Construct: ORF TRCN0000478025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015275.1_s317c1
- Derived from:
- ccsbBroadEn_07421
- DNA Barcode:
- GTACACCGCTTATTTCTGACAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCYT1B (9468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9468 | PCYT1B | phosphate cytidylyltransfer... | NM_001163264.2 | 99.9% | 99.7% | 10C>A |
| 2 | human | 9468 | PCYT1B | phosphate cytidylyltransfer... | NM_004845.5 | 93.1% | 90.8% | (many diffs) |
| 3 | human | 9468 | PCYT1B | phosphate cytidylyltransfer... | NM_001163265.2 | 80% | 78.3% | (many diffs) |
| 4 | human | 9468 | PCYT1B | phosphate cytidylyltransfer... | XM_017029977.1 | 77.7% | 77.7% | 0_1ins234 |
| 5 | mouse | 236899 | Pcyt1b | phosphate cytidylyltransfer... | NM_177546.2 | 86.8% | 93.4% | (many diffs) |
| 6 | mouse | 236899 | Pcyt1b | phosphate cytidylyltransfer... | XM_006527985.3 | 86.7% | 92.3% | (many diffs) |
| 7 | mouse | 236899 | Pcyt1b | phosphate cytidylyltransfer... | NM_211138.1 | 84.5% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1122
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtaggcaat caggagtgca tcatggaaga ggacaatcgc gctccccaat 121 tgtggcgcaa gaccctgact gcacctgccc catttgctga tgaaaccaac tgccagtgtc 181 aagcacccca tgaaaaactg accattgctc aggcccgctt aggaacacca gctgacaggc 241 ctgtcagagt atacgccgat ggaatatttg acctcttcca ctcaggtcat gcaagagccc 301 ttatgcaagc aaaaacactg tttcccaaca gctacttgtt ggtaggagtt tgcagtgatg 361 atctcaccca caaattcaaa ggtttcaccg tgatgaatga agccgagaga tacgaagctc 421 tcagacactg tcgctacgta gacgaagtta tcagagatgc tccctggaca ctcacgccag 481 agtttctgga aaaacacaag attgactttg tggctcatga tgacattccg tattcctctg 541 ctggctctga tgatgtttac aagcacataa aggaagcagg gatgttcgtt ccaacgcaga 601 gaacagaagg catctcaaca tcggacatca ttaccagaat tgttcgtgac tatgatgttt 661 atgcccgacg taacctccag agagggtata cagccaagga actgaatgtc agctttataa 721 atgagaagag gtaccgtttc cagaaccaag tggacaaaat gaaggaaaaa gtcaagaatg 781 tggaggaaag atcaaaggaa tttgtgaaca gagtggaaga aaagagccat gatctaattc 841 aaaagtggga agagaagtca agggaattca ttggcaactt cctagaactg tttggaccTG 901 ATGGAGCATG GAAGCAGATG TTCCAGGAGA GGAGCAGCCG GATGCTGCAG GCCTTATCCC 961 CGAAGCAGAG CCCTGTGAGC AGCCCAACCC GGAGCCGGTC CCCTTCCCGC TCCCCATCGC 1021 CCACCTTCTC ATGGCTTCCA CTCAAAACCT CACCCCCTTC CTCACCCAAA GCAGCCTCAG 1081 CCTCTATCAG CAGCATGAGC GAGGGGGATG AGGATGAAAA GTTGCCAACT TTCTTGTACA 1141 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1201 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1261 AGGACGAGTA CACCGCTTAT TTCTGACAAT AACGCGTTAA GTCgacaatc aacctctgga 1321 ttacaaaatt tgtgaaagat t