Construct: ORF TRCN0000478121
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007077.1_s317c1
- Derived from:
- ccsbBroadEn_10813
- DNA Barcode:
- CATCTAAAGTACTTAAGGTCTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYA1 (2138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478121
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013213.1 | 91.4% | 91.5% | 0_1ins132;1404T>C |
| 2 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013212.2 | 91.1% | 88.1% | (many diffs) |
| 3 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001288575.2 | 90.4% | 90.5% | 0_1ins132;66_67insGGTGCATTGTGGGCA;1389T>C |
| 4 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013211.2 | 89.2% | 86.3% | (many diffs) |
| 5 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_000503.6 | 86.1% | 83.3% | (many diffs) |
| 6 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370334.1 | 86.1% | 83.3% | (many diffs) |
| 7 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370335.1 | 86.1% | 83.3% | (many diffs) |
| 8 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_172058.4 | 86.1% | 83.3% | (many diffs) |
| 9 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_011517483.2 | 85.9% | 82.9% | (many diffs) |
| 10 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013208.2 | 85.9% | 82.9% | (many diffs) |
| 11 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001288574.2 | 85.1% | 82.1% | (many diffs) |
| 12 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370333.1 | 82.1% | 79.4% | (many diffs) |
| 13 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013202.1 | 82.1% | 79.4% | (many diffs) |
| 14 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013203.2 | 81.9% | 79.1% | (many diffs) |
| 15 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_011517484.3 | 81.3% | 78.7% | (many diffs) |
| 16 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013204.2 | 81.1% | 78.3% | (many diffs) |
| 17 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013205.2 | 78.7% | 75.7% | (many diffs) |
| 18 | human | 2138 | EYA1 | EYA transcriptional coactiv... | XM_017013207.2 | 77.3% | 74.7% | (many diffs) |
| 19 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_172059.5 | 76.5% | 73.9% | (many diffs) |
| 20 | human | 2138 | EYA1 | EYA transcriptional coactiv... | NM_001370336.1 | 76.3% | 73.6% | (many diffs) |
| 21 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | NM_001252192.1 | 82.8% | 85.8% | (many diffs) |
| 22 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315414.1 | 82.4% | 89.5% | (many diffs) |
| 23 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315422.1 | 82.4% | 89.5% | (many diffs) |
| 24 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_006495452.3 | 80.5% | 78.3% | (many diffs) |
| 25 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_011238346.2 | 80.5% | 78.3% | (many diffs) |
| 26 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315381.1 | 80.5% | 78.3% | (many diffs) |
| 27 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_011238344.2 | 78.3% | 81.4% | (many diffs) |
| 28 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | NM_001310459.1 | 78.2% | 81.1% | (many diffs) |
| 29 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_017315348.1 | 78.2% | 81.1% | (many diffs) |
| 30 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | NM_010164.2 | 77.5% | 80.6% | (many diffs) |
| 31 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_006495451.3 | 77.4% | 80.3% | (many diffs) |
| 32 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_006495446.3 | 76.1% | 74.2% | (many diffs) |
| 33 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XM_011238347.2 | 63.4% | 65.1% | (many diffs) |
| 34 | mouse | 14048 | Eya1 | EYA transcriptional coactiv... | XR_001780756.1 | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1626
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttccatct aatgcatggg ctttctattt tctgtcattt ctgacaaata 121 gcagaccata cccacatatt ctccctaccc cttcctcaca aactatggct gcatatgggc 181 aaacacagtt taccacagga atgcaacaag ctacagccta tgccacgtac ccacagccag 241 gacagccgta cggcatttcc tcatatggtg cattgtgggc aggcatcaag actgaaggtg 301 gattgtcaca gtctcagtca cctggacaga caggatttct cagctatggc acaagcttca 361 gtacccctca acctggacag gcaccataca gctaccagat gcaaggtagc agttttacaa 421 catcatcagg aatatataca ggaaataatt cactcacaaa ttcctctgga tttaatagtt 481 cacagcagga ctatccgtct tatcccagtt ttggccaggg tcagtacgca cagtattata 541 acagctcacc gtatccagca cattatatga ccagcagcaa caccagccca acgacaccat 601 ccaccaatgc cacttaccag cttcaagaac cgccatctgg catcaccagc caagcagtta 661 cagatcccac agcagagtac agcacaatcc acagcccatc aacacccatt aaagattcag 721 attctgatcg attgcgtcga ggttcagatg ggaaatcacg tggacggggc cgaagaaaca 781 ataatccttc acctccccca gattctgatc ttgagagagt gttcatctgg gacttggatg 841 agacaatcat tgttttccac tccttgctta ctgggtccta cgccaacaga tatgggaggg 901 atccacccac ttcagtttcc cttggactgc gaatggaaga aatgattttc aacttggcag 961 acacacattt attttttaat gacttagaag aatgtgacca agtccatata gatgatgttt 1021 cttcagatga taacggacag gacctaagca catataactt tggaacagat ggctttcctg 1081 ctgcagcaac cagtgctaac ttatgtttgg caactggtgt acggggcggt gtggactgga 1141 tgagaaagtt ggccttccgc tacagacggg taaaagagat ctacaacacc tacaaaaata 1201 atgttggagg tctgcttggt ccagctaaga gggaagcctg gctgcagttg agggccgaaa 1261 ttgaagccct gaccgactcc tggttgacac tggccctgaa agcactctcg ctcattcact 1321 cccggacaaa ctgtgtgaat attttagtaa caactactca gctcatccca gcattggcga 1381 aagtcctgct gtatgggtta ggaattgtat ttccaataga aaatatttac agtGCAACTA 1441 AAATAGGAAA AGAAAGCTGT TTTGAGAGAA TAATTCAAAG GTTTGGAAGA AAAGTGGTGT 1501 ATGTTGTTAT AGGAGATGGT GTAGAAGAAG AACAAGGAGC AAAAAAGCAC GCGATGCCCT 1561 TCTGGAGGAT CTCCAGCCAC TCGGACCTCA TGGCCCTGCA CCACGCCTTG GAACTGGAGT 1621 ACCTGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1681 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1741 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATCTAAAG TACTTAAGGT CTCCAACGCG 1801 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt