Construct: ORF TRCN0000478329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017822.1_s317c1
- Derived from:
- ccsbBroadEn_03302
- DNA Barcode:
- ATCGTGTAATAAACAACTTGGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTL6B (51412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51412 | ACTL6B | actin like 6B | NM_016188.5 | 100% | 100% | |
| 2 | human | 51412 | ACTL6B | actin like 6B | NR_134539.2 | 82.9% | 1_94del;1207_1223del;1390_1541del | |
| 3 | mouse | 83766 | Actl6b | actin-like 6B | NM_031404.4 | 92.3% | 99.7% | (many diffs) |
| 4 | mouse | 83766 | Actl6b | actin-like 6B | XM_006504635.2 | 90.6% | 97.9% | (many diffs) |
| 5 | mouse | 83766 | Actl6b | actin-like 6B | XM_006504636.3 | 72.6% | 79.4% | (many diffs) |
| 6 | mouse | 83766 | Actl6b | actin-like 6B | XM_006504637.3 | 72.6% | 79.4% | (many diffs) |
| 7 | mouse | 83766 | Actl6b | actin-like 6B | XM_006504638.3 | 60.5% | 66.8% | (many diffs) |
| 8 | mouse | 83766 | Actl6b | actin-like 6B | XM_017321187.1 | 60.5% | 66.8% | (many diffs) |
| 9 | mouse | 83766 | Actl6b | actin-like 6B | XM_006504639.3 | 55.2% | 54.7% | (many diffs) |
| 10 | mouse | 83766 | Actl6b | actin-like 6B | XM_006504640.3 | 55.2% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1344
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgggggcgtc tacggcggag atgaggtggg ggcgctggtc tttgacattg 121 gctccttctc agtccgcgct gggtacgctg gggaggactg tcccaaggct gacttcccca 181 ccacagtggg gctgctggcc gcggaggagg ggggcgggct ggagctggag ggggacaaag 241 agaagaaagg gaagatcttc cacatcgaca ccaatgccct gcacgtgcct cgggatggag 301 cggaggtcat gtcgcccctc aagaatggca tgatcgagga ctgggagtgc ttccgagcca 361 tcctggatca cacctacagc aaacacgtca agtctgagcc aaacctgcac ccagtgctca 421 tgtccgaggc tccgtggaac acacgggcca agcgggagaa gctgacagag ctgatgttcg 481 agcagtacaa cattcctgcc ttcttcttat gcaagacggc tgtgctcacc gcctttgcaa 541 acgggcggtc cactggcctc gtgctggaca gtggagccac ccacaccacg gccattccag 601 tacatgacgg ctacgttctg cagcaaggca tcgtcaagtc ccctctggca ggggacttca 661 tctccatgca gtgccgggag ctgttccagg agatggccat tgacatcatc ccaccttaca 721 tgatcgcagc caaggagcct gtccgggagg gtgccccccc aaactggaag aagaaggaga 781 agctacccca ggtctccaag tcctggcata actacatgtg taatgaggtg atccaggact 841 tccaggcctc cgtgctgcag gtctcagact ccccctacga tgaacaggtg gctgcacaaa 901 tgcccacagt gcactacgag atgcccaatg gctacaatac agactacggc gccgagcgac 961 tccgcatccc tgagggcctg tttgatccct cgaacgtcaa gggcctgtcg gggaacacca 1021 tgttGGGTGT GGGCCACGTG GTGACCACCA GCATCGGCAT GTGTGACATT GATATTCGCC 1081 CGGGCCTGTA CGGGAGTGTC ATTGTCACCG GCGGGAACAC ACTGCTGCAG GGCTTCACTG 1141 ACAGGCTCAA TCGAGAGCTT TCCCAGAAGA CCCCACCGAG CATGCGACTG AAACTCATTG 1201 CCAGCAACAG CACCATGGAG CGCAAGTTCA GCCCCTGGAT CGGGGGTTCC ATCCTGGCCT 1261 CACTGGGCAC TTTCCAGCAG ATGTGGATCT CCAAGCAGGA ATATGAGGAG GGCGGGAAGC 1321 AGTGCGTGGA GCGAAAGTGC CCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCGTGTAATA 1501 AACAACTTGG TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att