Construct: ORF TRCN0000478019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009199.1_s317c1
- Derived from:
- ccsbBroadEn_14277
- DNA Barcode:
- GGCAGTGCACAACGAATTTGCACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TRAPPC13 (80006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80006 | TRAPPC13 | trafficking protein particl... | NM_001243737.1 | 84.5% | 28.6% | 1_189del;545_546insN;1192A>G |
| 2 | human | 80006 | TRAPPC13 | trafficking protein particl... | NM_001093756.1 | 84.3% | 28.7% | (many diffs) |
| 3 | human | 80006 | TRAPPC13 | trafficking protein particl... | NM_001093755.1 | 83.4% | 28.2% | 1_189del;546_563delinsN;1210A>G |
| 4 | human | 80006 | TRAPPC13 | trafficking protein particl... | NM_024941.4 | 83.1% | 28.2% | (many diffs) |
| 5 | human | 80006 | TRAPPC13 | trafficking protein particl... | NM_001365342.1 | 72.9% | 6.8% | 0_1ins282;74_75insN;721A>G |
| 6 | human | 80006 | TRAPPC13 | trafficking protein particl... | NM_001365343.1 | 72.6% | 6.8% | (many diffs) |
| 7 | mouse | 66975 | Trappc13 | trafficking protein particl... | NM_001093760.1 | 74.7% | 28.3% | (many diffs) |
| 8 | mouse | 66975 | Trappc13 | trafficking protein particl... | XM_006517737.3 | 74.5% | 28.4% | (many diffs) |
| 9 | mouse | 66975 | Trappc13 | trafficking protein particl... | NM_025879.2 | 73.7% | 27.9% | (many diffs) |
| 10 | mouse | 66975 | Trappc13 | trafficking protein particl... | NM_001093759.1 | 73.5% | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 429
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgacttta ccacagaatt ttgggaatat atttttggga gagacctttt 121 ccagttatat cagcgttcat aatgatagca atcaagttgt aaaagacata ttagtaaaag 181 ctgatcttca gacaagttct cagcgtttaa atctttcagc ctccaatgct gcagtggctg 241 aacttaaacc ggattgttgt attgatgatg tcatacatca tgaagtcaaa gaaattggaa 301 cacacatctt ggtatgtgct gtgagttata caactcaggc tggagaaaaa atgtatttca 361 gaaaattctt caaatttcag gttctcaaac cattggatgt gaaaaccaaa ttttacaatg 421 cagangactg atgaagtatt tctggaagcc cagattcaga atatgacaac ctcacctatg 481 tttatggaga aggtttcact ggagccatct attatgtaca atgtaacaga attaaattca 541 gtcagccaag ctggagaatg tgtgtctacg tttgggtcaa gagcatattt gcaaccaatg 601 gatacacgcc agtacttata ctgcctaaag CCAAAGAATG AATTTGCAGA AAAAGCAGGC 661 ATCATTAAGG GAGTAACAGT AATTGGAAAA TTGGATATAG TATGGAAAAC AAATCTAGGT 721 GAAAGGGGAA GGTTACAGAC CAGCCAACTT CAAAGAATGG CTCCAGGTTA TGGAGATGTT 781 AGGTTGTCTT TGGAGGCAAT ACCAGATACC GTAAACCTTG AAGAACCTTT TCATATTACC 841 TGTAAAATAA CAAACTGCAG CAGTGAAAGG ACTATGGATC TGGTTTTGGA AATGTGCAAT 901 ACCAATTCCA TCCACTGGTG TGGAATTTCA GGAAGACAGC TGGGAAAGCT GCATCCAAGT 961 TCTTCGCTCT GTCTTGCCCT TACTCTGCTT TCTTCAGTAC AGGGACTGCA AAGCATCTCT 1021 GGCTTAAGAC TAACAGACAC ATTCTTAAAG AGAACATATG AATATGATGA CGTCGCACAA 1081 GTCTGTGTGG TATCTTCTGC CATTAAAGTG GAAAGCTTGC CAACTTTCTT GTACAAAGTG 1141 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 1201 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 1261 GAGGCAGTGC ACAACGAATT TGCACAACGC GTTAAGTCga caatcaacct ctggattaca 1321 aaatttgtga aagatt