Construct: ORF TRCN0000478413
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004595.2_s317c1
- Derived from:
- ccsbBroadEn_09809
- DNA Barcode:
- TATGTCCAACATCTCACTTAAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHFR2 (200895)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478413
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200895 | DHFR2 | dihydrofolate reductase 2 | NM_001195643.2 | 99.8% | 100% | 240C>A |
| 2 | human | 200895 | DHFR2 | dihydrofolate reductase 2 | NM_176815.5 | 99.8% | 100% | 240C>A |
| 3 | human | 200895 | DHFR2 | dihydrofolate reductase 2 | XM_011512537.3 | 99.8% | 100% | 240C>A |
| 4 | human | 1719 | DHFR | dihydrofolate reductase | NM_000791.4 | 94.6% | 91.9% | (many diffs) |
| 5 | human | 1719 | DHFR | dihydrofolate reductase | NM_001290354.2 | 68.6% | 66.3% | (many diffs) |
| 6 | human | 1719 | DHFR | dihydrofolate reductase | NM_001290357.2 | 65.5% | 61.4% | (many diffs) |
| 7 | human | 1720 | DHFRP3 | dihydrofolate reductase pse... | NR_033423.1 | 51.9% | (many diffs) | |
| 8 | mouse | 13361 | Dhfr | dihydrofolate reductase | NM_010049.3 | 87% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcttttgcta aactgcatcg tcgctgtgtc ccaaaacatg ggcatcggca 121 agaacgggga cctgcccagg ccgccgctca ggaatgaatt caggtatttc cagagaatga 181 ccacaacttc ttcagtagag ggtaaacaga atctggtgat tatgggtagg aagacctggt 241 tctccattcc TGAGAAGAAT CGACCTTTAA AGGATAGAAT TAATTTAGTT CTCAGCAGAG 301 AACTAAAGGA ACCTCCACAA GGAGCTCATT TTCTTGCCAG AAGTTTGGAT GATGCCTTAA 361 AACTTACTGA ACGACCAGAA TTAGCAAATA AAGTAGACAT GATTTGGATA GTTGGTGGCA 421 GTTCTGTTTA TAAGGAAGCC ATGAATCACC TAGGCCATCT TAAACTATTT GTGACAAGGA 481 TCATGCAGGA CTTTGAAAGT GACACGTTTT TTTCAGAAAT TGACTTGGAG AAATATAAAC 541 TTCTGCCTGA ATACCCAGGT GTTCTCTCTG ATGTCCAGGA GGGGAAACAC ATCAAGTACA 601 AATTTGAAGT ATGTGAGAAG GATGATTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATATGTCCA 781 ACATCTCACT TAAACTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt