Construct: ORF TRCN0000478634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015468.2_s317c1
- Derived from:
- ccsbBroadEn_07720
- DNA Barcode:
- ATATACCACATAAATGTAGTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ILVBL (10994)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10994 | ILVBL | ilvB acetolactate synthase ... | NM_006844.5 | 99.9% | 100% | 1035T>C |
| 2 | human | 10994 | ILVBL | ilvB acetolactate synthase ... | XM_005259717.4 | 99.9% | 100% | 1035T>C |
| 3 | human | 10994 | ILVBL | ilvB acetolactate synthase ... | XM_024451330.1 | 95.5% | 95.6% | 602_688del;1122T>C |
| 4 | human | 10994 | ILVBL | ilvB acetolactate synthase ... | XM_011527651.2 | 83% | 83% | 0_1ins321;714T>C |
| 5 | mouse | 216136 | Ilvbl | ilvB (bacterial acetolactat... | NM_173751.4 | 84.4% | 86.3% | (many diffs) |
| 6 | mouse | 216136 | Ilvbl | ilvB (bacterial acetolactat... | XM_011243400.2 | 84.4% | 86.3% | (many diffs) |
| 7 | mouse | 216136 | Ilvbl | ilvB (bacterial acetolactat... | XM_011243399.2 | 83.1% | 85% | (many diffs) |
| 8 | mouse | 216136 | Ilvbl | ilvB (bacterial acetolactat... | XM_011243401.1 | 53.8% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1962
- ORF length:
- 1896
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacccccgcg gccgccgccc ccgctgggag cttattcccc tccttcctgc 121 tcctggcctg cgggacgctg gtggccgcct tgctgggcgc cgctcaccgc ctggggctct 181 tctatcagct gctgcacaag gtggacaagg caagcgtccg gcatggcgga gagaacgtgg 241 ccgctgtgct gagggcccat ggtgtgcggt tcatcttcac gctggtcggt gggcacattt 301 ccccgctgct ggtggcctgt gagaaactgg gcatccgtgt ggtggacaca cgccatgagg 361 tcacggccgt ctttgctgct gatgctatgg cccgcctgtc cgggacggtg ggcgtggcgg 421 cagtgacagc aggccctggc ctcaccaaca cggtgactgc ggtgaagaat gctcagatgg 481 ctcagtcccc aatcctgctt ctgggtgggg ctgccagcac tctgctgcag aaccggggtg 541 cgctccaggc tgttgatcag ctgtcccttt tccggccact ctgtaagttt tgtgtgtctg 601 tgcggagggt gcgggacatt gtgcccaccc tgagggccgc gatggctgcc gcccagtcgg 661 gcaccccagg tccggtgttt gtggagctgc ccgttgacgt gctgtacccc tacttcatgg 721 tccagaagga gatggtgcca gccaagccac ccaagggcct cgtgggccga gtggtctcct 781 ggtatttaga gaattacctg gccaacctct ttgcaggagc ctgggagcct cagcccgagg 841 gaccgctgcc cctggacatc ccccaggctt ccccgcagca ggttcagcgc tgtgtggaga 901 tcctgagccg ggccaagagg cctctgatgg tgctggggag tcaggccctg ctcaccccaa 961 cgtctgccga caagcttcgg gctgccgtgg agaccttggg tgttccctgc ttccttggag 1021 ggatggcacg ggggctgtta ggccgcaacc accccctcca catccgggag aaccgcagtg 1081 cggccctgaa gaaggcggac gtcattgtcc tagcaggaac tgtgtgtgac ttccgcctat 1141 cctatggccg tgtcctcagc cacagcagca agatcatcat cgtcaatcgt aatcgggaag 1201 agatgttgct caactcagac atcttctgga agccccagga ggctgtgcag ggagatgtgg 1261 gttccttcgt gctgaagtta gtggagggcc ttcagggcca gacctgggcc ccagactggg 1321 tggaggagct gcgggaagcc gaccggcaga aggagcagac ctttcgggag aaggcagcga 1381 tgcctgtggc ccagcacctg aacccagtgc aggtgctgca gctggtggag gaaacgctac 1441 ctgacaactc aattctggtg gtggatggcg gggacttcgt gggcactgct gcccatctgg 1501 tacagccccg cggccccctg cgctggctcg atcctggggc ctttgggact ctgggagttg 1561 gtgcaggatt tgcacttggg gccaagctgt gccggccaga tgctgaggtc tggtgcctgt 1621 ttggggacgg agcttttggc tacagccTCA TCGAATTTGA TACATTCGTC AGACACAAGA 1681 TCCCAGTGAT GGCCTTGGTA GGGAATGATG CTGGCTGGAC ACAGATTTCT CGGGAGCAGG 1741 TGCCCTCTCT GGGCAGCAAC GTGGCCTGTG GCCTGGCCTA CACTGATTAT CACAAGGCAG 1801 CCATGGGTCT GGGGGCCCGG GGCTTGCTGC TCTCACGGGA GAACGAGGAT CAGGTGGTCA 1861 AGGTGCTGCA CGATGCCCAG CAGCAGTGCC GAGACGGCCA CCCGGTTGTG GTCAACATCC 1921 TCATTGGGAG GACGGACTTC CGCGATGGCT CCATTGCTGT ATACCCAACT TTCTTGTACA 1981 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2041 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2101 AGGACGAATA TACCACATAA ATGTAGTGGC GACGCGTTAA GTCgacaatc aacctctgga 2161 ttacaaaatt tgtgaaagat t