Construct: ORF TRCN0000477902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012166.1_s317c1
- Derived from:
- ccsbBroadEn_11040
- DNA Barcode:
- TCACAGCCGGGTCATAGTAAATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMS2P3 (5387)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001242713.3 | 55.5% | 51.4% | (many diffs) |
| 2 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001347879.2 | 55.5% | 51.4% | (many diffs) |
| 3 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001347880.2 | 55.5% | 51.4% | (many diffs) |
| 4 | human | 100533952 | RBAK-RBAKDN | RBAK-RBAKDN readthrough | NM_001204513.3 | 51.8% | 40.7% | (many diffs) |
| 5 | human | 100101440 | PMS2P7 | PMS1 homolog 2, mismatch re... | NR_130940.1 | 37.9% | (many diffs) | |
| 6 | human | 5382 | PMS2P4 | PMS1 homolog 2, mismatch re... | NR_022007.2 | 37.4% | (many diffs) | |
| 7 | human | 5380 | PMS2P2 | PMS1 homolog 2, mismatch re... | NR_003614.3 | 34.8% | (many diffs) | |
| 8 | human | 5382 | PMS2P4 | PMS1 homolog 2, mismatch re... | NR_046299.1 | 34.7% | (many diffs) | |
| 9 | human | 5387 | PMS2P3 | PMS1 homolog 2, mismatch re... | NR_028059.1 | 33.3% | (many diffs) | |
| 10 | human | 5382 | PMS2P4 | PMS1 homolog 2, mismatch re... | NR_046297.1 | 31.9% | (many diffs) | |
| 11 | human | 5383 | PMS2P5 | PMS1 homolog 2, mismatch re... | NR_027776.2 | 21.7% | (many diffs) | |
| 12 | human | 5383 | PMS2P5 | PMS1 homolog 2, mismatch re... | NR_027775.2 | 20.2% | (many diffs) | |
| 13 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322006.2 | 10.2% | 8.7% | (many diffs) |
| 14 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_000535.7 | 9.6% | 8.2% | (many diffs) |
| 15 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322014.2 | 9.5% | 8.1% | (many diffs) |
| 16 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NR_136154.1 | 6.2% | (many diffs) | |
| 17 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | NM_008886.2 | 8.8% | 7.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 576
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacacattg caggggccag tgtcattcaa agatgtggct gtggatttca 121 cccaggagga gtggcggcaa ctggaccctg atgagaagat agcatacggg gatgtgatgt 181 tGGAGAACTA CAGCCATCTA GTTTCTGTGG GGTATGATTA TCACCAAGCC AAACATCATC 241 ATGGAGTGGA GGTGAAGGAA GTGGAGCAGG GAGAGGAGCC GTGGATAATG GAAGGTGAAT 301 TTCCATGTCA ACATAGTCCA GAACCTGCTA AGGCCATCAA ACCTATTGAT CGGAAGTCAG 361 TCCATCAGAT TTGCTCTGGG CCAGTGGTAC TGAGTCTAAG CACTGCAGTG AAGGAGTTAG 421 TAGAAAACAG TCTGGATGCT GGTGCCACTA ATATTGATCT AAAGCTTAAG GACTATGGAG 481 TGGATCTCAT TGAAGTTTCA GACAATGGAT GTGGGGTAGA AGAAGAAAAC TTTGAAGGCT 541 TAACGATGTC ACCATTTCTA CCTGCCACGC GTCGGTTGCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ATCACAGCCG GGTCATAGTA AATTTACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt