Transcript: Human NM_031303.3

Homo sapiens katanin catalytic subunit A1 like 2 (KATNAL2), transcript variant 5, mRNA.

Source:
NCBI, updated 2018-12-12
Taxon:
Homo sapiens (human)
Gene:
KATNAL2 (83473)
Length:
2877
CDS:
222..1622

Additional Resources:

NCBI RefSeq record:
NM_031303.3
NBCI Gene record:
KATNAL2 (83473)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_031303.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000117304 CGCTCAGAAGATCTCGTATTT pLKO.1 1170 CDS 100% 13.200 18.480 N KATNAL2 n/a
2 TRCN0000117306 GTTGTGTATCCTATAAGGTAT pLKO.1 813 CDS 100% 4.950 6.930 N KATNAL2 n/a
3 TRCN0000090749 CGGGACATTTATCTCCATAAT pLKO.1 729 CDS 100% 13.200 9.240 N Katnal2 n/a
4 TRCN0000117302 GATTAACTTGAGCCACTGTAT pLKO.1 1740 3UTR 100% 4.950 3.465 N KATNAL2 n/a
5 TRCN0000117305 GCTCAATAAGGAGCATCCTAA pLKO.1 431 CDS 100% 4.950 3.465 N KATNAL2 n/a
6 TRCN0000117303 CCACAGCTATTTACAGGAATT pLKO.1 834 CDS 100% 0.000 0.000 N KATNAL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_031303.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09098 pDONR223 100% 99.9% 99.7% None 263G>A n/a
2 ccsbBroad304_09098 pLX_304 0% 99.9% 99.7% V5 263G>A n/a
3 TRCN0000478313 AGCACCATTAGGCTCGGACTTCCG pLX_317 20.4% 99.8% 99.5% V5 (not translated due to frame shift) 263G>A;1397T>A n/a
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