Construct: ORF TRCN0000478144
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003599.1_s317c1
- Derived from:
- ccsbBroadEn_08126
- DNA Barcode:
- ACGGGGGCGGTTGAACCTTCTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRHL1 (29841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478144
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29841 | GRHL1 | grainyhead like transcripti... | NM_198182.3 | 99.8% | 99.6% | 1051A>G;1189G>A |
| 2 | human | 29841 | GRHL1 | grainyhead like transcripti... | XM_006711882.3 | 97.9% | 97.6% | 1051A>G;1189G>A;1322_1357del |
| 3 | human | 29841 | GRHL1 | grainyhead like transcripti... | XM_011510343.2 | 90.5% | 90.1% | (many diffs) |
| 4 | human | 29841 | GRHL1 | grainyhead like transcripti... | XM_017003901.1 | 75.4% | 75.2% | 0_1ins453;598A>G;736G>A |
| 5 | human | 29841 | GRHL1 | grainyhead like transcripti... | XM_017003900.1 | 74% | 73.6% | (many diffs) |
| 6 | human | 29841 | GRHL1 | grainyhead like transcripti... | XM_005246159.4 | 69.3% | 58.4% | (many diffs) |
| 7 | human | 29841 | GRHL1 | grainyhead like transcripti... | XM_006711884.4 | 67.9% | 57.1% | (many diffs) |
| 8 | human | 29841 | GRHL1 | grainyhead like transcripti... | XR_001738727.2 | 49.5% | (many diffs) | |
| 9 | human | 29841 | GRHL1 | grainyhead like transcripti... | XR_001738726.2 | 49% | (many diffs) | |
| 10 | human | 29841 | GRHL1 | grainyhead like transcripti... | XR_001738728.2 | 47.5% | (many diffs) | |
| 11 | mouse | 195733 | Grhl1 | grainyhead-like 1 (Drosophila) | NM_001161406.1 | 88% | 94.6% | (many diffs) |
| 12 | mouse | 195733 | Grhl1 | grainyhead-like 1 (Drosophila) | XM_006515016.3 | 87.7% | 94.3% | (many diffs) |
| 13 | mouse | 195733 | Grhl1 | grainyhead-like 1 (Drosophila) | XM_006515017.3 | 81.2% | 87% | (many diffs) |
| 14 | mouse | 195733 | Grhl1 | grainyhead-like 1 (Drosophila) | NM_145890.2 | 75.8% | 80.2% | (many diffs) |
| 15 | mouse | 195733 | Grhl1 | grainyhead-like 1 (Drosophila) | XM_017315004.1 | 55.2% | 57.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1923
- ORF length:
- 1854
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcaccat gacacaggag tacgacaaca aacggccagt gttggttctt cagaatgaag 121 cactttatcc acagcggcgg tcctacacta gtgaggatga ggcctggaaa tccttcctgg 181 aaaaccctct cactgcagcg accaaagcga tgatgagcat caatggagat gaagacagcg 241 ccgctgcgct gggcctgctc tatgactact acaaggttcc aagagagaga aggtcatcaa 301 cagcaaagcc agaggtggag caccctgagc cagatcacag caaaagaaac agcataccaa 361 ttgtgacaga gcagcccctc atctctgctg gagaaaacag agtgcaagta ctgaaaaatg 421 tgccatttaa cattgtcctt ccccatggca accagctggg cattgataag agaggccatc 481 tgacagctcc agatacgaca gtcactgtct ccatagcaac gatgcctacc cactccatca 541 agacagaaac ccagccacat ggcttcgctg tgggaatccc cccagcagtg tatcatcctg 601 agcccactga gcgggtggtg gttttcgatc ggaatctcaa tactgaccag ttcagctctg 661 gtgctcaagc cccaaatgct caaaggcgaa ctccagactc gaccttctca gagaccttca 721 aggaaggcgt tcaggaggtt ttcttcccct cggatctcag tctgcggatg cctggcatga 781 attcagagga ctatgttttt gacagtgttt ctgggaacaa ctttgaatat accctagaag 841 cttcaaaatc acttcgacag aagccaggag acagtaccat gacgtacctg aacaaaggcc 901 agttctatcc catcaccttg aaggaggtga gcagcagtga aggaatccat catcccatca 961 gcaaagttcg aagtgtgatc atggtggttt ttgctgaaga caaaagcaga gaagatcagt 1021 taaggcattg gaagtactgg cactcccggc agcacaccgc taaacaaaga tgcattgaca 1081 tagctgacta taaagaaagc ttcaacacta tcagtaacgt cgaggagatt gcgtataacg 1141 ccatttcctt cacatgggac atcaacgatg aagcaaaggt tttcatctct gtgaactgct 1201 taagcacaga tttctcttcc cagaagggag tgaaggggtt gcctcttaac attcaaattg 1261 atacctatag ttacaacaac cgcagcaaca agcctgtgca ccgggcctac tgccagatca 1321 aggtcttctg tgacaaggga gctgagcgga aaatcaggga tgaagaacga aagcaaagca 1381 aaagaaaagt ttctgatgtt aaagtgccac tgcttccctc tcacaagcga atggatatca 1441 cagttttcaa acccttcatt gatctcgata ctcagcctgt cctcttcatt cctgacgtgc 1501 actttgccaa cttgcagcgg ggcactcatg tccttcccat tgcctctgaa gaattggagg 1561 gtgaaggctc tgtcttgaaa agggggccgt acggcacaga agatgacttt gctgtccctc 1621 cttctaccaa gctggcccgg atagaagaac caaagagagt gctgctctac gttcgaaagg 1681 agtcagaaga agtctttgat gccctGATGC TCAAAACCCC ATCTTTGAAG GGCTTGATGG 1741 AAGCTATCTC AGACAAATAC GATGTTCCCC ATGACAAGAT TGGGAAAATA TTCAAGAAGT 1801 GTAAAAAGGG GATCCTGGTG AACATGGACG ACAACATTGT GAAGCATTAC TCCAATGAGG 1861 ACACCTTCCA GCTGCAGATT GAAGAAGCCG GGGGGTCTTA CAAGCTCACC CTGACGGAGA 1921 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GGGGGCGGTT GAACCTTCTG TAACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt