaagat ttggagcaag aaataaaaat tcaaaaacag aaacaagaaa 3361 ccctacaaga agaaataact tcattacagt cttcagtaca acaatatgaa gaaaaaaaca 3421 ccaaaatcaa gcaattgctt gtgaaaacca aaaaggaact ggcagattca aagcaagcag 3481 aaactgatca cttaatactt caagcatctt taaaaggtga gctggaggca agccagcagc 3541 aagtagaagt ctataaaata cagctggctg aaataacatc agagaagcac aaaatccacg 3601 agcacctgaa aacctctgcg gaacagcacc agcgtacgct aagtgcatac cagcagagag 3661 tgacagcact acaggaagag tgccgtgctg ccaaggcaga acaagctact gtaacctctg 3721 aattcgagag ctacaaagtc cgagttcata atgttctaaa acaacagaaa aataaatcta 3781 tgtctcaggc tgaaactgag ggcgctaaac aagaaaggga acatctggaa atgctgattg 3841 accagctaaa aatcaaatta caagatagcc aaaataactt acagattaat gtatctgaac 3901 ttcaaacatt gcagtctgaa catgatacac tgctagaaag gcacaacaag atgctgcagg 3961 aaactgtgtc caaagaggcg gaactccggg aaaaattgtg ttcaatacag tcagagaaca 4021 tgatgatgaa atctgaacat acacagactg tgagtcagct aacatcccag aacgaggtcc 4081 ttcgaaatag cttccgagat caagtgcgac atttgcagga agaacacaga aagacagtgg 4141 agacattaca gcagcagctc tccaagatgg aagcacagct cttccagctt aagaatgaac 4201 cgaccacaag aagcccagtt tcctctcaac aatctttgaa gaaccttcga gaaaGGAGAA 4261 ACACAGACCT CCCGCTTCTA GACATGCACA CTGTAACCCG GGAAGAGGGA GAAGGCATGG 4321 AGACAACTGA TACGGAGTCT GTGTCTTCCG CCAGCACATA CACACAGTCT TTAGAGCAGC 4381 TGCTTAACTC TCCCGAAACT AAACTTGAGC CTCCATTATG GCATGCTGAA TTTACCAAAG 4441 AAGAATTGGT TCAGAAGCTC AGTTCCACCA CAAAAAGTGC AGATCACTTA AACGGCCTGC 4501 TTCGGGAAAC AGAAGCAACC AATGCAATTC TTATGGAGCA AATTAAGCTT CTCAAAAGTG 4561 AAATAAGAAG ATTGGAAAGG AATCAAGAGC GAGAGAAGTC TGCAGCTAAC CTGGAATACT 4621 TGAAGAACGT CTTGCTGCAG TTCATTTTCT TGAAACCAGG TAGTGAAAGA GAGAGACTTC 4681 TTCCTGTTAT AAATACGATG TTGCAGCTCA GCCCTGAAGA AAAGGGAAAA CTTGCTGCGG 4741 TTGCTCAAGG TGAGGAAGAA AATGCTTCCC GTTCTTCTGG ATGGGCATCC TATCTTCATA 4801 GTTGGTCTGG ACTTCGATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 4861 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 4921 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAGGGTC GGGCAAGAAT 4981 TGAGACTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt