Construct: ORF TRCN0000478326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005826.1_s317c1
- Derived from:
- ccsbBroadEn_07850
- DNA Barcode:
- GAACCCGCTGGAAAAGCGTACGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYFIP1 (23191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001287810.4 | 99.9% | 100% | 918G>A |
| 2 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324120.2 | 99.9% | 100% | 918G>A |
| 3 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324123.3 | 99.9% | 100% | 918G>A |
| 4 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_014608.6 | 99.9% | 100% | 918G>A |
| 5 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324124.3 | 97.5% | 97.6% | 116_117ins90;828G>A |
| 6 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324119.2 | 97.3% | 97.3% | 1_102del;1020G>A |
| 7 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324126.3 | 97.2% | 97.2% | 282_283ins102;816G>A |
| 8 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324125.3 | 90.2% | 90.2% | 0_1ins366;552G>A |
| 9 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001033028.2 | 60.4% | 56% | (many diffs) |
| 10 | human | 23191 | CYFIP1 | cytoplasmic FMR1 interactin... | NM_001324122.3 | 55.3% | 55.3% | 0_1ins1680 |
| 11 | mouse | 20430 | Cyfip1 | cytoplasmic FMR1 interactin... | NM_001164661.1 | 90.2% | 99% | (many diffs) |
| 12 | mouse | 20430 | Cyfip1 | cytoplasmic FMR1 interactin... | NM_011370.3 | 90.2% | 99% | (many diffs) |
| 13 | mouse | 20430 | Cyfip1 | cytoplasmic FMR1 interactin... | XM_006540729.2 | 90.2% | 99% | (many diffs) |
| 14 | mouse | 20430 | Cyfip1 | cytoplasmic FMR1 interactin... | NM_001164662.1 | 88.1% | 96.4% | (many diffs) |
| 15 | mouse | 76884 | Cyfip2 | cytoplasmic FMR1 interactin... | NM_001252459.1 | 79.2% | 87.4% | (many diffs) |
| 16 | mouse | 76884 | Cyfip2 | cytoplasmic FMR1 interactin... | NM_001252460.1 | 79.2% | 87.4% | (many diffs) |
| 17 | mouse | 76884 | Cyfip2 | cytoplasmic FMR1 interactin... | NM_133769.3 | 79.2% | 87.4% | (many diffs) |
| 18 | mouse | 76884 | Cyfip2 | cytoplasmic FMR1 interactin... | XM_006534461.3 | 77.6% | 85.7% | (many diffs) |
| 19 | mouse | 76884 | Cyfip2 | cytoplasmic FMR1 interactin... | XM_006534462.3 | 77.6% | 85.7% | (many diffs) |
| 20 | mouse | 76884 | Cyfip2 | cytoplasmic FMR1 interactin... | XM_006534463.2 | 77.6% | 85.7% | (many diffs) |
| 21 | mouse | 20430 | Cyfip1 | cytoplasmic FMR1 interactin... | XM_017322067.1 | 50% | 54.6% | (many diffs) |
| 22 | mouse | 20430 | Cyfip1 | cytoplasmic FMR1 interactin... | XR_001785497.1 | 46.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3825
- ORF length:
- 3759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcccaggtg actctggagg acgcgctgtc caacgtggac ctcctggagg 121 agctgcccct gcccgaccag cagccctgca tcgagccccc gccatcctcg ctgctctacc 181 agccaaattt caacactaac tttgaagaca gaaatgcatt tgttactggc atcgcaagat 241 acattgaaca agccaccgtc cactctagca tgaacgagat gctggaggag ggccaagaat 301 atgctgtcat gctgtacacc tggaggagct gctcccgggc catcccacag gtgaaatgta 361 acgagcagcc taacagagtg gaaatctacg agaaaaccgt ggaggttctg gagcctgagg 421 tcacaaaact gatgaatttc atgtacttcc agagaaatgc cattgagcgt ttctgcgggg 481 aagtgaggcg cctgtgccat gccgagagga ggaaggactt cgtgtcagaa gcctacctga 541 tcacactggg caaattcatc aacatgttcg ctgtgctgga cgagctgaag aacatgaagt 601 gcagtgtgaa gaacgaccac tcagcgtaca agagggccgc tcagttttta cgtaaaatgg 661 cagatccaca gtccatccag gaatcgcaga atctgtccat gttcctggcc aatcataaca 721 agatcacaca gtctctgcag cagcagctcg aagtgatttc tggctacgaa gagctcctgg 781 cagatattgt gaatctgtgt gtggattact acgagaacag gatgtatttg acgcccagtg 841 agaaacacat gcttctcaaa gtcatgggat ttggtctgta cctgatggat gggagtgtca 