Construct: ORF TRCN0000478553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009901.1_s317c1
- Derived from:
- ccsbBroadEn_05703
- DNA Barcode:
- TTTATTGTGCGATTTATCCGATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C15 (554313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478553
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 100% | 100% | |
| 2 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 100% | 100% | |
| 3 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 85.8% | 100% | (many diffs) |
| 4 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 85.8% | 100% | (many diffs) |
| 5 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 85.4% | 100% | (many diffs) |
| 6 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 84.4% | 100% | (many diffs) |
| 7 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 84.1% | 100% | (many diffs) |
| 8 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 84.1% | 100% | (many diffs) |
| 9 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 83.1% | 100% | (many diffs) |
| 10 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 82.8% | 100% | (many diffs) |
| 11 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 82.2% | 100% | (many diffs) |
| 12 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 81.2% | 100% | (many diffs) |
| 13 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 80.9% | 100% | (many diffs) |
| 14 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 88.3% | 100% | (many diffs) |
| 15 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 87% | 100% | (many diffs) |
| 16 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 87% | 100% | (many diffs) |
| 17 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 86.4% | 100% | (many diffs) |
| 18 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 86.4% | 100% | (many diffs) |
| 19 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 86.1% | 100% | (many diffs) |
| 20 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 85.8% | 100% | (many diffs) |
| 21 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 85.8% | 100% | (many diffs) |
| 22 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 85.4% | 100% | (many diffs) |
| 23 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 85.4% | 100% | (many diffs) |
| 24 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 84.8% | 100% | (many diffs) |
| 25 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 84.7% | 100% | (many diffs) |
| 26 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 84.7% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cggcagagga aagggcggaa aaggcttagg caaagggggc gcTAAGCGCC 121 ACCGCAAGGT CTTGAGAGAC AACATTCAGG GCATCACCAA GCCTGCCATT CGGCGTCTAG 181 CTCGGCGTGG CGGCGTTAAG CGGATCTCTG GCCTCATTTA CGAGGAGACC CGCGGTGTGC 241 TGAAGGTGTT CCTGGAGAAT GTGATTCGGG ACGCAGTCAC CTACACCGAG CACGCCAAGC 301 GCAAGACCGT CACAGCCATG GATGTGGTGT ACGCGCTCAA GCGCCAGGGG CGCACCCTGT 361 ACGGCTTCGG AGGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTTATTGTGC GATTTATCCG 541 ATTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt