Construct: ORF TRCN0000478144

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003599.1_s317c1
Derived from:
ccsbBroadEn_08126
DNA Barcode:
ACGGGGGCGGTTGAACCTTCTGTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRHL1 (29841)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478144
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29841 GRHL1 grainyhead like transcripti... NM_198182.3 99.8% 99.6% 1051A>G;1189G>A
2 human 29841 GRHL1 grainyhead like transcripti... XM_006711882.3 97.9% 97.6% 1051A>G;1189G>A;1322_1357del
3 human 29841 GRHL1 grainyhead like transcripti... XM_011510343.2 90.5% 90.1% (many diffs)
4 human 29841 GRHL1 grainyhead like transcripti... XM_017003901.1 75.4% 75.2% 0_1ins453;598A>G;736G>A
5 human 29841 GRHL1 grainyhead like transcripti... XM_017003900.1 74% 73.6% (many diffs)
6 human 29841 GRHL1 grainyhead like transcripti... XM_005246159.4 69.3% 58.4% (many diffs)
7 human 29841 GRHL1 grainyhead like transcripti... XM_006711884.4 67.9% 57.1% (many diffs)
8 human 29841 GRHL1 grainyhead like transcripti... XR_001738727.2 49.5% (many diffs)
9 human 29841 GRHL1 grainyhead like transcripti... XR_001738726.2 49% (many diffs)
10 human 29841 GRHL1 grainyhead like transcripti... XR_001738728.2 47.5% (many diffs)
11 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) NM_001161406.1 88% 94.6% (many diffs)
12 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) XM_006515016.3 87.7% 94.3% (many diffs)
13 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) XM_006515017.3 81.2% 87% (many diffs)
14 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) NM_145890.2 75.8% 80.2% (many diffs)
15 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) XM_017315004.1 55.2% 57.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1923
ORF length:
1854
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttagcaccat gacacaggag tacgacaaca aacggccagt gttggttctt cagaatgaag
121cactttatcc acagcggcgg tcctacacta gtgaggatga ggcctggaaa tccttcctgg
181aaaaccctct cactgcagcg accaaagcga tgatgagcat caatggagat gaagacagcg
241ccgctgcgct gggcctgctc tatgactact acaaggttcc aagagagaga aggtcatcaa
301cagcaaagcc agaggtggag caccctgagc cagatcacag caaaagaaac agcataccaa
361ttgtgacaga gcagcccctc atctctgctg gagaaaacag agtgcaagta ctgaaaaatg
421tgccatttaa cattgtcctt ccccatggca accagctggg cattgataag agaggccatc
481tgacagctcc agatacgaca gtcactgtct ccatagcaac gatgcctacc cactccatca
541agacagaaac ccagccacat ggcttcgctg tgggaatccc cccagcagtg tatcatcctg
601agcccactga gcgggtggtg gttttcgatc ggaatctcaa tactgaccag ttcagctctg
661gtgctcaagc cccaaatgct caaaggcgaa ctccagactc gaccttctca gagaccttca
721aggaaggcgt tcaggaggtt ttcttcccct cggatctcag tctgcggatg cctggcatga
781attcagagga ctatgttttt gacagtgttt ctgggaacaa ctttgaatat accctagaag
841cttcaaaatc acttcgacag aagccaggag acagtaccat gacgtacctg aacaaaggcc
901agttctatcc catcaccttg aaggaggtga gcagcagtga aggaatccat catcccatca
961gcaaagttcg aagtgtgatc atggtggttt ttgctgaaga caaaagcaga gaagatcagt
1021taaggcattg gaagtactgg cactcccggc agcacaccgc taaacaaaga tgcattgaca
1081tagctgacta taaagaaagc ttcaacacta tcagtaacgt cgaggagatt gcgtataacg
1141ccatttcctt cacatgggac atcaacgatg aagcaaaggt tttcatctct gtgaactgct
1201taagcacaga tttctcttcc cagaagggag tgaaggggtt gcctcttaac attcaaattg
1261atacctatag ttacaacaac cgcagcaaca agcctgtgca ccgggcctac tgccagatca
1321aggtcttctg tgacaaggga gctgagcgga aaatcaggga tgaagaacga aagcaaagca
1381aaagaaaagt ttctgatgtt aaagtgccac tgcttccctc tcacaagcga atggatatca
1441cagttttcaa acccttcatt gatctcgata ctcagcctgt cctcttcatt cctgacgtgc
1501actttgccaa cttgcagcgg ggcactcatg tccttcccat tgcctctgaa gaattggagg
1561gtgaaggctc tgtcttgaaa agggggccgt acggcacaga agatgacttt gctgtccctc
1621cttctaccaa gctggcccgg atagaagaac caaagagagt gctgctctac gttcgaaagg
1681agtcagaaga agtctttgat gccctGATGC TCAAAACCCC ATCTTTGAAG GGCTTGATGG
1741AAGCTATCTC AGACAAATAC GATGTTCCCC ATGACAAGAT TGGGAAAATA TTCAAGAAGT
1801GTAAAAAGGG GATCCTGGTG AACATGGACG ACAACATTGT GAAGCATTAC TCCAATGAGG
1861ACACCTTCCA GCTGCAGATT GAAGAAGCCG GGGGGTCTTA CAAGCTCACC CTGACGGAGA
1921TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1981TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2041GGCTTTATAT ATCTTGTGGA AAGGACGAAC GGGGGCGGTT GAACCTTCTG TAACGCGTTA
2101AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)