Construct: ORF TRCN0000477932
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014597.1_s317c1
- Derived from:
- ccsbBroadEn_09275
- DNA Barcode:
- ATAGCAGGATTCACGGGAACGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRFAM7A (89832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477932
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021884.1 | 99.6% | 100% | 147G>A;390A>G;726C>T |
| 2 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_148911.1 | 99.5% | 100% | (many diffs) |
| 3 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_005254750.3 | 99.5% | 100% | (many diffs) |
| 4 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521177.2 | 79.8% | 80% | (many diffs) |
| 5 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | NM_139320.1 | 77.5% | 77.9% | (many diffs) |
| 6 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522153.2 | 77.5% | 77.9% | (many diffs) |
| 7 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021883.2 | 72.7% | 72.9% | (many diffs) |
| 8 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_017021882.1 | 72.5% | 72.7% | (many diffs) |
| 9 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521176.3 | 65.8% | 66% | (many diffs) |
| 10 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_000746.6 | 63.7% | 63.9% | (many diffs) |
| 11 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NM_001190455.3 | 60.2% | 60.4% | (many diffs) |
| 12 | human | 89832 | CHRFAM7A | CHRNA7 (exons 5-10) and FAM... | XM_011522155.3 | 48% | 33.7% | (many diffs) |
| 13 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | XM_011521178.3 | 39.5% | 27.7% | (many diffs) |
| 14 | human | 1139 | CHRNA7 | cholinergic receptor nicoti... | NR_046324.1 | 30.3% | (many diffs) | |
| 15 | mouse | 11441 | Chrna7 | cholinergic receptor, nicot... | NM_007390.3 | 55.1% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1032
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaggaggca gatatcagtg gctatatccc caatggagaa tgggacctag 121 tgggaatccc cggcaagagg agtgaaaggt tctatgagtg ctgcaaagag ccctaccccg 181 atgtcacctt cacagtgacc atgcgccgca ggacactcta ctatggcctc aacctgctga 241 tcccctgtgt gctcatctcc gccctcgccc tgctggtgtt cctgcttcct gcagattccg 301 gggagaagat ttccctgggg ataacagtct tactctctct taccgtcttc atgctgctcg 361 tggctgagat catgcccgca acatccgatt cggtaccatt gatagcccag tacttcgcca 421 gcaccatgat catcgtgggc ctctcggtgg tggtgacggt gatcgtgctg cagtaccacc 481 accacgaccc cgacgggggc aagatgccca agtggaccag agtcatcctt ctgaactggt 541 gcgcgtggtt cctgcgaatg aagaggcccg gggaggacaa ggtgcgcccg gcctgccagc 601 acaagcagcg gcgctgcagc ctggccagtg tggagatgag cgccgtggcg ccgccgcccg 661 ccagcaacgg gaacctgctg tacatcggct tccgcggcct ggacggcgtg cactgtgtcc 721 cgacccccga ctctggggta gtgtgtggcc gcatggcctg ctcccccacg cacgatgagc 781 acctccTGCA CGGTGGGCAA CCCCCCGAGG GGGACCCGGA CTTGGCCAAG ATCCTGGAGG 841 AGGTCCGCTA CATTGCCAAC CGCTTCCGCT GCCAGGACGA AAGCGAGGCG GTCTGCAGCG 901 AGTGGAAGTT CGCCGCCTGT GTGGTGGACC GCCTGTGCCT CATGGCCTTC TCGGTCTTCA 961 CCATCATCTG CACCATCGGC ATCCTGATGT CGGCTCCCAA CTTCGTGGAG GCCGTGTCCA 1021 AAGACTTTGC GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATA GCAGGATTCA CGGGAACGAA 1201 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t