Construct: ORF TRCN0000478313

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF011388.1_s317c1
• Derived from: ccsbBroadEn_09098
• DNA Barcode: AGCACCATTAGGCTCGGACTTCCG
• Epitope Tag: V5 (not translated due to frame shift)
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: KATNAL2 (83473)

Vector Information:

• Vector Backbone: pLX_317
• Pol II Cassette 1: SV40-PuroR
• Pol II Cassette 2: EF1a-TRCN0000478313
• Selection Marker: PuroR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	83473	KATNAL2	katanin catalytic subunit A...	NM_031303.3	99.8%	99.5%	263G>A;1397T>A
2	human	83473	KATNAL2	katanin catalytic subunit A...	XM_017026030.2	99.6%	99.3%	70_71insCAG;260G>A;1394T>A
3	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353903.1	91.4%	91.2%	0_1ins117;146G>A;1280T>A
4	human	83473	KATNAL2	katanin catalytic subunit A...	XM_005258361.3	91.4%	91.2%	0_1ins117;146G>A;1280T>A
5	human	83473	KATNAL2	katanin catalytic subunit A...	XM_017026031.1	91.4%	91.2%	0_1ins117;146G>A;1280T>A
6	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001367621.1	86.4%	86.2%	1_216del;479G>A;1613T>A
7	human	83473	KATNAL2	katanin catalytic subunit A...	XM_006722554.4	86.4%	86.2%	1_216del;479G>A;1613T>A
8	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353904.1	84.4%	84.1%	(many diffs)
9	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353899.1	82.5%	82.2%	1_294del;557G>A;1691T>A
10	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353900.1	82.3%	82%	(many diffs)
11	human	83473	KATNAL2	katanin catalytic subunit A...	XM_017026028.2	71%	68.2%	(many diffs)
12	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353905.1	69.5%	65%	(many diffs)
13	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353906.1	69.5%	65%	(many diffs)
14	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353907.1	69.2%	64.9%	(many diffs)
15	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353901.1	67.5%	63.6%	(many diffs)
16	human	83473	KATNAL2	katanin catalytic subunit A...	XM_011526219.3	66.3%	56.2%	(many diffs)
17	human	83473	KATNAL2	katanin catalytic subunit A...	XM_011526220.2	64.5%	60.7%	(many diffs)
18	human	83473	KATNAL2	katanin catalytic subunit A...	XM_011526221.3	63.9%	60.3%	(many diffs)
19	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353908.1	62.7%	58.1%	(many diffs)
20	human	83473	KATNAL2	katanin catalytic subunit A...	XM_011526223.3	61.1%	59.1%	(many diffs)
21	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353902.1	58.9%	54.9%	(many diffs)
22	human	83473	KATNAL2	katanin catalytic subunit A...	NM_001353909.1	58.5%	56.1%	(many diffs)
23	human	83473	KATNAL2	katanin catalytic subunit A...	NR_148563.1	32.8%		(many diffs)
24	human	83473	KATNAL2	katanin catalytic subunit A...	XR_001753286.2	21.4%		(many diffs)
25	human	83473	KATNAL2	katanin catalytic subunit A...	XR_002958187.1	18.9%		(many diffs)
26	mouse	71206	Katnal2	katanin p60 subunit A-like 2	XM_011247073.1	75.4%	76.9%	(many diffs)
27	mouse	71206	Katnal2	katanin p60 subunit A-like 2	XM_006526438.3	72.9%	74.5%	(many diffs)
28	mouse	71206	Katnal2	katanin p60 subunit A-like 2	XM_006526437.3	72.8%	74.2%	(many diffs)
29	mouse	71206	Katnal2	katanin p60 subunit A-like 2	NM_027721.2	52.6%	53.5%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1464

ORF length:

1398

Sequence:

1	tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61	ttggcatgga atatgagagt tattattttg taaaatttca gaaatacccc aaaattgtca
121	aaaagtcatc agacacagca gaaaataatt taccgcaaag aagtagaggg aagaccagaa
181	ggatgatgaa cgacagttgt caaaatcttc ccaagatcaa tcagcagagg ccccggtcca
241	aaaccacagc ggggaagaca ggggacacca aatcgctcaa taaggagcat cctaatcagg
301	aggtagttga taacactcgc ctggaaaatg ccaacttcgg cctacatata tcaagaatcc
361	gtaaagacag tggagaggaa aatgcccacc cacgaagagg ccaaatcatt gacttccaag
421	ggctgctcac agatgccatc aagggagcaa ccagtgaact tgccttgaac accttcgacc
481	ataatccaga cccctcagaa cgactgctga aacctctgag tgcatttatt ggcatgaaca
541	gtgagatgcg agaattggca gccgtggtga gccgggacat ttatctccat aatccaaaca
601	taaagtggaa tgacattatt ggacttgatg cagccaagca gttagtcaaa gaagctgttg
661	tgtatcctat aaggtatcca cagctattta caggaattct ttctccctgg aaaggactac
721	tgctgtacgg ccctccaggt acaggaaaga ctttactggc caaagctgtg gccactgaat
781	gtaaaacaac cttctttaac atttctgcat ccaccattgt cagcaaatgg agaggggatt
841	cagaaaaact cgttcgggtg ttatttgagc ttgcccgcta ccacgcccca tccacgatct
901	tcctggacga gctggagtcg gtgatgagtc agagaggcac agcttctggg ggagaacatg
961	aaggaagcct gcggatgaag acagagttac tggtgcagat ggatgggctg gcacgctcag
1021	aagatctcgt atttgtctta gcagcttcta acctgccgtg ggagctggac tgtgccatgt
1081	tacgccgcct ggagaagagg attctggtcg atctccccag ccgggaggcc aggcaggcca
1141	tgatctacca ctggctgcct cctgtgagca agagcagggc cttGGAGCTG CACACAGAGC
1201	TGGAGTACAG TGTGCTGAGC CAGGAGACTG AGGGCTACTC AGGCTCAGAT ATTAAGCTCG
1261	TCTGCAGGGA AGCAGCCATG CGGCCCGTGA GGAAGATCTT TGATGCACTT GAAAATCACC
1321	AGTCAGAAAG CAGCGACTTA CCCAGGATCC AGTTGGATAT AGTAACCACT GCCGACTTTC
1381	TGGATGTGCT AACTCACACC AAGCCCTCCG CAAAGAATCT GGCTCAGAGA TACTCAGACT
1441	GGCAAAGAGA GTTCGAGTCT GACAGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA
1501	GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT
1561	TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG CACCATTAGG
1621	CTCGGACTTC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga
1681	tt

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