901 gtaacatcta taagttggat gccaagaaaa gaataaactt atccaaaatc gacaagtact 961 tcaagcaact ccaggtggtt ccactatttg gggacatgca aatagaactg gcaagatata 1021 tcaagaccag cgcccactac gaggaaaata aatctcgatg gacgtgcaca tcctccggca 1081 gcagccctca gtacaacatc tgcgagcaga tgatccagat ccgcgaggac cacatgcgct 1141 tcatttcgga gctggcgcgc tacagcaaca gcgaggtggt cacgggctcg ggccgccagg 1201 aggcccagaa gacggacgcg gagtaccgca agctcttcga cctggcgctg cagggcctgc 1261 agctgttgtc gcagtggagc gcgcacgtga tggaagtgta ttcctggaag cttgtgcacc 1321 ccaccgacaa gtactccaac aaggactgcc ccgacagcgc tgaagagtac gagcgtgcca 1381 cgcgctacaa ctacaccagc gaggagaagt ttgccctagt ggaggtgatc gccatgatca 1441 aaggcctgca ggtgctgatg ggcaggatgg agagcgtgtt caaccacgcc atccggcaca 1501 ccgtctatgc cgcactgcag gacttctccc aggtgaccct tagggagccg ctgcggcagg 1561 ccatcaagaa gaagaagaac gtcatccaga gtgtcctgca ggccatcagg aagaccgtgt 1621 gtgactggga gacggggcat gagcccttca atgacccagc cttgcggggc gagaaggacc 1681 ccaagagcgg cttcgacata aaagtaccac gccgcgccgt gggaccctcc agcactcagc 1741 tttacatggt gagaaccatg ctagagtccc tcattgcaga caaaagtggt tccaagaaaa 1801 ccttgagaag tagccttgag gggcccacca tattggacat agaaaaattt catcgagagt 1861 cattcttcta cactcacttg ataaatttca gtgaaacgct gcagcagtgc tgtgaccttt 1921 cgcagctgtg gttccgagag ttcttcctgg agctgaccat gggcaggagg atccagttcc 1981 ccattgagat gtcgatgccc tggatcctga cggaccacat cctggagacc aaggaggcat 2041 cgatgatgga gtacgtgctc tactccctgg acctgtacaa tgacagcgcc cactacgcgc 2101 tcaccaggtt caacaagcag ttcctgtacg acgaaattga ggccgaggtg aatctatgtt 2161 ttgaccaatt tgtttacaag ctagcagacc agatatttgc ctattataag gttatggcag 2221 gaagtttgct tcttgataaa cggttacgat cagaatgcaa gaatcaggga gccacgatcc 2281 acctcccgcc gtctaaccgc tacgagacgc tgctgaagca gaggcatgtg cagctcctcg 2341 gcagatcaat agacctcaat cgtctgatca cccagcgcgt ctcagcagcc atgtataagt 2401 ccctagaact ggcgattgga cgatttgaaa gtgaagattt gacctccata gttgagctgg 2461 atggcctgtt ggaaatcaac cgcatgaccc acaagctgct gagccggtac ctgacgctgg 2521 acggcttcga cgccatgttc cgggaggcca accacaacgt gtcagcgccc tacgggagga 2581 tcaccctgca cgtcttctgg gagctcaact atgacttcct gcccaactac tgctacaacg 2641 gctctaccaa ccggtttgtt cggacagtgt taccattttc tcaggaattt caaagagata 2701 agcagcctaa tgcacagcct cagtatctgc atggatccaa ggctttgaac ttggcctact 2761 ccagcattta cggcagctac cggaacttcg tgggacctcc acactttcaa gtcatctgcc 2821 ggcttctcgg ctaccagggt atcgccgtgg tcatggagga gctgctgaag gtcgtcaaga 2881 gcctgctgca aggcacaatc ctgcagtacg tgaagacgct gatggaggtg atgcccaaga 2941 tctgccgcct gccccggcac gagtacggct ctcctggtat cctggagttc ttccaccacc 3001 agctgaagga catcgtggag tacgcagagc tgaagacggt gtgcttccag aacctgcggg 3061 aggtggggaa cgccatcctc ttctgcctgc tcatcgagca gagcctgtct ttagaagaag 3121 tgtgtgacct gctgcacgcg gctcctttcc agaacatctt gccgcgagtc catgtgaaag 3181 agggggagag acttgatgcc aaaatgaaaa gactagaatc aaagtacgcc ccgctgcatc 3241 ttgtcccact gattgaaaga ctggggaccc ctcagcaaat tgccatcgca agagaggggg 3301 acctgctgac aaaggagcgc ctctgctgcg gcctgtccat gtttgaggtc atcctgacac 3361 ggatccggag ctttctggat gaccccatct ggcgcgggcc tctgcccagc aatggggtca 3421 tgcatgtgga cgagtgtgtg gagtttcaca gactgtggag tgccatgcag tttgtctact 3481 gcattcccgt ggggacacac gagttcacag tcgagcagtg ctttggtgat gggctacact 3541 gggctggctg tatgaTCATC GTACTTCTTG GGCAGCAGCG GCGTTTTGCT GTGCTGGATT 3601 TCTGCTACCA TCTACTTAAA GTCCAGAAAC ATGATGGCAA AGATGAGATT ATTAAAAATG 3661 TGCCTTTGAA GAAGATGGTG GAGAGAATTC GCAAGTTCCA GATTCTCAAT GATGAGATCA 3721 TCACCATCCT GGATAAGTAC CTGAAGTCAG GCGACGGGGA GGGCACGCCA GTGGAGCATG 3781 TGCGCTGCTT CCAGCCGCCC ATCCACCAGT CCCTCGCCAG CAGCTGCCCA ACTTTCTTGT 3841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3961 GAAAGGACGA GAACCCGCTG GAAAAGCGTA CGGAACGCGT TAAGTCgaca atcaacctct 4021 ggattacaaa atttgtgaaa gatt