Construct: ORF TRCN0000478631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003678.2_s317c1
- Derived from:
- ccsbBroadEn_03626
- DNA Barcode:
- TTTCTACTTTGAACGTAGCCTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFN1 (55669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478631
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55669 | MFN1 | mitofusin 1 | NM_033540.3 | 100% | 100% | |
| 2 | human | 55669 | MFN1 | mitofusin 1 | XM_005247596.4 | 100% | 100% | |
| 3 | human | 55669 | MFN1 | mitofusin 1 | XM_011512963.3 | 80.1% | 80.1% | 0_1ins441 |
| 4 | mouse | 67414 | Mfn1 | mitofusin 1 | NM_024200.4 | 86.6% | 90.5% | (many diffs) |
| 5 | mouse | 67414 | Mfn1 | mitofusin 1 | XR_001783736.1 | 63.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2289
- ORF length:
- 2223
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaacctgtt tctccactga agcactttgt gctggctaag aaggcgatta 121 ctgcaatctt tgaccagtta ctggagtttg ttactgaagg atcacatttt gttgaagcaa 181 catataagaa tccggaactt gatcgaatag ccactgaaga tgatctggta gaaatgcaag 241 gatataaaga caagctttcc atcattggtg aggtgctatc tcggagacac atgaaggtgg 301 cattttttgg caggacaagc agtgggaaga gctctgttat caatgcaatg ttgtgggata 361 aagttctccc tagtgggatt ggccatataa ccaattgctt cctaagtgtt gaaggaactg 421 atggagataa agcctatctt atgacagaag gatcagatga aaaaaagagt gtgaagacag 481 ttaatcaact ggcccatgcc cttcacatgg acaaagattt gaaagctggc tgtcttgtac 541 gtgtgttttg gccaaaagca aaatgtgccc tcttgagaga tgacctggtg ttagtagaca 601 gtccaggcac agatgtcact acagagctgg atagctggat tgataagttt tgcctagatg 661 ctgatgtctt tgttttggtc gcaaactctg aatcaacact aatgaatacg gaaaaacact 721 tttttcacaa ggtgaatgag cggctttcca agcctaatat tttcattctc aataatcgtt 781 gggatgcctc tgcatcagag ccagaatata tggaagacgt acgcagacag cacatggaaa 841 gatgcctgca tttcttggtg gaggagctca aagttgtaaa tgctttagaa gcacagaatc 901 gtatcttctt tgtttcagca aaggaagttc ttagtgctag aaagcaaaaa gcacagggga 961 tgccagaaag tggtgtggca cttgctgaag gatttcatgc aagattacag gaatttcaga 1021 attttgaaca aatctttgag gagtgtatct cgcagtcagc agtgaaaaca aagttcgaac 1081 agcacactat cagagctaaa cagatactag ctactgtgaa aaacataatg gattcagtaa 1141 acctggcagc tgaagataaa aggcattatt cagtggaaga gagggaagac caaattgata 1201 gactggactt tattcgaaac cagatgaacc ttttaacact ggatgttaag aaaaaaatca 1261 aggaggttac cgaggaggtg gcaaacaaag tttcatgtgc aatgacagat gaaatttgtc 1321 gactgtctgt tttggttgat gaattttgtt cagagtttca tcctaatcca gatgtattaa 1381 aaatatataa aagtgaatta aataagcaca tagaggatgg tatgggaaga aatttggctg 1441 atcgatgcac cgatgaagta aacgccttag tgcttcagac ccagcaagaa attattgaaa 1501 atttgaagcc attacttcca gctggtatac aggataaact acatacactg atcccttgca 1561 agaaatttga tctcagttat aatctaaatt accacaagtt atgttcagat tttcaagagg 1621 atattgtatt tcgtttttcc ctgggctggt cttcccttgt acatcgattt ttgggcccta 1681 gaaatgctca aagggtgctc ctaggattat cagagcctat ctttcagctc cctagatctt 1741 tagcttctac tcccactgct cctaccactc cagcaacgcc agataatgca tcacaggaag 1801 aactcatgat tacattagta acaggattgg cgtccgttac atctagaact tctatgggca 1861 tcattattgt tggaggagtg atttggaaaa ctataggctg gaaactccta tctgtttcat 1921 taactatgta tggagctttg tatctttatg aaagactgag ctggaccacc catgccaagg 1981 agcgagCCTT TAAACAGCAG TTTGTAAACT ATGCAACTGA AAAACTGAGG ATGATTGTTA 2041 GCTCCACGAG TGCAAACTGC AGTCACCAAG TAAAACAACA AATAGCTACC ACTTTTGCTC 2101 GCCTGTGCCA ACAAGTTGAT ATTACTCAAA AACAGCTGGA AGAAGAAATT GCTAGATTAC 2161 CCAAAGAAAT AGATCAGTTG GAGAAAATAC AAAACAATTC AAAGCTCTTA AGAAATAAAG 2221 CTGTTCAACT TGAAAATGAG CTGGAGAATT TTACTAAGCA GTTTCTACCT TCAAGCAATG 2281 AAGAATCCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2341 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2401 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTTCTA CTTTGAACGT AGCCTTAAAC 2461 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt