Construct: ORF TRCN0000477981
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001154.1_s317c1
- Derived from:
- ccsbBroadEn_07803
- DNA Barcode:
- CCTGTTTCCCCCGACCCTGAGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASA3 (22821)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477981
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22821 | RASA3 | RAS p21 protein activator 3 | NM_007368.4 | 99.9% | 100% | 1326T>C |
| 2 | human | 22821 | RASA3 | RAS p21 protein activator 3 | NM_001320822.2 | 96.1% | 96.1% | 0_1ins96;1230T>C |
| 3 | human | 22821 | RASA3 | RAS p21 protein activator 3 | XM_011534841.3 | 96.1% | 96.1% | 0_1ins96;1230T>C |
| 4 | human | 22821 | RASA3 | RAS p21 protein activator 3 | XM_011534842.2 | 96.1% | 96.1% | 0_1ins96;1230T>C |
| 5 | human | 22821 | RASA3 | RAS p21 protein activator 3 | NM_001320821.2 | 54% | 54% | 0_1ins1149;177T>C |
| 6 | human | 22821 | RASA3 | RAS p21 protein activator 3 | XM_017020438.1 | 54% | 54% | 0_1ins1149;177T>C |
| 7 | human | 22821 | RASA3 | RAS p21 protein activator 3 | XM_017020439.1 | 54% | 54% | 0_1ins1149;177T>C |
| 8 | mouse | 19414 | Rasa3 | RAS p21 protein activator 3 | NM_009025.2 | 87.1% | 94.8% | (many diffs) |
| 9 | mouse | 19414 | Rasa3 | RAS p21 protein activator 3 | XM_006508734.1 | 83.7% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2568
- ORF length:
- 2502
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtggaggac gaggggctcc gggtcttcca gagcgtgaag atcaagatcg 121 gtgaagccaa aaaccttccc tcttacccgg ggccgagcaa gatgagggat tgctactgca 181 cggtgaacct ggaccaggag gaggttttca ggaccaaaat tgtggaaaag tcactctgcc 241 cgttttacgg agaagacttt tactgtgaaa ttcctcggag ctttcgtcac ctgtccttct 301 acattttcga tagagacgtt ttccggaggg attccatcat agggaaggtg gccatccaga 361 aggaggactt gcagaagtac cacaacaggg acacctggtt ccagctgcag cacgtggacg 421 ctgactcgga agtgcagggc aaagtgcacc tggagctgcg gctgagcgag gtcatcacag 481 acactggggt cgtctgccac aagctcgcca cacgcatcgt cgagtgccag ggcctcccca 541 tcgtgaatgg gcaatgtgac ccctacgcca ccgtgacgct ggcaggaccc ttcagatcag 601 aagcaaagaa gacgaaagtg aagaggaaga ccaacaatcc ccagttcgat gaagtgtttt 661 attttgaggt gacccggccc tgtagctaca gcaagaagtc ccactttgac tttgaggagg 721 aagacgtgga caagctcgaa atcagagttg acctctggaa tgccagtaac ctgaagtttg 781 gagatgaatt cctgggagaa ctaaggatcc cgttgaaagt cctgcggcag tccagctcct 841 acgaggcgtg gtacttcctc cagccccggg acaatggtag caagagccta aagccagacg 901 acctgggctc cctgcggctg aacgtggtat acacggaaga ccacgtgttt tcttctgact 961 attacagccc tctgcgggac ctgctgttga agtctgcgga tgtggagccc gtgtcagcgt 1021 ctgcggccca catcctgggc gaggtttgcc gggagaagca ggaggcggcc gtcccgctgg 1081 tgcggctctt cctacactat ggcagggtgg tgccattcat cagtgccatc gccagcgcgg 1141 aggtgaagcg gacccaggac cccaacacca tcttccgagg aaactcactg gcgtccaagt 1201 gcatcgacga gaccatgaag ctggcgggga tgcattacct gcatgtcacc ctgaagcccg 1261 ccatcgagga gatatgccag agccacaaac cctgtgaaat cgaccctgtg aagttgaaag 1321 acggagaaaa ccttgaaaac aacatggaga acctacggca gtatgtggac cgcgtcttcc 1381 acgccatcac cgagtctggg gtgagctgcc cgaccgtcat gtgtgacatc ttcttctccc 1441 tccgggaggc ggcggccaag cgcttccagg atgacccgga cgtcaggtac actgcagtga 1501 gcagcttcat cttcctgagg ttctttgcgc ccgccattct ctcccccaac ctcttccagc 1561 tcacgccgca ccacacggac ccccagacgt ccaggacgct gacattgatc tccaagaccg 1621 ttcagaccct cggcagcctg tccaagtcca aatctgcgag ttttaaggag tcctacatgg 1681 ctacatttta tgaattcttc aatgagcaga aatatgctga tgcggtgaag aacttcttgg 1741 atctgatttc gtcctcgggg agaagagacc ccaagagtgt tgagcagccc atcgtgctta 1801 aagaagggtt catgatcaag agggcccaag gacggaagcg ctttgggatg aagaatttta 1861 agaagagatg gtttcgcttg accaaccatg aatttaccta ccacaaaagc aaaggggacc 1921 agcctctcta cagcattccc atcgagaaca tcctggcagt ggagaagctg gaggaggagt 1981 ctttcaaaat gaaaaacatg ttccaggtca tccagccaga gcgtgcgctg tacatccagg 2041 ccaacaactg cgtggaggcc aaggactgga tcgacattct caccaaagtg agccagtgca 2101 accagaagcg cctcaccgtc taccacccgt ccgcctacct gagcggccac tggctgtgct 2161 gtagggcgcc atccgactcg gctccgggct gctcgccctg cactggcggC CTCCCAGCCA 2221 ACATCCAGCT GGACATTGAT GGGGACCGTG AGACGGAGCG TATCTACTCC CTCTTCAACT 2281 TGTACATGAG CAAGCTGGAG AAGATGCAGG AGGCCTGTGG GAGCAAATCT GTGTATGACG 2341 GCCCGGAGCA GGAGGAGTAT TCGACGTTCG TCATTGACGA CCCCCAGGAG ACCTACAAGA 2401 CGCTAAAGCA AGTCATCGCT GGGGTTGGGG CTTTGGAGCA GGAGCACGCC CAGTATAAGA 2461 GGGACAAGTT CAAGAAGACG AAATATGGAA GCCAGGAGCA CCCCATCGGA GACAAGAGCT 2521 TCCAGAACTA CATCCGGCAG CAGTCCGAGA CCTCCACTCA TTCCATTTAC CCAACTTTCT 2581 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2641 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2701 GTGGAAAGGA CGACCTGTTT CCCCCGACCC TGAGTCGACG CGTTAAGTCg acaatcaacc 2761 tctggattac aaaatttgtg aaagatt